3 research outputs found
Quaternary Segmentation Characteristics of the Hunhe Fault, Northeast China
No full textThe northern segment of the Tanlu fault zone, which encompasses the Dunhua–Mishan and Yilan–Yitong fault zones, plays a critical role in the tectonic framework of Northeast China. This study focuses on the Hunhe fault, part of the Liaoning segment of the Dunhua–Mishan fault zone, which exhibits concealed characteristics and an NE–NEE orientation. We employ remote sensing and field investigations to accurately delineate the Hunhe fault’s location, scale, and tectonic activity. The findings indicate that the Hunhe fault displays significant spatial variability in tectonic activity. Some segments show evidence of late Quaternary activity, contradicting prior research that classified the Hunhe fault as an active fault during the MIS (Marine Isotope Stages) 20-103MIS 20-103- MIS6-19MIS6-19 period and assessed its seismic potential differently. Recent field investigations suggest considerable spatial variability in tectonic activity, indicating segmental characteristics. In this study, the Hunhe fault is divided into segments based on five aspects: the fault structure and movement characteristics of the fault; transverse faults and obstruction structures; geological and geomorphological characteristics; seismic features; and fault activity. The detailed segments are as follows: the Shenyang segment, the Fushun segment, the Zhangdang-Nan Zamu segment, and the Nan Zamu to Ying Emeng East section. These findings aim to enhance the understanding of the seismic hazard potential associated with the Hunhe fault, highlighting the need for ongoing research to address its complexities and implications for regional seismic risk assessment
Identification of microRNAs and their targets associated with fruit-bagging and subsequent sunlight re-exposure in the ‘Granny Smith’ apple exocarp using high-throughput sequencing
No full textBagged fruits of green apple cultivar ‘Granny Smith’ have been found to turn cardinal red after debagging during fruit-ripening in the Loess Plateau region of China. To understand such phenomenon at post-transcriptional level, we have investigated the roles of microRNAs (miRNAs) in response to debagging. Three small RNA libraries were primarily constructed from peels of ‘Granny Smith’ apples subjected to bagging followed by sunlight re-exposure treatments (0h, 6h, 1d) (debagging), and from peels of apples without any bagging treatments (0h, 6h, 1d). 201 known miRNAs belonging to 43 miRNA families and 220 novel miRNAs were identified via high-throughput sequencing. Some miRNAs were found to be differentially expressed after debagging, which indicated that miRNAs affected anthocyanin accumulation through their target genes in mature apple. To further explore the effect of debagging on miRNAs regulating the expression of anthocyanin regulatory genes, four miRNAs and their target genes regulating anthocyanin accumulation, miR156, miR828, miR858 and miR5072, were compared between green cultivar ‘Granny Smith’ and red cultivar ‘Starkrimson’. Results showed that mdm-miR828 and mdm-miR858 regulated anthocyanin contents in both apple cultivars, while mdm-miR156 only affected anthocyanin accumulation in ‘Granny Smith’, and miR5072 affected anthocyanin accumulation in ‘Starkrimson’. Additional analysis of gene ontology for the differentially expressed miRNAs after debagging treatments and their predicted target genes showed that they were involved in photo-protective response after debagging from 0h to 1d; they might play important roles in fruit development and adaptation to high light stress
Untargeted metabolomics reveals dose-response characteristics for effect of rhubarb in a rat model of cholestasis
No full textCholestasis is a serious manifestation of liver diseases with limited therapies. Rhubarb, a widely used herbal medicine, has been frequently used at a relatively large dose for treating cholestasis. However, whether large doses are optimal and the therapeutic mechanism remain unclear. To explore these questions, the anti-cholestatic effect of five doses of rhubarb (0.21, 0.66, 2.10, 6.60, and 21.0 g/kg) in an alpha-naphthylisothiocyanate (ANIT)-induced rat model of cholestasis was examined by histopathology and serum biochemistry. A dose-dependent anti-cholestatic effect of rhubarb (0.21-6.6 g/kg) was observed, and an overdose of 21.0 g/kg showed a poor effect. LC-MS-based untargeted metabolomics together with pathway analysis were further applied to characterize the metabolic alterations induced by the different rhubarb doses. Altogether, 13 biomarkers were identified. The dose-response curve based on 9 important biomarkers indicated that doses in the 0.42-6.61 g/kg range (EC20-EC80 range, corresponding to 4.00-62.95 g in the clinic) were effective for cholestasis treatment. The pathway analysis showed that bile acid metabolism and excretion, inflammation and amino acid metabolism were altered by rhubarb in a dose-dependent manner and might be involved in the dose-response relationship and therapeutic mechanism of rhubarb for cholestasis treatment
