650 research outputs found
Detection of Five Common CFTR Mutations by Rapid-Cycle Real-Time Amplification Refractory Mutation System PCR
No full textCystic fibrosis is the most common autosomal recessive disease in Caucasian populations and has a carrier frequency of 1 in 25 (1 ). The gene involved codes for the cystic fibrosis transmembrane conductance regulator (CFTR), a membrane-associated protein involved in ion transport across the plasma membrane of epithelial cells. To date more than 1000 mutations have been described in this gene, and most are rare (2 ). By focusing on five common mutations it is possible to detect the disease causing mutation in 90% of patients (3
Climate change and equity
No full textThe Gavin Mooney Memorial Essay Competition honours the work and memory of the late Professor Gavin Mooney, a health economist who was a tireless advocate for social justice in local, national and international arenas.
Launched in 2013, the competition seeks to draw public attention to social justice and health equity concerns, and to recognise the public-interest value of writing and writers.
The inaugural competition called for essays on the theme of climate change and equity, in recognition of the work of Professor Mooney’s late partner Dr Delys Weston. Each year the competition will call for entries related to a theme around equity and social justice.
Sydney GP Dr Tim Senior took out the inaugural 2013 prize with his essay “Climate Change and Equity: Whose Language Is It Anyway?”. The judges said the winning entry challenges the language of climate change activism, and also incorporates the voices of those who are most likely to be affected by climate change.
The four runner-up entries are by Steve Campbell and Lucie Rychetnik, Oscar McLaren, Peter Boyer and Dora Marinova, and Fergus Green
Development and Validation of a Novel Reporter Assay for Human Papillomavirus Type 16 Late Gene Expression
No full textTo facilitate the investigations of HPV-16 late gene expression HPV-16 reporter plasmids were generated using previously described sub-genomic HPV-16 plasmids, named pBEL and pBELM, that, similar to the full viral genome, produce primarily HPV-16 early mRNAs and very little, if any, late mRNAs in cervical cancer cells. The HPV-16 late L1 gene was replaced by the chloramphenicol acetyltransferase (CAT) reporter gene, or green fluorescent protein (GFP), preceded by the poliovirus internal ribosome entry site (IRES). Results show that the reporter genes mimic the expression of L1 from these plasmids. For example, overexpression of adenovirus E4orf4 protein (E4orf4), polypyrimidine tract binding protein (PTB), arginine/serinerich SRp30c protein (SRp30c) or alternative splicing factor/splicing factor 2 (ASF/SF2) induced an increased expression of CAT or GFP. Stable cell lines with reporter plasmids pBELCAT and pBELMCAT were also generated. An induction of CAT was observed in HPV-16 reporter cell lines in the presence of the small molecule phorbol 12-myristate 13-acetate (TPA). Further experiments identified the TPA-inducible, hnRNP A2/B1 protein as a regulator of HPV-16 late gene expression. In conclusion, the HPV-16 reporter plasmids and reporter cell lines described herein can be used to identify small molecules and cellular factors that regulate HPV-16 gene expression
Retirement of a Coal-Generated Electric Power Plant in Fergus Falls
No full textExtension's research is informed by the programs and partnerships we have with communities across the state. Learn more at extension.umn.edu. This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu.Tuck, Brigid; Chitrakar, Situ; Pesch, Ryan. (2018). Retirement of a Coal-Generated Electric Power Plant in Fergus Falls. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/202020
An Evaluation of MIRU-VNTR Analysis and Spoligotyping for Genotyping of Mycobacterium Bovis Isolates and a Comparison with RFLP Typing.
No full textStrain typing of Mycobacterium bovis isolates based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) and on spoligotyping was evaluated in this study and these typing methods were compared with restriction fragment length polymorphism (RFLP) typing. A total of 386 M. bovis isolates from cattle, badgers and deer in the Republic of Ireland that had previously been typed by IS6110, polymorphic GC-rich sequence (PGRS) and direct repeat (DR) RFLP were included in the study. Spoligotyping and analysis of six VNTR loci (2163a, 2163b, 2165, 4052, 2996 and 1895) was performed on the samples. RFLP was the method that gave the greatest differentiation of strains with a Hunter Gaston discriminatory index (HGDI) of 0.927, the HGDI recorded for MIRU-VNTR was marginally lower at 0.918 and spoligotyping was the least discriminatory method with a HGDI of 0.7. Spoligotype SB0140 represented approximately 50% of the isolates. Within the group of isolates represented by SB0140 there was a much lower level of concordance between RFLP and MIRU-VNTR typing compared to groups represented by other spoligotypes. A combination of spoligotyping and MIRU-VNTR typing offered advantages over MIRU-VNTR typing alone. In a combined spoligotyping and MIRU-VNTR typing protocol the number of VNTR loci could be reduced to four (2163a, 2163b 2165 and 4052) while maintaining a high level of strain differentiation
An overlapping spheres model of cell-cell interactions
No full textCode for a Part B BSP student project in the Mathematical Institute, University of Oxford supervised by Fergus Cooper.
The main author is not yet attributed to maintain anonymity until after the project has been graded
A Fergus Hume Novel's Occult Folklore and the Ancient Continent of Lemuria
No full text"[He was] wondering what thought hovered at the hack of his brain about Easter Island. He had visited that lonely and little known spot during his travels in the company of a friend given to occult studies, who insisted that the dismal spot of land was one of the remaining portions of the great Continent of Lemuria, which was said to have stretched from New Zealand to Africa." --Fergus Hume, 'The Sacred Herb' (1908), p. 41. "'This cup', said Horace, raising it aloft, 'is thousands and thousands of years old. It is a remnant of Lemurian civilization.'" --op. cit., p. 319. These quotations come from near the beginning and end, respectively, of one of the more surprising middle-period novels from the prolific Fergus Hume (1859-1932), author of 'The Mystery of a Hansom Cab' (1886), and sometime resident of Melbourne, where his remarkable literary career began. Appropriately both the Victorian and New South Wales State Libraries in Australia hold copies of this rare text, 'The Sacred Herb' which is filled with passing references to: Australia (p. 274); New Zealand (p. 41), and Hokitika (p. 18) in particular; Polynesia (passim and especially p. 294); the South Seas, Samoa, Tahiti and the British Empire and its colonies
Melbourne's Century Old Mystery - Who was Fergus Hume?
No full textOne hundred years ago this year, there arrived on the Melbourne scene from overseas a young man who was to use his new, if temporary, home city and his own remarkable cluster of talents to produce one of the best-selling popular novels of all time, - 'The Mystery of a Hansom Cab'. It's publication has been estimated to exceed two thirds of a million copies. Quite certainly he put a mark on the then colonial city which gave it, at its age of fifty a distinctive if brash, Victorian image, which has gone around the world since that time. The writer was, of course. 'Fergus Hume' (1859-1932), of Scottish stock, born Fergusson Wright Hume on 8 July 1859 at the Royal Malvern Hospital, Worcester where his father was the steward to the mentally disturbed. With his parents, brother and sisters, 'Fergus' as he was always known, landed in January 1863, at Port Chalmers, New Zealand, the family drawn thither not by the magnet of the recent gold strikes in the interior so much as the fact that Otago was, still, in foundation and temper, a Presbyterian colony - to which faith his father James adhered till his dying day. In Dunedin James soon became the manager of the city's lunatic asylum, a figure of substance, who was concerned to give his younger son, Fergus, an excellent traditional education, first at the High School, a Scottish foundation, and then at the University of Otago, where he fell under the dynamic influence of Professor George Sale, a former classics tutor of Trinity College, Cambridge. Actually, however, Fergus's formal studies were 1n law, and he was articled to Robert Stout, then a famed criminal lawyer and attorney-general, but soon to become premier of New Zealand
As I rode out one evening, down by a shady side
No full textAs a young man rides out he meets a lovely young woman. He offers to marry her, but she says she is already engaged. Jenny's lvoer is overseas at war. The young man says that if Jenny's lover is Dinny Ryan from New York, then he is dead. Jenny faints - and then the young man reveals himself as Dinny Ryan and swears to marry Jenny.See Greenleaf & Mansfield, p. 180
Generative justice: Beyond crime and punishment.
No full textThis is a post-peer-review, pre-copy edited version of a chapter published in Generative justice: Beyond crime and punishment. The definitive publisher-authenticated version [Murray, E., Arias, L., Buck, G., Ryan, K., & Ryan, N. (2026). Generative justice in hindsight: On knowing, doing, and sharing participatory arts-based research with criminal justice-impacted communities. In F. McNeill, M. Corcoran, & B. Weaver (Eds.), Generative justice: Beyond crime and punishment. BUP.] is available online at: [DOI available on publication].This chapter re-examines two very different participatory arts-based projects within the justice sector through the lens of Generative Justice (hereafter GJ), an emerging concept-praxis that integrates prefigurative (seed-planting) community practices with criminal justice-impacted individuals to reimagine relationships based on solidarity rather than exclusion (McNeill, 2022; McNeill, Corcoran and Weaver, 2023). GJ constitutes a disruptive social change effort, which, like participatory arts-based projects, involves ‘shifting communities and networks’ working toward localised social transformation (Frödén, 2023: 104). In fact, ‘arts-based interventions have played a key role in enriching and mobilising prefigurative movements all over the world’ (Frödén, 2023: 105). As authors, we have been involved in participatory arts-based research and praxis that aims to challenge and transform power structures by leveraging community-driven creativity. Each of our works were designed to place lived experience of criminal justice systems at the centre of knowledge production, working with imagination to create diverse research communities based on solidarity, hope and systemic change (McNeill, 2022). Despite differences between our two projects, both align with GJ, sharing interests in reciprocity, generativity, active participation, and collective action.UKRI FLF MR/Y017110/
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