1,721,117 research outputs found
Dual mechanism of intercellular communication in HOBIT osteoblastic cells: a role for gap-junctional hemichannels.
No full textThe references of references: a method to enrich humanities library catalogs with citation data
No full textThe advent of large-scale citation indexes has greatly impacted the retrieval of scientific information in several domains of research. The humanities have largely remained outside of this shift, despite their increasing reliance on digital means for information seeking. Given that publications in the humanities have a longer than average life-span, mainly due to the importance of monographs for the field, this article proposes to use domain-specific reference monographs to bootstrap the enrichment of library catalogs with citation data. Reference monographs are works considered to be of particular importance in a research library setting, and likely to possess characteristic citation patterns. The article shows how to select a corpus of reference monographs, and proposes a pipeline to extract the network of publications they refer to. Results using a set of reference monographs in the domain of the history of Venice show that only 7% of extracted citations are made to publications already within the initial seed. Furthermore, the resulting citation network suggests the presence of a core set of works in the domain, cited more frequently than average
Infusione endovenosa protratta di Ceruleina nella pecora. Riscontri ultrastrutturali e valutazione statistica del numero di granuli di zimogeno in cellule pancreatiche
No full textThe references of references: Enriching library catalogs via domain-specific reference mining
No full textThe advent of large-scale citation services has greatly impacted the retrieval of scientific information for several domains of research. The Humanities have largely remained outside of this shift despite their increasing reliance on digital means for information seeking. Given that publications in the Humanities probably have a longer than average life-span, mainly due to the importance of monographs in the field, we propose to use domain-specific reference monographs to bootstrap the enrichment of library catalogs with citation data. We exemplify our approach using a corpus of reference monographs on the history of Venice and extracting the network of publications they refer to. Preliminary results show that on average only 7% of extracted references are made to publications already within such corpus, therefore suggesting that reference monographs are effective hubs for the retrieval of further resources within the domain
Question Answering-Based Query Expansion for Conversational Search: IIIA@UNIPD at TREC CAsT 2022
No full textMechanosensitivity and intercellular communication in HOBIT osteoblastic cells: a possible role for gap junction hemichannels
No full textThe impact of intra-operative factors in otosclerosis outcomes: retrospective study in a tertiary centre
Full text linkThe aim of the study was to assess results from a large cohort of patients undergoing otosclerosis surgery with respect to the impact of intra-operative variables on post-operative hearing function and complications. We enrolled 384 patients affected by otosclerosis who were subjected to stapes surgery between 2004 and 2013 at a single institution. Surgery was performed in all cases under local anaesthesia, using a manual perforator and/or microdrill. Teflon-piston prosthesis was used in all patients. Audiological data obtained preoperatively and at last follow-up examination (minimum 12 months) were compared. Statistical analysis was performed using the multiple regression model. Peripheral rim otosclerosis and diffuse otosclerosis were associated with better functional results compared to the obliterative pattern (p < 0.05). Mean post-operative Air-Bone Gap was significantly higher in the 0.4 mm, compared to 0.6 mm piston group at 0.5 kHz (p < 0.001) and 1 kHz (p < 0.02); in the stapedotomy group a statistically significant difference was found between 0.4 and 0.6 mm piston groups, in favour of the latter (p < 0.05). No differences were encountered in terms of average hearing threshold and complications. Intra-operative variables cannot be fully predictable and our data could help in stratification of the results and as a landmark for the surgeon’s decisions
Posttrannslational modification of bone collagen type I are related to the function of rat femoral regions
No full textH2O2 modulates purinergic-dependent calcium signalling in osteoblast-like cells
No full textReactive oxygen species (ROS) have long been considered as toxic by-products of aerobic metabolism and appear involved in the pathogenesis
of degenerative diseases. The physiological role of ROS as second messengers in cell signal transduction is, on the other hand,
increasingly recognized. Here we investigated the effects of H2O2 and extracellular nucleotides on calcium signalling in four osteoblastic cell
lines. In the highly differentiated HOBIT cells, sensitive to nanomolar concentrations of ADP and UTP, millimolar H2O2 induced oscillatory
increases of the cytosolic calcium concentration followed by a steady and sustained calcium increase. Long lasting rhythmic calcium activity
was induced by micromolar H2O2 doses. The H2O2-induced calcium signals, due to both release from intracellular stores and influx from the
extracellular milieu, were totally prevented by incubating the cells with the P2 receptor antagonist suramin or with the ATP/ADP hydrolyzing
enzyme apyrase. In the osteosarcoma SaOS-2 cells micromolar H2O2 failed to evoke calcium signals and millimolar H2O2 induced a slowly
developing calcium influx which was unaffected by suramin and apyrase. These cells responded to micromolar concentrations of ATP and
ADP, but were largely insensitive to UTP. ROS 17/2.8 osteosarcoma cells were totally insensitive to ATP, ADP and UTP in keeping with
the evidence that these cells lack functional purinergic receptors. In these cells, H2O2 up to 1mM did not increase the cytosolic calcium
concentration. In ROS/P2Y2 cells, stably expressing the P2Y2 receptor, spontaneous calcium oscillations were observed in 38% of the population
and nanomolar concentration of extracellular ATP or UTP activated oscillations in quiescent cells. Spontaneous calcium signals were
inhibited by suramin and apyrase. In these cells H2O2 induced oscillatory calcium activity that was blocked by suramin and apyrase. The
sensitivity of ROS/P2Y2 cells to UTP decreased significantly in the presence of DTT, which was effective also in inhibiting spontaneous
calcium oscillations. On the other hand, the membrane-impermeant thiol oxidant DTNB induced calcium oscillations that were inhibited
by incubating the cells with suramin or apyrase. Since peroxide did not increase extracellular ATP in these cell lines, we propose that, in
osteoblasts, mild oxidative conditions could activate purinergic signalling through the sensitization of P2Y2 receptor
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