172 research outputs found
Concorso di progettazione per la riqualificazione di Piazza Garibaldi a Velletri
No full textVELLETRI (Roma), Concorso nazionale di progettazione: Piazza Cairoli, Piazza Garibaldi “Velletri 2001”, indetto dal Comune di Velletri per la progettazione di riqualificazione delle due piazze. Progetto per Piazza Garibaldi.
Gruppo di progettazione: G. Palmerio (capogruppo), S. Belelli, A. Di Muzio, R. Mancini
Towards a dialectology of Southern Kurdish: Where to begin?
No full textThis contribution provides an overview of the current state of knowledge on the dialectology of Southern Kurdish (hereafter SK). The introductory paragraphs discuss the concept of SK, survey existing sources and briefly address core issues of terminology. The bulk of the study reviews Fattah’s (2000: 9) proposed dialect classification and complements it with the evaluation of language data from older sources, the author’s own research in Kermānshāh Province and other documentation activities recently carried out in the SK-speaking area, sketching possible directions for future research
The general anaesthetic etomidate inhibits the excitability of mouse thalamocortical relay neurons by modulating multiple modes of GABA<sub>A</sub> receptor-mediated inhibition
No full textModulation of thalamocortical (TC) relay neuron function has been implicated in the sedative and hypnotic effects of general anaesthetics. Inhibition of TC neurons is mediated predominantly by a combination of phasic and tonic inhibition, together with a recently described ‘spillover’ mode of inhibition, generated by the dynamic recruitment of extrasynaptic ?-aminobutyric acid (GABA)A receptors (GABAARs). Previous studies demonstrated that the intravenous anaesthetic etomidate enhances tonic and phasic inhibition in TC relay neurons, but it is not known how etomidate may influence spillover inhibition. Moreover, it is unclear how etomidate influences the excitability of TC neurons. Thus, to investigate the relative contribution of synaptic (a1ß2?2) and ex-trasynaptic (a4ß2d) GABAARs to the thalamic effects of etomidate, we performed whole-cell recordings from mouse TC neurons lacking synaptic (a10/0) or extrasynaptic (d0/0) GABAARs. Etomidate (3µM) significantly inhibited action-potential discharge in a manner that was dependent on facilitation of both synaptic and extrasynaptic GABAARs, although enhanced tonic inhibition was dominant in this respect. Additionally, phasic inhibition evoked by stimulation of the nucleus reticularis exhibited a spillover component mediated by d-GABAARs, which was significantly prolonged in the presence of etomidate. Thus, etomidate greatly enhanced the transient suppression of TC spike trains by evoked inhibitory postsynaptic potentials. Collectively, these results suggest that the deactivation of thalamus observed during etomidate-induced anaesthesia involves potentiation of tonic and phasic inhibition, and implicate amplification of spillover inhibition as a novel mechanism to regulate the gating of sensory information through the thalamus during anaesthetic states.</p
Civitavecchia (Roma), loc. Scarti di S. Antonio. Esplorazione preliminare di un nuovo contesto sacro
No full textIn 1999 some fragments of architectural decorations, found by a private citizen in the locality of Scarti di Sant’Antonio (Civitavecchia, Rome), were presented to the Soprintendenza per i Beni Archeologici dell’Etruria Meridionale by the Guardia di Finanza.
In 2003 an investigation of the area in which the fragments were discovered was possible thanks to the project “Grandi Santuari”.
This paper is the report of this field campaign: both the excavation and the findings are discussed. The chronological sequence clearly speaks of a continuous use of the S. Antonio area from at least the Archaic period onward. Particular attention is paid in the paper to the architectural fragments, whose discovery revealed the presence in the Civitavecchia area of an up-to-now unknown sacred building that can be dated to the Hellenistic period and was decorated with terracottas possibly representing the episode of the hunt of the Calydonian boar.
The area was then occupied by a Roman villa, whose installation is connected to the reorganization of the whole hill with a complex arrangement of terraces and that must be read in connection with the complex process of “Romanization” of the southern Etruscan coastal area
Tra Caere e Pyrgi. Nuove ricerche in località Montetosto
No full texthe site of Montetosto is located 4 km to the west of Caere, along the monumental road that connected the
Etruscan city to its main port at Pyrgi. Until 1962 the site was known only for the visible tumulus – presently
the largest known in Etruria – excavated by Raniero Mengarelli between 1924 and 1929.
A short season of geophysical survey was conducted in March 2018 in close collaboration with the Soprintendenza
Archeologia, Belle Arti e Paesaggio per l’area metropolitana di Roma, la provincia di Viterbo
e l’Etruria Meridionale. An area of 1.07 ha was surveyed between the Montetosto tumulus and the eastern
edge of the previously excavated building.
The magnetometry survey highlighted the presence of a substantial series of additional walls and features in
the eastern sector of the complex in an area not fully explored in the excavations of the 1960s. In the northern
part of the survey area the magnetometry precisely mapped the perimeter of the west side of the tumulus
which is now buried, confirming a diameter of 65 m. On the exposed eastern side of the monument several
red tuff slabs which formed part of the base of the monument were visible.
The route of the Caere–Pyrgi road between the tumulus and the sacred building was suggested by the topographical
surveys carried out in the 1960s. The fieldwork indicated the general trajectory of the road, but it
was clear that in the vicinity of Montetosto it had already undergone substantial destruction. The magnetometry
has highlighted the presence of two parallel anomalies to the north-east of the sanctuary which may
be associated with the Caere-Pyrgi, finding also an interesting parallelism in the situation highlighted thanks
to the new topographical research carried out by C.B. De Vita on the Montetosto area
Toward a Unified TreeTalker Data Curation Process
No full textThe Internet of Things (IoT) development is revolutionizing environmental monitoring and research in macroecology. This technology allows for the deployment of sizeable diffuse sensing networks capable of continuous monitoring. Because of this property, the data collected from IoT networks can provide a testbed for scientific hypotheses across large spatial and temporal scales. Nevertheless, data curation is a necessary step to make large and heterogeneous datasets exploitable for synthesis analyses. This process includes data retrieval, quality assurance, standardized formatting, storage, and documentation. TreeTalkers are an excellent example of IoT applied to ecology. These are smart devices for synchronously measuring trees’ physiological and environmental parameters. A set of devices can be organized in a mesh and permit data collection from a single tree to plot or transect scale. The deployment of such devices over large-scale networks needs a standardized approach for data curation. For this reason, we developed a unified processing workflow according to the user manual. In this paper, we first introduce the concept of a unified TreeTalker data curation process. The idea was formalized into an R-package, and it is freely available as open software. Secondly, we present the different functions available in “ttalkR”, and, lastly, we illustrate the application with a demonstration dataset. With such a unified processing approach, we propose a necessary data curation step to establish a new environmental cyberinfrastructure and allow for synthesis activities across environmental monitoring networks. Our data curation concept is the first step for supporting the TreeTalker data life cycle by improving accessibility and thus creating unprecedented opportunities for TreeTalker-based macroecological analyses
Carico pratico ed emotivo delle famiglie in relazione alla psicopatologia e all’adattamento sociale di psicotici cronici lungoassistiti
No full text
The effect of a transmembrane amino acid on etomidate sensitivity of an invertebrate GABA receptor
No full text1. The gamma-aminobutyric acid (GABA)-modulatory and GABA-mimetic actions of etomidate at mammalian GABA(A) receptors are favoured by beta2- or beta3- versus beta1-subunit containing receptors, a selectivity which resides with a single transmembrane amino acid (beta2 N290, beta3 N289, beta1 S290). Here, we have utilized the Xenopus laevis oocyte expression system in conjunction with the two-point voltage clamp technique to determine the influence of the equivalent amino acid (M314) on the actions of this anaesthetic at an etomidate-insensitive invertebrate GABA receptor (Rdl) of Drosophila melanogaster. 2. Complementary RNA-injected oocytes expressing the wild type Rdl GABA receptor and voltage-clamped at -60 mV responded to bath applied GABA with a concentration-dependent inward current response and a calculated EC50 for GABA of 20+/-0.4 microM. Receptors in which the transmembrane methionine residue (M314) had been exchanged for an asparagine (RdlM314N) or a serine (RdlM314S) also exhibited a concentration-dependent inward current response to GABA, but in both cases with a reduced EC50 of 4.8+/-0.2 microM. 3. Utilizing the appropriate GABA EC10, etomidate (300 microM) had little effect on the agonist-evoked current of the wild type Rdl receptor. By contrast, at RdlM314N receptors, etomidate produced a clear concentration-dependent enhancement of GABA-evoked currents with a calculated EC50 of 64+/-3 microM and an Emax of 68+/-2% (of the maximum response to GABA). 4. The actions of etomidate at RdlM314N receptors exhibited an enantioselectivity common to that found for mammalian receptors, with 100 microM R-(+)-etomidate and S-(-)-etomidate enhancing the current induced by GABA (EC10) to 52+/-6% and 12+/-1% of the GABA maximum respectively. 5. The effects of this mutation were selective for etomidate as the GABA-modulatory actions of 1 mM pentobarbitone at wild type Rdl (49+/-4% of the GABA maximum) and RdlM314N receptors (53+/-2% of the GABA maximum) were similar. Additionally, the modest potentiation of GABA produced by the anaesthetic neurosteroid 5alpha-pregnan-3alpha-ol-20-one (Rdl = 25+/-4% of the GABA maximum) was not altered by this mutation (RdlM314N = 18+/-3% of the GABA maximum). 6. Etomidate acting at beta1 (S290)-containing mammalian GABA(A) receptors is known to produce only a modest GABA-modulatory effect. Similarly, etomidate acting at RdlM314S receptors produced an enhancement of GABA but the magnitude of the effect was reduced compared to RdlM314N receptors. 7. Etomidate acting at human alpha6beta3gamma2L receptors is known to produce a large enhancement of GABA-evoked currents and at higher concentrations this anaesthetic directly activates the GABA(A) receptor complex. Mutation of the human beta3 subunit asparagine to methionine (beta3 N289M found in the equivalent position in Rdl completely inhibited both the GABA-modulatory and GABA-mimetic action of etomidate (10-300 microM) acting at alpha6beta3 N289Mgamma2L receptors. 8. It was concluded that, although invertebrate and mammalian proteins exhibit limited sequence homology, allosteric modification of their function by etomidate can be influenced in a complementary manner by a single amino acid substitution. The results are discussed in relation to whether this amino acid contributes to the anaesthetic binding site, or is essential for transduction. Furthermore, this study provides a clear example of the specificity of anaesthetic action
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