36,707 research outputs found
A Cross-Sectional Study of People with Epilepsy and Neurocysticercosis in Tanzania: Clinical Characteristics and Diagnostic Approaches.
No full textNeurocysticercosis (NCC) is a major cause of epilepsy in regions where pigs are free-ranging and hygiene is poor. Pork production is expected to increase in the next decade in sub-Saharan Africa, hence NCC will likely become more prevalent. In this study, people with epilepsy (PWE, n=212) were followed up 28.6 months after diagnosis of epilepsy. CT scans were performed, and serum and cerebrospinal fluid (CSF) of selected PWE were analysed. We compared the demographic data, clinical characteristics, and associated risk factors of PWE with and without NCC. PWE with NCC (n=35) were more likely to be older at first seizure (24.3 vs. 16.3 years, p=0.097), consumed more pork (97.1% vs. 73.6%, p=0.001), and were more often a member of the Iraqw tribe (94.3% vs. 67.8%, p=0.005) than PWE without NCC (n=177). PWE and NCC who were compliant with anti-epileptic medications had a significantly higher reduction of seizures (98.6% vs. 89.2%, p=0.046). Other characteristics such as gender, seizure frequency, compliance, past medical history, close contact with pigs, use of latrines and family history of seizures did not differ significantly between the two groups. The number of NCC lesions and active NCC lesions were significantly associated with a positive antibody result. The electroimmunotransfer blot, developed by the Centers for Disease Control and Prevention, was more sensitive than a commercial western blot, especially in PWE and cerebral calcifications. This is the first study to systematically compare the clinical characteristics of PWE due to NCC or other causes and to explore the utility of two different antibody tests for diagnosis of NCC in sub-Saharan Africa
Myopites Blot 1827
No full textGenus MYOPITES Blot Myopites Blot, 1827: 102. Type species: Myopites inulaedyssentericae Blot, 1827, monotypic. Rhyncheterus Rondani, 1863: 37. Type species: Rhyncheterus damascenus Rondani, 1863 (= Myopites inulaedyssentericae Blot, 1827), monotypy. Myiopites Bezzi, 1908: 140. Misspelling of Myopites. Rhynchetenus Foote, 1984: 99. Misspelling of Rhyncheterus. Myipites Foote, 1984: 99. Misspelling of Myopites Blot, attributed to “authors”.Published as part of El-Hawagry, Magdi S., 2017, Catalogue of Egyptian Tephritoidea (Diptera: Schizophora: Acalyptratae), pp. 151-190 in Zootaxa 4299 (2) on page 164, DOI: 10.11646/zootaxa.4299.2.1, http://zenodo.org/record/84323
A well-conserved Plasmodium falciparum var gene shows an unusual stage-specific transcript pattern
No full textThe var multicopy gene family encodes Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) variant antigens, which, through their ability to adhere to a variety of host receptors, are thought to be important virulence factors. The predominant expression of a single cytoadherent PfEMP1 type on an infected red blood cell, and the switching between different PfEMP1 types to evade host protective antibody responses, are processes thought to be controlled at the transcriptional level. Contradictory data have been published on the timing of var gene transcription. Reverse transcription-polymerase chain reaction (RT-PCR) data suggested that transcription of the predominant var gene occurs in the later (pigmented trophozoite) stages, whereas Northern blot data indicated such transcripts only in early (ring) stages. We investigated this discrepancy by Northern blot, with probes covering a diverse var gene repertoire. We confirm that almost all var transcript types were detected only in ring stages. However, one type, the well-conserved varCSA transcript, was present constitutively in different laboratory parasites and does not appear to undergo antigenic variation. Although varCSA has been shown to encode a chondroitin sulphate A (CSA)-binding PfEMP1, we find that the presence of full-length varCSA transcripts does not correlate with the CSA-binding phenotype
The Blot
No full textTheblotisasigninPeirce’sdiagrammaticsyntaxofexistentialgraphs that has hitherto been neglected in the literature on logical graphs. It is needed in order to trigger the cut-as-negation to come out from the scroll, namely from the implicational sign of a positive implicational (paradisiacal) logic. Since the cut-as-negation presupposes the blot and the scroll, what does the blot represent? On the one hand, it stands for constant absurdity, but on the other hand, Peirce takes it to be an affirmative sign. This paper explores the blot and its logical and conceptual properties from the multiple perspectives of notation, rules of transformation, icons, and scriptibility of graphs. It explains the apparent conflict in the blot’s meaning in its capacity of giving rise to the pseudo-graph that exploits positive character of absurdity. In effect, the blot is the mirror image of the sheet of assertion, not its complementation. On the sheet, it acts as a non-juxtaposable singularity
Evaluation of immuno-dot-blot assay for detection of cholera-related enterotoxin antigen in Salmonella typhimurium
No full textTwenty-five strains of Salmonella typhimurium isolated in India were examined for the presence of cholera/coli-related enterotoxin antigen by a previously described latex particle agglutination test and by a newly developed immuno-dot-blot test using immunopurified goat antibody against the cholera-related enterotoxin isolated from an Escherichia coli strain of human origin. The immuno-dot-blot assay could detect 0.02 ng of purified enterotoxin. The amount of toxin antigen detected varied widely from strain to strain. Fourteen of the 25 polymyxin B-treated extracts of bacteria harvested from 6-h Casamino Acids-yeast extract broth cultures gave positive results in both serologic assays as well as in rabbit skin tests for delayed permeability factor. An additional strain was positive only in the immuno-dot-blot. Five of six stool isolates and six of seven blood isolates tested gave positive reactions. Two isolates of Salmonella enteritidis tested were also positive. The immuno-dot-blot test appears to be a simple, rapid, and reliable method for detection of cholera-related enterotoxin antigen in S. typhimurium. The demonstration of a cholera-related enterotoxin, even in small amounts, in a facultative intracellular pathogen raises interesting questions regarding its potential role in pathogenesis both of diarrheal disease and systemic infections caused by salmonellae.LR: 20061115; PUBM: Print; JID: 7505564; 0 (Antigens, Bacterial); 0 (Bacterial Toxins); 0 (Endotoxins); 0 (Enterotoxins); 0 (Escherichia coli Proteins); 0 (enterotoxin LT); 0 (salmonella toxin); 0 (stN protein, Vibrio cholerae); ppublishSource type: Electronic(1
Stable tree expressions with Omega-classes and double ramification cycles
No full textWe propose a new system of conjectural relations in the tautological ring of the moduli space of curves involving stable rooted trees with level structure decorated by Hodge and Ω-classes and prove these conjectures in different cases
Detection of bovine herpesvirus 1 in the semen of experimentally infected bulls by dot-blot hybridisation, polymerase chain reaction and virus isolation
No full textTwo 18-month-old bovine herpesvirus 1 (BHV1)-seronegative bulls were inoculated experimentally with BHV1 via their prepuces. Semen collected at intervals was examined by optimised virus isolation, dot-blot hybridisation and the polymerase chain reaction (PCR) for detection of BHV1, and the infection was monitored serologically by using a virus neutralisation test. Antibodies were first detected 10 days after inoculation and were still present 40 days after inoculation. Semen collected from four to 40 days after inoculation was positive by PCR with Southern blot hybridisation whereas only the semen collected on day 4 was positive by dot-blot hybridisation, virus isolation and PCR with ethidium bromide staining. These results indicate that the bulls started to shed the virus in semen before they developed any detectable antibody. PCR with Southern blot hybridisation was the most sensitive of the three methods and detected virus for the longest period.LR: 20061115; PUBM: Print; JID: 0401300; 0 (Antibodies, Viral); ppublishSource type: Electronic(1
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