86,595 research outputs found
Induction of Apoptosis by Chlamydia psittaci and Chlamydia trachomatis Infection in Tissue Culture Cells
No full textThe role of programmed cell death (apoptosis) in LLC-MK2 cells infected with Chlamydia trachomatis LGV2 serotype and Chlamydia psittaci 6BC strain was investigated using flow cytometry and TUNEL procedures. The number of apoptotic cells was significantly higher at 72 and 96 hours post infection in the Chlamydia infected cell cultures in comparison with mock-infected cells. We postulate the apoptotic process to be a mechanism induced by C. trachomatis and C. psittaci infection in LLC-MK2 cells
Prevalence of human papillomavirus (HPV) types 6b-11 and 16-18 in genital condylomas
No full textAbstract in Englis
An immunoperoxidase assay for the detection of specific IgA antibody in Epstein-Barr virus infections.
No full textA technique using indirect immunoperoxidase antibody was developed for the detection of specific serum IgA antibody to Epstein-Barr virus capsid antigen and early antigen. The IgA technique was compared with an immunofluorescence antibody method. Epstein-Barr virus IgA antibody against viral capsid antigen was detected in all nine patients with Epstein-Barr virus associated undifferentiated nasopharyngeal carcinoma, in 13 (72.2%) of 18 patients with infectious mononucleosis, in 21 (28.3%) of 74 patients with acute lymphoblastic leukaemia, and in six (20%) of 30 patients who had recently had kidney transplants. Epstein-Barr virus IgA antibody against viral capsid antigen was also detected in four (10%) of 40 healthy subjects, but it was not found in any of 20 cord blood samples. Epstein-Barr virus IgA antibody to early antigen was detected in six (66.6%) patients with nasopharyngeal carcinoma and in two (2.7%) patients with acute lymphoblastic leukaemia. The immunoperoxidase assay for Epstein-Barr virus specific IgA was simple, reliable, and rapid and correlated well (r = 0.94) with the immunofluorescence antibody technique
Screening dell’infezione tubercolare latente in operatori sanitari con QuantiFERON-TB e test cutaneo tubercolinico [Screening of latent tuberculosis infection in health care workers by QuantiFERON-TB and tuberculin skin test]
No full textBackground. Recent guidelines (MMWR 2005) recommend the use of QuantiFERON-TB (QFT-TB) as an alternative to the tuberculin skin test (TST) for the screening of latent tuberculosis infection (LTBI) in health care workers (HCWs). Materials and methods. 590 HCWs were screened for LTBI by TST and QFT-TB. Results were compared with risk factors for LTBI, determined by questionnaires. Results. Both tests were significantly associated with non-Italian nationality [TCT (OR=9.17), QFT-TB (OR=3.65)], age ≥ 45 years old [TCT (OR=1.81), QFT-TB (OR=2.36)], history of household contacts [TCT (OR=2.65), QFT-TB (OR=3.37], occupational exposure to tuberculosis (TB) patients [TCT (OR=2.14), QFT-TB (OR=1.93)]. 55 cases were discordant (28 QFT-TB-negatives/TCT-positives; 27 QFT-TB-positives/TCT-negatives). Both tests were not associated with workplace risk factors or TB risk level assessed in different hospital units. Conclusions. In HCWs employed in a low TB incidence area both QFT-TB and TCT were more associated with non occupational risk factors (nationality, age, household contacts) than with main determinants of workplace risk for LTBI
Valutazione di un nuovo test di agglutinazione al lattice per la diagnosi delle gastroenteriti da rotavirus
No full text
A simple immunoperoxidase method for detecting enteric adenovirus and rotavirus in cell culture.
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