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RuBi-GABA uncaging.
No full text(A) Top, plot of the normalized firing rate before during and after a 60 s uncaging pulse (blue bar) of 5 μM RuBi-GABA in the presence of 25 μM gabazine (n = 4). Bottom, plot of normalized firing rate (black line) and running standard deviation (grey area) before, during and after a 50 ms uncaging pulse in the presence of 5 μM RuBi-GABA and 25 μM gabazine (n = 12); application of 2 μM CGP 55845 blunted the changes in spiking induced by RuBi-GABA uncaging (orange line, n = 4). Example raster plots are shown at the top of the panel. (B) Left, two different time scales showing action potentials just prior to and after GABA uncaging. Right, overlaid action potentials from just prior to and after GABA uncaging showing a clear reduction in the mAHP. (C-D) As in panel A (bottom) and B, but in the absence of gabazine (n = 9).</p
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Photo of Mr. and Mrs. Frank Rubi (photograph)
No full textDelegates from Winslow, Arizona, Mr. and Mrs. Frank Rubi
Metode za detekciju Phytophthora fragariae var. rubi na malini
No full textPhytophthora fragariae var. rubi (Wilcox & Duncan), a causal agent of raspberry root rot, is
a serious soil-borne pathogen listed by EPPO as an A2 quarantine pest. Root samples were
collected from badly diseased raspberry plants showing a variety of characteristic and often
dramatic symptoms during surveys carried out in western Serbia in 2002. Identification of
the causal agent was performed in collaboration work with the Scottish Crop Research
Institute (S.C.R.I.), Dundee, UK. Necrotic roots were plated on selective French bean agar
(incorporating ampicilin, ryfamicin, bavistin and hymexasol). Detection of isolates was
based on cultural and morphological features compared with referent cultures.
DNA was extracted directly from the sampled roots using extraction buffer (200 mM
Tris- HCl pH 8.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS), purified by multi spin separation
columns [Thistle Scientific (Axygen)] or in 24:1 mixture of chlorophorm- iso-amyl alcohol
and amplified by nested PCR (ITS 4 and DC 6 for first round, DC 1 and DC 5 for second
round). Diluted DNA extracts were also amplified by conventional PCR with modified ”universal”
Phytophthora primers (ITS 6, ITS 7 and ITS 8, Cooke et al., 2000) and digested with
Msp1. Digestion patterns of the universal primers PCR product from infected roots matched
those of Scottish strains.
P. fragariae var. rubi occured on 8 out of 14 sites. Our results indicate that nested PCR (ITS
4 and DC 6 for first round, DC 1 and DC 5 for second round) or digestion of the ”universal”
Phytophthora primers PCR product for detection of P. fragariae var. rubi are more sensitive
and less time-consuming and therefore recommended for use.Phytophthora fragariae var. rubi (Wilcox & Duncan), prouzrokovač truleži korena maline,
je veoma značajan zemljišni patogen A2 karantinskog statusa u EPPO. Uzorci korena
su sakupljeni sa jako zaraženih biljaka maline koje su ispoljavale čitav niz karakterističnih
i često dramatičnih simptoma tokom pregleda koji su vršeni u zapadnoj Srbiji 2002. godine.
Identifikacija prouzrokovača je izvršena u saradnji sa Scottish Crop Research Institute
(S.C.R.I.), Dundee, UK. Nekrotični korenčići su unošeni u selektivnu French bean podlogu, koja
je sadržala ampicilin, rifamicin, bavistin i himeksazol. Detekcija dobijenih izolata je vršena
posmatranjem kulture i morfoloških karakteristika i poređenjem sa autentičnim kulturama.
DNK je ekstrahovana direktno iz uzorkovanih korenova pomoću ekstrakcionog pufera
(200 mM Tris-HCl pH 8,5, 250 mM NaCl, 25 mM EDTA, 0,5% SDS), prečišćena Multi spin separacionim
kolonama (Thistle Scientific (Axygen) ili 24:1 mešavinom hloroforma i izoamil
alkohola i amplifikovana nested PCR (ITS 4 i DC 6 za prvi krug, DC 1 i DC 5 za drugi krug).
Rastvoreni DNK ekstrakti su takođe amplifikovani putem konvencionalnog PCR sa modifikovanim
„univerzalnim” Phytophthora prajmerima (ITS 6, ITS 7 i ITS 8, Cooke i sar., 2000) i digestirani sa Msp1. Digestioni paterni proizvoda univerzalnih prajmera iz zaraženih korenova
su se poklapali sa škotskim izolatima.
P. fragariae var. rubi se javila na 8 od 14 lokaliteta. Naši rezultati ukazuju da su nested
PCR (ITS 4 i DC 6 za prvi krug, DC 1 i DC 5 za drugi krug) i digestija produkta univerzalnih
Phytophthora prajmera u cilju detekcije P. fragariae var. rubi senzitivnije i zahtevaju manje
vremena, pa ih stoga preporučujemo za korišćenje
Rubi
No full textSi presentano le iscrizioni latine, edite ed inedite, relative alla città di Rubi (Ruvo di Puglia), con introduzione storico-archeologic
Notices to the nomenclature of Asochyta rubi
No full textDie auf Rubus vorkommenden pathogenen Arten von Ascochyta werden kurz nomenklatorisch behandelt und Ascochyta rubi Sacc. neu benannt.Species of Ascochyta which are pathogen to Rubus are short discussed and Ascochyta rubi Sacc. is renamed into Ascochyta rubi-idaei
Rubi Dataset
No full textFrench productions (3) and metalinguistic reflections (2) written by Rubi (name is a pseudonym). See prompts for tasks in Dataset "Tasks Prompts"
Megachile rubi Mitchell, new comb.
No full textrubi Mitchell, new comb. Megachile rubi Mitchell 1924: 159. Female. Type locality: Raleigh, N.C. [U.S.A.]. Megachile neglecta Mitchell 1924: 160. Male. Type locality: U.S.A. Synonymy of Mitchell 1926 a: 117. Megachile (Xeromegachile) rubi Mitchell 1937 a: 362 364.Published as part of Raw, Anthony, 2002, New combinations and synonymies of leafcutter and mason bees of the Americas (Megachile, Hymenoptera, Megachilidae), pp. 1-43 in Zootaxa 71 on page 20, DOI: 10.5281/zenodo.15588
RUBI Literature Review
No full textLiterature Review on the Research Units in Behavioral Intervention (RUBI) Manua
Schoengastia rubi Vercammen-Grandjean 1958
No full text<i>Schoengastia rubi</i> Vercammen-Grandjean, 1958 <p> <i>Schoengastia rubi rubi</i> Vercammen-Grandjean, 1958b: 643, pls 5, 8.</p> <p> <i>Schoengastia (Schoengastia) rubi rubi</i> – Zumpt 1961: 158.</p> <p> <i>Schoengastia (Schoengastia) rubi</i> – Vercammen-Grandjean 1965c: 82.</p> Holotype <p>RMCA 113907.</p> Material revised <p>Holotype (not suitable for examination) and five paratypes from RMCA.</p> Distribution <p>DR Congo (Bukavu, Mushweshwe), Central African Republic (Soulemaka).</p> Hosts <p> <i>Acanthocercus atricollis</i>, <i>Naja melanoleuca</i>, <i>Numida meleagris</i>, <i>Trachylepis maculilabris</i>, <i>Passeriformes</i> gen. sp.</p>Published as part of <i>Stekolnikov, Alexandr A., 2018, Taxonomy and distribution of African chiggers (Acariformes, Trombiculidae), pp. 1-233 in European Journal of Taxonomy 395</i> on page 87, DOI: 10.5852/ejt.2018.395, <a href="http://zenodo.org/record/3829724">http://zenodo.org/record/3829724</a>
RUBI Literature Review
No full textLiterature Review on the Research Units in Behavioral Intervention (RUBI) Manua
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