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Differenze tra tessuti del frutto di una solanacea affine a melanzana a differenti tempi di maturazione attraverso misure di dinamica molecolare NMR
No full textStudies on the introduction of the maize Activator and Dissociation elements in transgenic chicory.
No full textGenetic engineering of parthenocarpic plants.
No full textTransgenic tobacco and eggplants expressing the coding region of the iaaM gene from Pseudomonas syringae pv. savastanoi, under the control of the regulatory sequences of the ovule-specific DefH9 gene from Antirrhinum majus, showed parthenocarpic fruit development. Expression of the DefH9-iaaM chimeric transgene occurs during flower development in both tobacco and eggplant. Seedless fruits were produced by emasculated flowers. When pollinated, the parthenocarpic plants produced fruits containing seeds. In eggplant, the genetic manipulation allowed fruit set and growth under environmental conditions prohibitive for fruit setting in the untransformed line, which did not set fruit at all. Under normal environmental conditions, production of marketable fruits took place from pollinated and unpollinated transgenic flowers, while flowers of untransformed control plants did produce fruits of marketable size only from fertilized flowers
Phospholipid fatty acids in anthers of eggplant (S.melogena), allied species and correspondent introgression lines
No full textLocation of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant
Full text link© Gramazio et al.; licensee BioMed Central. 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.NHM Repositor
Segregation distortion and linkage analysis in eggplant (Solanum melongena L.)
No full textAn anther-derived doubled haploid (DH) and an F2 mapping population were developed from an intraspecific hybrid between the eggplant breeding lines ‘305E40’ and ‘67/3’. The former incorporates an introgressed segment from S. aethiopicum gr. gilo carrying the Rfo-sa1 gene which confers resistance to Fusarium oxysporum; the latter is a selection from an intraspecific cross involving two conventional eggplant varieties and lacks Rfo-sa1.
Initially, 28 AFLP primer combination (PCs) were applied to a sample of 93 F2 individuals and 93 DH individuals, from which 170 polymorphic AFLP fragments were identified. In the DH population, the segregation of 117 of these AFLPs, as well as that of markers closely linked to Rfo-sa1 were substantially distorted, while in the F2 population, segregation distortion was restricted to just ten markers, thus the latter was chosen for map development.
A set of 141 F2 individuals was genotyped with 73 AFLP PCs (generating 406 informative markers), 32 SSRs, four tomato RFLPs and three CAPS markers linked to Rfo-sa1. This resulted in the assigning of 348 markers to 12 major linkage groups. The framework map covered 718.7 cM, comprising 238 markers (212 AFLPs, 22 SSRs, one RFLP and the Rfo-sa1 CAPS).
Marker order and inter-marker distances in this eggplant map were largely consistent with those reported in a recently published SSR-based map. From an eggplant breeding perspective, DH populations produced by anther culture appear to be subject to massive segregation distortion, and thus may not be very efficient in capturing the full range of genetic variation present in the parental lines
Introgression of resistance to Fusarium oxysporum f.sp. melongenae into cultivated eggplant and characterization of linked molecular markers for development of a markers assisted selection system
No full textTwo metallocarboxypeptidase inhibitors are implicated in tomato fruit development and regulated by the Inner No Outer transcription factor.
No full textThe TCMP-1 and TCMP-2 genes of tomato code for metallocarboxypeptidase inhibitors. TCMP-1 is highly expressed in flower buds before anthesis, while TCMP-2 in ripe fruits. To investigate their function, we altered their endogenous levels by generating transgenic plants harbouring a chimeric gene expressing the TCMP-1 coding sequence under the control of the TCMP-2 promoter. The expression of the transgene caused an earlier fruit setting with no visible phenotypic effects on plant and fruit growth. The altered TCMP-1 regulation determines an increased level of TCMP-1 in the fruit and unexpected changes in the levels of both TCMPs in flower buds before anthesis, suggesting a mechanism of transcriptional cross-regulation. We in silico analysed TCMPs promoter regions for the presence of common cis acting elements related to ovary/fruit development and we found that both promoters contain putative binding sites for INNER NO OUTER (INO), a transcription factor implicated in ovule development. By chromatin immunoprecipitation, we proved that INO binds to TCMP-1 and TCMP-2 promoters and represents a candidate regulatory factor for coordinated control of TCMPs
Development of RAPD-AFLP map of eggplant and improvement of tolerance to Verticillium wilt
No full textVerticillium wilt is one of the most serious diseases in eggplant and one of the main goals of eggplant breeding is the achievement of Verticillium wilt tolerant hybrids. Solanum sodomeum L. has shown a mechanism of partial resistance against Verticillium wilt. Interspecific hybrids were obtained by using an accession of S. sodomeum from Sicily and the eggplant variety Buia. The results of the screenings for eggplant genotypes tolerant/resistant to Verticillium wilt by using backcrosses are reported. In a naturally infected field, from 1998 to 2001, the resistance to V dahliae of backcrossed progenies was increased by about 60%. The interspecific hybrids were both selfed and backcrossed using different types of eggplant. An integrated linkage map of eggplant (Solanum melongena L.) has been obtained by using 48 plants of an F-2 population. Starting from a genetic RAPDs map with similar to 100 markers mapped on 13 linkage groups, 4 AFLPs primer combinations were screened in order to improve the genetic map with the aim to achieve markers linked to Verticillium tolerance. Mendelian segregation of loci was verified by chi-square tests of the expected 3:1 and 1:1 ratios. Marker order was determined and all the data were combined to construct the most likely map. The integrated analysis of markers resulted in the construction of a map consisting of 273 loci and 12/13 linkage groups spanning 736 cM in a total. Linkage between different AFLP markers and the tolerance to Verticillium is discussed
Hydropriming applied on fast germinating Solanum villosum Miller seeds: impact on pre-germinative metabolism
Full text linkSeed priming can circumvent poor germination rate and uniformity, frequently reported in eggplant (Solanum melongena L.) and its crop wild relatives (CWRs). However, there is still a gap of knowledge on how these treatments impact the pre-germinative metabolism in a genotype- and/or species-dependent manner. The CWR Solanum villosum Miller (hairy nightshade) investigated in this study showed a quite unique profile of fast germination. Although this accelerated germination profile would not apparently require further improvement, we wanted to test whether priming would still be able to impact the pre-germinative metabolism, eventually disclosing the predominant contribution of specific antioxidant components. Hydropriming followed by dry-back resulted in synchronized germination, as revealed by the lowest MGR (Mean Germination Rate) and U (Uncertainty) values, compared to unprimed seeds. No significant changes in ROS (reactive oxygen species) were observed throughout the treatment. Increased tocopherols levels were detected at 2 h of hydropriming whereas, overall, a low lipid peroxidation was evidenced by the malondialdehyde (MDA) assay. Hydropriming resulted in enhanced accumulation of the naturally occurring antioxidant phenolic compounds chlorogenic acid and iso-orientin, found in the dry seeds and ex novo accumulation of rutin. The dynamic changes of the pre-germinative metabolism induced by hydropriming are discussed in view of future applications that might boost the use of eggplant CWRs for breeding, upon upgrade mediated by seed technology
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