1,278 research outputs found
Relative abundance of prokaryotes in sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats)
No full textCARD-FISH was performed as previously described in Ruff et al., (2013; doi:10.1371/journal.pone.0072627) with the following modifications. 4-6 µl of 25-fold diluted sediment were used for filtration. Archaeal cell walls were permeabilized with 0.1M HCl for 2 min to detect ANME-3 cells, or Proteinase K solution (15 µg ml-1 (Merck, Darmstadt, Germany) in 0.05 M EDTA (pH 8), 0.1 M Tris-HCl (pH 8), 0.5 M NaCl) for 2-4 min at room temperature for all other archaea. Bacterial cell walls were permeabilized with lysozyme solution (1000kU/ml) for 60 min at 37°. Cells were stained with DAPI (1µg/ml), embedded in mounting medium and counted in 40-60 independent microscopic fields using an Axiophot II epifluorescence microscope (Carl Zeiss, Jena, Germany)
Emil Wohlwill 1835-1912
No full textBiography of Emil Wohlwill, based mainly on his letters to his sister and wife; childhood and education in Hamburg; interest in literature and art; patriotism and engagement in "Hamburger Arbeitsverein"; work as a chemist; travel to Italy for research on Galileo book.Emil Wohlwill (Hamburg 1835 - 1912) was the son of Immanuel Wohlwill (ME 696). He was a chemist at the "Hamburger Affinerie" and president of the Hamburger Arbeitsverein. Wohlwill was also the author of a book on Galileo Galilei.Synopsis in fileEducation; primary and secondary; before 1871Hamburger Arbeitsverei
Carrie's letters to her Emil. Published for the author.
No full textEdited by Emil Klopfer.Mode of access: Internet.BANC; F855.K5: Bancroft Library also has positive microfilm.BANC; F855.K5: Master negative available (89-0183). Shelved with Main's negatives
Metadata und statistic analysis of archaeal and bacterial sequences originating from sediments of the Håkon Mosby mud volcano (all habitats)
No full textDNA extraction was carried out as described on the MICROBIS project pages (http://icomm.mbl.edu/microbis ) using a commercially available extraction kit. We amplified the hypervariable regions V4-V6 of archaeal and bacterial 16S rRNA genes using PCR and several sets of forward and reverse primers (http://vamps.mbl.edu/resources/primers.php). Massively parallel tag sequencing of the PCR products was carried out on a 454 Life Sciences GS FLX sequencer at Marine Biological Laboratory, Woods Hole, MA, following the same experimental conditions for all samples. Sequence reads were submitted to a rigorous quality control procedure based on mothur v30 (doi:10.1128/AEM.01541-09) including denoising of the flow grams using an algorithm based on PyroNoise (doi:10.1038/nmeth.1361), removal of PCR errors and a chimera check using uchime (doi:10.1093/bioinformatics/btr381). The reads were taxonomically assigned according to the SILVA taxonomy (SSURef v119, 07-2014; doi:10.1093/nar/gks1219) implemented in mothur and clustered at 98% ribosomal RNA gene V4-V6 sequence identity. V4-V6 amplicon sequence abundance tables were standardized to account for unequal sampling effort using 1000 (Archaea) and 2300 (Bacteria) randomly chosen sequences without replacement using mothur and then used to calculate inverse Simpson diversity indices and Chao1 richness (doi:10.2307/4615964). Bray-Curtis dissimilarities (doi:10.2307/1942268) between all samples were calculated and used for 2-dimensional non metric multidimensional scaling (NMDS) ordinations with 20 random starts (doi:10.1007/BF02289694). Stress values below 0.2 indicated that the multidimensional dataset was well represented by the 2D ordination. NMDS ordinations were compared and tested using Procrustes correlation analysis (doi:10.1007/BF02291478). All analyses were carried out with the R statistical environment and the packages vegan (available at: http://cran.r-project.org/package=vegan), labdsv (available at: http://cran.r-project.org/package=labdsv), as well as with custom R scripts. Operational taxonomic units at 98% sequence identity (OTU0.03) that occurred only once in the whole dataset were termed absolute single sequence OTUs (SSOabs; doi:10.1038/ismej.2011.132). OTU0.03 sequences that occurred only once in at least one sample, but may occur more often in other samples were termed relative single sequence OTUs (SSOrel). SSOrel are particularly interesting for community ecology, since they comprise rare organisms that might become abundant when conditions change.16S rRNA amplicons and metagenomic reads have been stored in the sequence read archive under SRA project accession number SRP042162
Editorial: microbial communities and metabolisms involved in the degradation of cellular and extracellular organic biopolymers
No full text© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ruff, S. E. Editorial: microbial communities and metabolisms involved in the degradation of cellular and extracellular organic biopolymers. Frontiers in Microbiology, 12, (2022): 802619, https://doi.org/10.3389/fmicb.2021.802619.Most organic matter on Earth occurs in the form of macromolecules and complex biopolymers, which include the building blocks of every organism. Plant, animal, fungal, and microbial cells largely consist of macromolecules belonging to four compound classes: proteins, polysaccharides, nucleic acids, and lipids (Figure 1). The percentage of these compounds per dry weight can vary greatly between lineages, but also between individuals of the same species or developmental stages of the same organism. Living and lysing cells release a substantial quantity and variety of macromolecules to the environment. These compounds often contain nitrogen, phosphorus, and sulfur, in addition to carbon, and are thus ideal food sources for heterotrophic organisms. Although the degradation of biopolymers and macromolecules has received considerable attention, many knowledge gaps remain, particularly in very complex ecosystems such as soils and sediments
Pioneer Finns and Swedes of Delaware Valley Before William Penn
No full textA brief history of Finnish and Swedish settlers in the Delaware River Valley, starting with the 17th century. By Emil Linclon Ostman
Emil Fahrenkamp: Bauten und Projekte für Berlin
No full textThema der vorliegenden Arbeit ist die umfassende Aufarbeitung und Dokumentation der Bauten und Projekte Emil Fahrenkamps (*1885, 1966) in Berlin und Potsdam-Babelsberg sowie die kritische Einordnung seiner Arbeiten in den Kontext der Baugeschichte des 20. Jahrhunderts. Den zentralen Untersuchungsgegenstand bildet, neben der gebaudespezifischen Erfassung der Bau- und Planungsprozesse und der Gebaudebeschreibung Fahrenkamps grosstadtische Arbeiten und Entwurfe in ihrem gesellschaftlichen Kontext zu analysieren. Zeitlich umfasst die Arbeitsphase Fahrenkamps in Berlin die Jahre zwischen 1920 und 1945, in denen er seine bauliche Aktivitaten sowohl fur private Bauherrn als auch fur offentliche Auftraggeber etablieren und ausbauen konnte. Emil Fahrenkamp galt bisher als ein Vertreter der Moderne in Deutschland. Dieses fest gefugte Bild entstand durch die einseitige Gleichsetzung seiner Architektur mit dem Shell-Haus, das in den Jahren 192932 nach seinen Planen in Berlin errichtet wurde und das zur Ikone grosstadtischen Bauens in der baugeschichtlichen Bewertung geworden ist. Die vorliegende Arbeit sucht diese Sicht auf Emil Fahrenkamp kritisch zu hinterfragen und zu differenzieren. Indem sie exemplarisch seine Arbeiten fur Berlin aufarbeitet, gelingt eine neue Sicht seiner architektonischen Intention und damit eine Neubewertung seiner Bedeutung in der deutschen Baugeschichte des 20. Jahrhunderts.Architectur
Emil Cioran: The transfiguration of Romania
No full textTitle: Schimbarea la faţă a României (The transfiguration of Romania) Originally published: Bucharest: Vremea, 1936 Language: Romanian The present excerpts are from Emil Cioran, Schimbarea la faţă a României (Bucharest: Humanitas, 1990), pp. 39–43, 59–60. About the author Emil Cioran [1911, Răşinari (Hun. Resinár; Ger. Städterdorf), (Transylvania) – 1995, Paris]: philosopher. His father was an Orthodox priest. After completing his secondary education in Sibiu in 1927, he went to Bucharest to ..
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