1,362 research outputs found
Measuring protein reduction potentials using 15N HSQC NMR spectroscopy
Get PDFNMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple 15N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a
1H, 13C, 15N chemical shift assignments for oxidised ERp18
No full textCitation: Rowe, Michelle, L.; Ruddock, Lloyd, W.; Kelly, Geoff; Schmidt, Jürgen, M.; Williamson, Richard, A.; Howard, Mark, J.; "Solution Structure and Dynamics of ERp18: a Small ER Resident Oxidoreductase," Biochemistry 48(21), 4596-4606 (2009). PubMed: 19361226. DOI: 10.1021/bi9003342. System Studied: ERp18. System Components: ERp18, Polymer, 157 residues, 17771 Da. Molecular Description: Classification: Oxidoreductase, Structure Weight: 17801.10 Da, Molecule: Thioredoxin domain-containing protein 12, Polymer: 1, Type: polypeptide(L), Length: 157, Chains: A, EC#: 1.8.4.2, Fragment: UNP residues 24-172. Natural Source: Common Name: Human. Taxonomy ID: 9606. Superkingdom: Eukaryota. Kingdom: Metazoa. Genus/species: Homo sapiens. Source: Scientific Name: Homo sapiens, Common Name: Human, Expression System: Escherichia coli. Experimental Data: NMR parameters. Type: Assigned Chemical Shifts. Number of Values: 1760. Sets: 1
1H, 13C, 15N chemical shift assignments for reduced ERp18
No full textCitation: Rowe, Michelle, L.; Ruddock, Lloyd, W.; Kelly, Geoff; Schmidt, Jürgen, M.; Williamson, Richard, A.; Howard, Mark, J.; "Solution Structure and Dynamics of ERp18: a Small ER Resident Oxidoreductase," Biochemistry 48(21), 4596-4606 (2009). PubMed: 19361226. DOI: 10.1021/bi9003342. System Studied: ERp18. System Components: ERp18, Polymer, 157 residues, 17771 Da. Molecular Description: Classification: Oxidoreductase, Structure Weight: 17801.10 Da, Molecule: Thioredoxin domain-containing protein 12, Polymer: 1, Type: polypeptide(L), Length: 157, Chains: A, EC#: 1.8.4.2, Fragment: UNP residues 24-172. Natural Source: Common Name: Human. Taxonomy ID: 9606. Superkingdom: Eukaryota. Kingdom: Metazoa. Genus/species: Homo sapiens. Source: Scientific Name: Homo sapiens, Common Name: Human, Expression System: Escherichia coli. Experimental Data: NMR parameters. Type: Assigned Chemical Shifts. Number of Values: 601. Sets: 1
The Artistic Legacy of Georgia O’Keeffe
Get PDFThe work of American artist, Georgia O’Keeffe (1887-1986), was considered to be unique and revolutionary in her own time, but she ultimately achieved a prominent position in the history of art. The enduring inspiration of this important artist is demonstrated by an investigation of key artistic motifs as well as her legacy via the discussion of artists who have been influenced by her: Alfred Stieglitz, Arthur Dove, Marsden Hartley, and the author, Michelle L. Knutson
Teacher leaders as professional development providers: a case study
No full textHigh stakes assessments mandated by 2001 No Child Left Behind Act (NCLB) have increased the focus on student achievement causing a closer examination of the link between student achievement and teacher quality. Teacher training, through professional development, has been documented to be one of the most important factors in student achievement and may be one of the few indicators of student success that a school can control. As a result of a shift in beliefs and practices regarding professional development, current research has pointed in the direction of encouraging schools to embrace a professional development model led and supported by teacher leaders. This study’s purpose was to examine teacher leaders who receive their own professional development through a University Literacy Center, and what they do as professional development providers in their schools. The teacher leaders receive their own professional development from the Rutgers Center for Literacy Development. The specific research questions designed were: What do teacher leaders who receive professional development through a Literacy Center do as professional development providers once they return to their schools? What influences their activities? What are the supports and obstacles they face in doing so? The sample was comprised of three teacher leaders from a New Jersey District and their respective three principals. A case study was used to describe factors that influence the professional development that teacher leaders provided. Data collection sources were professional development session observations, teacher leader and principal interviews, and artifacts. Data from the interviews, professional development session observations, and examinations of relevant documents were used in order to discover emerging patterns. Data collection and analysis occurred simultaneously, as is the preferred and effective strategy in qualitative research design. The data indicated the following factors influencing the design and implementation of the professional development: teacher leader roles and internal resources, external resources, and relationships, along with the associated challenges. Used as a vehicle to transmit, facilitate and support ongoing teachers’ learning opportunities, teacher leaders can be necessary elements in the school’s organizational structure. Additionally, professional development organizations such as the Rutgers Center for Literacy Development can use this data to inform their offerings.Ed. D.Includes bibliographical referencesby Michelle L. Rose
Telecommunications policy in Turkey: restructuring for economic growth. European Policy Paper #11, November 2006
Get PDFIntroduction. Modern telecommunications technology is now widely seen as a critical driver in economic development. However, the issues involved in the rapid deployment of this technology are complex and frequently highly controversial. While some of these issues are technical, the most difficult ones involve changing a legal and regulatory framework which was originally designed for different times and different technologies. The process of changing this framework necessarily involves disruptive change for existing service providers as well as substantial benefits for the economy at large. This paper seeks to discuss these issues in light of Turkey’s progress to date in taking advantage of advanced available telecommunications technology and the myriad productivity-enhancing services that are associated with it.(1) An important element in developing a more competitive and dynamic sector in Turkey has been the desire of the country to become a member of the European Union. This has encouraged changes in the telecommunications regulatory regime following the guidelines set out in Chapter 19 of the EU “acquis” for candidate members. Nonetheless, substantial further efforts are needed to complete and implement the desired regulatory framework, particularly as it affects the former government monopoly carrier, Turk Telekom, and the cable companies. A further limiting factor has been an overall investment climate in Turkey which has been characterized by a high level of uncertainty for most investors, regardless of size or nationality. Policy recommendations to help accelerate the deployment of telecommunications technology include a clear reaffirmation of the government’s priorities for the sector, a reduction in the level of ownership and regulatory uncertainty, strengthening the Board and Staff of the Telecommunications Authority, and reviewing policies which reduce the scope and increase the cost of telecommunication licenses
Genetic influences on level and stability of self-esteem
Get PDFWe attempted to clarify the relation between self-esteem level (high vs. low) and perceived self-esteem stability (within-person variability) by using a behavioral genetics approach. We tested whether the same or independent genetic and environmental influences impact on level and stability. Adolescent twin siblings (n = 183 pairs) completed level and stability scales at two time points. Heritability for both was substantial. The remaining variance in each was attributable to non-shared environmental influences. Shared environmental influences were not significant. Level and stability of self-esteem shared common antecedents via genetic and non-shared environmental influences. Nonetheless, stability was influenced by substantial unique genetic and non-shared environmental influences. The results validate the notion that level and stability are partially autonomous components of self-esteem
The effects of extracellular matrix compliance and protein expression on neurons:
No full textExtracellular and intracellular cues affect neuronal morphology and contribute to brain diseases, such as schizophrenia, and injury. First, we examined how extracellular cues influence branching parameters of hippocampal neurons. Brain injury or disease can initiate changes in local or global stiffness of brain tissue. While stiffness of the extracellular environment is known to affect the morphology and function of many cell types, little is known about how the dendrites of neurons respond to changes in brain stiffness. We cultured hippocampal neurons on hydrogels composed of polyacrylamide of varying rigidities to mimic the effects of extracellular matrix stiffness on dendrite
morphology. At 12 days in vitro, dendrite branching was altered by stiffness; i.e. branching peaked in neurons grown on gels of intermediate stiffness (8 kPa). Furthermore, we found that ionotropic glutamate receptors play roles in regulating dendrite morphology. AMPA receptors play a role in dendritc arborization for harder stiffness, >2kPa, at all distances from the cell body. NMDA receptors play a role in dendritic arborization for a range of rigidities (1-25 kPa), at only proximal and intermediate distances from the cell body. However, a caveat to these studies is that cell adhesion is affected by the rigidity of these substrates. Addressing this caveat is of great importance because cell density affects dendrite branching. Thus, we also determined whether substrate stiffness plays a critical role in determining dendrite branching independent of cell density. We concluded that substrate stiffness does play a crucial role in determining dendrite branching patterns independent of cell number; however, the density of cells plated on substrates also influences the dendrite branching pattern of neurons. In the second chapter of my thesis, we looked at how intracellular proteins in different sections of the human brain are affected in schizophrenia. By Western blotting, we examined human, postmortem brain samples. NOS1AP protein expression increased in the dorsal lateral prefrontal cortex of patients with schizophrenia and not in the occipital lobe, medial temporal lobe, or cerebellum. Thus, this thesis demonstrates how extracellular and intracellular cues affect disease states, such as brain injury and schizophrenia.Ph.D.Includes bibliographical references (p. 101-119)by Michelle L. Previter
DNA fusion gene vaccination mobilizes effective anti-leukemic cytotoxic T lymphocytes from a tolerized repertoire
No full textThe majority of known human tumor-associated antigens derive from non-mutated self proteins. T cell tolerance, essential to prevent autoimmunity, must therefore be cautiously circumvented to generate cytotoxic T cell responses against these targets. Our strategy uses DNA fusion vaccines to activate high levels of peptide-specific CTL. Key foreign sequences from tetanus toxin activate tolerance-breaking CD4+ T cell help. Candidate MHC class Ibinding tumor peptide sequences are fused to the C terminus for optimal processing and presentation. To model performance against a leukemia-associated antigen in a tolerized setting, we constructed a fusion vaccine encoding an immunodominant CTL epitopederived from Friend murine leukemia virus gag protein (FMuLVgag) and vaccinated tolerant FMuLVgag-transgenic (gag-Tg) mice. Vaccination with the construct induced epitopespecificIFN-c-producing CD8+ T cells in normal and gag-Tg mice. The frequency and avidity of activated cells were reduced in gag-Tg mice, and no autoimmune injury resulted. However, these CD8+ T cells did exhibit gag-specific cytotoxicity in vitro and in vivo. Also, epitope-specific CTL killed FBL-3 leukemia cells expressing endogenous FMuLVgag antigen and protected against leukemia challenge in vivo. These results demonstrate a simple strategy to engage anti-microbial T cell help to activate epitope-specific polyclonal CD8+ T cell responses from a residual tolerized repertoire
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