2,474 research outputs found
CHONDROITIN SULFATE AS A BIOACTIVE MACROMOLECULE FOR ADVANCED BIOLOGICAL APPLICATIONS AND THERAPIES
No full textGlycosaminoglycans (GAGs) are acidic, highly sulfated, complex, linear, natural heteropolysaccharides
distributed among all organisms and composed of a variable number
of repeating disaccharide units. Each disaccharide consists of one hexosamine, Dgalactosamine
(GalN) or D-glucosamine (GlcN), and one uronic acid, D-glucuronic
acid (GlcA) or L-iduronic acid (IdoA) or neutral hexose, D-galactose (Gal). According
to the type of the monosaccharide units and the glycosidic bonds between them,
GAGs can be divided into four main categories: (1) hyaluronic acid or hyaluronan
(HA); (2) chondroitin sulfate (CS) and dermatan sulfate (DS); (3) heparan sulfate
(HS) and heparin (Hep); and (4) keratan sulfate (KS) (Fig. 5.1). GAGs are present
in all animals and some of them like Hep, CS and DS are extracted from terrestrial
and marine soft tissues and cartilages for therapeutic uses. They are synthesized as
polymers of repeating disaccharides with an N-acetyl-hexosamine (GalNAc or Glc-
NAc) as one of the sugars. The alternating sugar is GlcA with the exception of KS
which contains galactose instead. HA, very peculiar because it is neither sulfated nor
covalently linked to a protein, containing GlcNAc, is not further modified, whereas
the other classes are modified by: (1) the addition of O-sulfate groups on various
hydroxyls (the three classes); (2) 5-epimerization of someGlcA residues to form IdoA
residues (DS, HS, Hep); and (3) removal of acetyl residues from some hexosamine
Decellularized matrices for tumor cell modeling
No full textCollagen is the main component of the extracellular matrix and it plays a key role in tumor progression. Commercial collagen solutions are derived from animals, such as rat-tail and bovine or porcine skin. Their cost is quite high and the product is stable only at low temperature, with the disadvantage of a short expiring date. Most importantly, lot-to-lot variability can occur and the reconstituted collagen gels differ significantly from native tissues in terms of both structure and stiffness. In this chapter, we describe a straightforward method to use native, collagen rich skin samples derived from by-products of the tanning industry. The protocol proposed preserves the microstructure of the ovine skin collagen network, offering structurally competent and more relevant model to investigate cell behavior in vitro. Other advantages of the proposed procedure consist in the cost-effectiveness of the process and an increased level of reproducibility. The decellularized ovine skin samples support the adhesion and growth of different cancer cell lines (pancreatic, breast and melanoma cells). The proposed decellularized skin scaffolds are meant as future low-cost competitors for conventional porous scaffold derived by biomaterials, since they offer a biomimetic environment for the cells
sj-pdf-1-ccx-10.1177_10732748211038736 – Supplemental Material for Impact of COVID-19 Pandemic on Cancer-Related Hospitalizations in Brazil
No full textSupplemental Material, sj-pdf-1-ccx-10.1177_10732748211038736 for Impact of COVID-19 Pandemic on Cancer-Related Hospitalizations in Brazil by Allini Mafra da Costa, Aline L. Ribeiro, Adeylson G. Ribeiro, Andrea Gini, Citadel Cabasag, Rui M. Reis, José Humberto T. G. Fregnani and Isabelle Soerjomataram in Cancer Control</p
Supplement_4 – Supplemental material for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review
No full textSupplemental material, Supplement_4 for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review by Renato Andrade, Joni Nunes, Betina B. Hinckel, Jordan Gruskay, Sebastiano Vasta, Ricardo Bastos, J. Miguel Oliveira, Rui L. Reis, Andreas H. Gomoll and João Espregueira-Mendes in CARTILAGE</p
Supplement_2 – Supplemental material for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review
No full textSupplemental material, Supplement_2 for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review by Renato Andrade, Joni Nunes, Betina B. Hinckel, Jordan Gruskay, Sebastiano Vasta, Ricardo Bastos, J. Miguel Oliveira, Rui L. Reis, Andreas H. Gomoll and João Espregueira-Mendes in CARTILAGE</p
Supplement_1 – Supplemental material for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review
No full textSupplemental material, Supplement_1 for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review by Renato Andrade, Joni Nunes, Betina B. Hinckel, Jordan Gruskay, Sebastiano Vasta, Ricardo Bastos, J. Miguel Oliveira, Rui L. Reis, Andreas H. Gomoll and João Espregueira-Mendes in CARTILAGE</p
Supplement_3 – Supplemental material for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review
No full textSupplemental material, Supplement_3 for Cartilage Restoration of Patellofemoral Lesions: A Systematic Review by Renato Andrade, Joni Nunes, Betina B. Hinckel, Jordan Gruskay, Sebastiano Vasta, Ricardo Bastos, J. Miguel Oliveira, Rui L. Reis, Andreas H. Gomoll and João Espregueira-Mendes in CARTILAGE</p
Market Integration in the Golden Periphery - the Lisbon/London Exchange, 1854-1891
Get PDFThe existence of a self-regulating arbitrage mechanism under the gold standard has been traditionally considered as one of its main advantages, and attracted a corresponding research interest. This research is arguably relevant not only to test for the efficiency of the “gold points”, but also to study the evolution of financial integration during the so-called first era of globalization. Our first aim with this paper is to contribute to the enlargement of the scope of the literature by considering the case of Portugal that adhered to the system, in 1854, at a much earlier phase than the majority of countries, thus allowing for a broader perspective on the evolution of the efficiency of the foreign exchange market. As a typical “peripheral” country, Portugal can be used as the starting point for a study of the degree of integration of the periphery within the system. Furthermore, the Portuguese exchange also illustrates the role in practice of large players in sustaining currency stability, over and beyond the atomistic forces of arbitrage and speculation assumed in conventional theoretical frameworks. We also address the question of the credibility of the authorities’ commitment to the standard, through the perspective of the target zone literature.
Optimizing the human perinatal progenitor cell secretome for future paracrine therapy to counteract cardiac disease with AmnioSMART: an ERA4Health-funded project
Get PDFThe EU-funded Partnership ‘Fostering a European Research Area for Health’ (ERA4Health) is a programme bringing together 33 institutions from different countries, including 17 out of the 27 members of the EU, and 5 Third Countries (from which 3 are associated to Horizon Europe). The main aim of the ERA4health is to regularly sponsor international joint calls for proposals aimed at developing breakthrough collaborative research joint strategies to address current unmet clinical needs and thus reduce disease burden across Europe (source: https:// era4health.eu/at-a-glance). Among the different funding opportunities, the 2023 ERA4Health framework focused on innovative, yet feasible, treatments for cardiovascular disease with specific translational potential, as per the main goal of the call: ‘Research targeting development of innovative therapeutic strategies in cardiovascular disease (CARDINNOV)’
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