648 research outputs found
Lysophosphatidic acid induces ovarian cancer cell dispersal by activating Fyn kinase associated with p120-catenin
No full textJia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang
No full textWong, Chai Kwok = 加入聚合物對均勻湍流及均勻湍流對流的影響 / 黃濟國.Thesis M.Phil. Chinese University of Hong Kong 2013.Includes bibliographical references (leaves 89-91).Abstracts also in Chinese.Title from PDF title page (viewed on 01, November, 2016).Wong, Chai Kwok = Jia ru ju he wu dui jun yun tuan liu ji jun yun tuan liu dui liu de ying xiang / Huang Jiguo
APHERP symposium session II: Institutional management
No full textPresented Titles: Japan’s Responses to the Pandemic in Higher Education [Author: Futao Huang] Financing Higher Education in a Post-COVID Era [Author: Deane E. Neubauer] Who Influences Higher Education Decision-making in Taiwan? An Analysis of Internal Stakeholders [Authors: Sheng-ju Chan; Prudence Chuing Chou] COVID-19, Communities and Change [Author: Peter Duffy
Abstract 1820: Synergistic lethality of mAbs with an EMT reversal agent, Nintedanib, in epithelial ovarian cancer
No full textAbstract
Epithelial-mesenchymal transition (EMT) has long been associated with cancer progression and metastasis. As a reversible process, it has the potential to be a target for cancer therapy. However, this concept of reversing EMT has not yet been widely explored in current treatment strategies. With the identification of Nintedanib as an EMT reversal agent, we aim to broaden the utility of this triple angiokinase inhibitor by identifying agents that show synergistic lethality with it, using monoclonal antibodies (mAbs) as a combinatory therapeutic. Nintedanib alone is not significantly cytotoxic to cells, but it causes cell cycle arrest and reverses EMT. By selecting for this sub-population of cells that are sensitive to Nintedanib, their subsequent eradication can then be more intricately directed. Since the anti-cancer application of EMT reversal agents are not fully developed, it is vital to investigate mechanisms that sensitize cancer cells to cytotoxic agents following EMT reversal.Our group has generated panels of mAbs that are able to bind differentially to various breast and ovarian cancer cell lines along the EMT spectrum. Some mAbs preferentially bind to Epithelial lines, while some have stronger affinity to Mesenchymal lines. This implies that mAbs have the ability to differentiate between epithelial and mesenchymal phenotypes, uncovering a new dimension to the capability of mAbs which have yet to be explored.Using a mesenchymal ovarian cancer cell line as a starting model, Nintedanib-treated cells were screened with the mAbs and those showing a >2-fold increase in binding were shortlisted for further validation. Based on this criteria, 26 mAbs were identified and further characterized in functional Antibody Drug Conjugate (ADC) assays with mAbs conjugated to a toxic drug, Saporin (or Zap). Mabs successfully internalizing and killing the cancer cells will then be further chosen for antigen characterization and in vivo functional studies. Ultimately, there is huge potential for mAbs to be discovered that shows synergy with Nintedanib to enhance lethality to various types of cancer cells. A novel clinical strategy, the application of EMT reversal in terms of utilising a synthetic lethality-like approach allows better design of combinatory therapeutics, increasing treatment efficacy that might revolutionalise cancer treatment.
Citation Format: Jocelyn Teo, Heng Liang Tan, Ruby Yun-Ju Huang, Andre Choo. Synergistic lethality of mAbs with an EMT reversal agent, Nintedanib, in epithelial ovarian cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1820. doi:10.1158/1538-7445.AM2017-1820</jats:p
Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics
No full textThree-dimensional (3D) tissue culture platforms that are capable of mimicking in vivo microenvironments to replicate physiological conditions are vital tools in a wide range of cellular and clinical studies. Here, learning from the nature of cilia in lungs clearing mucus and pathogens from the airway - we develop a 3D culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for diminishing cell-to-substrate adhesion. Multicellular spheroids or colonies were tested for 3-7 days in a microenvironment consisting of generated cells with properties of putative cancer stem cells (CSCs). The dynamic and reversible regulation of epithelial mesenchymal transition (EMT) was examined in spheroids passaged and cultured in copolymer-coated dishes. The expression of CSC markers, including CD44, CD133, and ABCG2, and hypoxia signature, HIF-1 alpha, was significantly upregulated compared to that without the nano-cilia. In addition, these spheroids exhibited chemotherapeutic resistance in vitro and acquired enhanced metastatic propensity, as verified from microfluidic chemotaxis assay designed to replicate in vivo-like metastasis. The biomimetic nano-cilia approach and microfluidic device may offer new opportunities to establish a rapid and cost-effective platform for the study of anti-cancer therapeutics and CSCs. (C) 2013 Elsevier Ltd. All rights reserved
The CAM Model—Q&A with Experts
Get PDFThe chick chorioallantoic membrane (CAM), as an extraembryonic tissue layer generated by the fusion of the chorion with the vascularized allantoic membrane, is easily accessible for manipulation. Indeed, grafting tumor cells on the CAM lets xenografts/ovografts develop in a few days for further investigations. Thus, the CAM model represents an alternative test system that is a simple, fast, and low-cost tool to study tumor growth, drug response, or angiogenesis in vivo. Recently, a new era for the CAM model in immune-oncology-based drug discovery has been opened up. Although there are many advantages offering extraordinary and unique applications in cancer research, it has also disadvantages and limitations. This review will discuss the pros and cons with experts in the field
多元智慧取向的合作學習在國中籃球課程學習成效與態度之研究
No full text[[abstract]]The curriculum design of Multiple Intelligences Mode
And Cooperative learning in teaching of basketball in Junior high school
Researcher: Chu，Chiung-Fang
Advisor: Huang，Qing-Yun
Abstract
The purpose of this study is to investigate the learning achievement of corporate learning and traditional learning on basketball skill learning and students’ learning attitudes when these courses are based on the multiple intelligences proposed by Gardener. To achieve the purpose, this study apply an unequal group pretest and posttest design .A total of 136 samples are divided into two groups of which 69 are in the experimental group and the rest of 65 are in the control group. Before the experimental teaching, both groups are given pre-study evaluation on basketball skills and learning attitudes. Based on the pre-study evaluation, students in the experimental group are further heterogeneously divided into smaller teams with an average of five to six students in each team. The variant of this experiment is the teaching methods. Corporate learning method is used in the experimental group, while traditional teaching method is used in the control group. After four weeks of the learning experiment, posttest of learning achievements and attitudes of the students are immediately evaluated. The results indicate that:
1. The experimental group and the control group show significant improvement on their “30-second shoot.” (P<.05)
2. On the 30-second shoot, the average performance of the experimental group is slightly higher than that of the control group, but both fail to reach the significance level.
3. Both groups show considerable improvement on their dribble-jump stop- shooting and reach the significance level. (P<.05)
4. On the dribble-jump stop- shooting, the average performance of the control group is slightly higher than that of the experimental group, but both fail to reach significance level. (P<.05)
5. On the learning attitudes of the experimental group, the posttest is slightly higher than pretest but fail to reach the significance level.(P<.05)
6. The control group has great improvement in learning attitudes and also reaches the significance level. (P<.05)
In conclusion, we find that after the experimental teaching the group of cooperate and group of traditional learning performs both well. As to the learning attitudes, the group of traditional learning responses better than the cooperate learning.
Keyword:
multiple intelligences, corporate learning
UNCOVERING THE NOVEL ROLES OF AXL AND THE ASSOCIATED THERAPEUTIC VULNERABILITIES
No full textPh.DDOCTOR OF PHILOSOPHY (CSI
THE PIVOTAL ROLE OF AXL/GAS6 SIGNALING NODE IN THE MESENCHYMAL SUBTYPE OF OVERIAN CANCER, AND ITS MODULATION BY THE TUMOR SUPPRESSOR OPCML
No full textPh.DNUS-ICL JOINT PH.D. (NGS
ESTABLISHING AN EPITHELIAL MESENCHYMAL TRANSITION (EMT) SPECTRUM WITH EMT TRANSCRIPTIONAL DRIVERS
No full textPh.DPHD IN CANCER BIOLOG
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