2 research outputs found
Characterization of the immunological role of tissue factor in the endometrium of fertile women and of women with repeated abortions associated with antiphospholipid syndrome
No full textEl perfil endometrial leucocitario de mujeres con abortos a repetición (ARE) ha sido en los últimos años uno de los principales objetivos de investigación orientada al desarrollo de nuevas herramientas de diagnóstico y tratamiento. Más aún, estudios en marinos han demostrado que un aumento en la expresión de factor tisular (CD 142) en células inflamatorias ha sido implicado en la patogénesis del síndrome antifosfolipídico (SAF). Sin embargo, su expresión en pacientes con ARE no ha sido aún determinada. Se estudiaron 27 mujeres con ARE (SAF y no SAF) y 16 mujeres fértiles durante la ventana de implantación. Mediante una ecografía VOCAL doppler se obtuvieron los parámetros de vascularización endometrial. Se determinaron los niveles periféricos de linfocitos CD19+ CD5+ así como el recuento endometrial de células natural killer, neutrófilos y la expresión de factor tisular, IL6, VEGF y TNF-a. Pacientes con ARE presentaron un endometrio caracterizado por mayor expresión de factor tisular, mayor recuento endometrial de neutrófilos CD142+ , de natural killer y de linfocitos CD19+ CD5+ asociado a un aumento de la expresión de VEGF y TNF-a. Particularmente, pacientes con SAF presentaron un aumento de factor tisular que podría corresponderse con un mayor índice de vascularización en un contexto inflamatorio de mayor resistencia uterina.In recent years, endometrial leukocyte profile of women with recurrent abortions (RSA) has been one of the main objectives for the development of new diagnostic and treatment tools. Increased expression of tissue factor (CD 142) on inflammatory cells has been implicated in the pathogenesis of antiphospholipid antibodies (APA)-induced abortions in mice. However, its expression in RSA patients has not yet been determined. We studied 27 RSA patients (APA+ and APA- ) and 16 fertile women during window implantation. 3D endometrial VOCAL Doppler was performed. CD19+CD5+ cells were determined in peripheral blood. In addition, natural killer cells and neutrophils counts as well as tissue factor, IL-6, VEGF and TNF-a were measured in the endometrium. RSA patients expressed higher counts of endometrial tissue factor, neutrophil CD142+, natural killer cells and CD19+CD5+ lymphocytes associated with increased expression of VEGF and TNF-a. Particularly, APA+ patients exhibit increased expression of endometrial tissue factor which may correspond with a highest vascularization in an inflammatory context of increased uterine resistance.Fil: Incera, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Halitus Instituto Medico; ArgentinaFil: Ferrer, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Halitus Instituto Medico; ArgentinaFil: Mayol, SOledad. Inmunogénesis; ArgentinaFil: Vijoditz, Gustavo. Inmunogénesis; ArgentinaFil: Baldjian, Natalia. Inmunogénesis; ArgentinaFil: Garcia, Luciana. Centro de Investigación de la Fundación Repro; ArgentinaFil: Rodriguez Pazo, Eva. Halitus Instituto Medico; ArgentinaFil: Gutierrez, Gabriela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Inmunogénesis; Argentina. Centro de Investigación de la Fundación Repro; Argentin
Higher COVID-19 pneumonia risk associated with anti-IFN-α than with anti-IFN-ω auto-Abs in children
No full textWe found that 19 (10.4%) of 183 unvaccinated children hospitalized for COVID-19 pneumonia had autoantibodies (auto-Abs) neutralizing type I IFNs (IFN-alpha 2 in 10 patients: IFN-alpha 2 only in three, IFN-alpha 2 plus IFN-omega in five, and IFN-alpha 2, IFN-omega plus IFN-beta in two; IFN-omega only in nine patients). Seven children (3.8%) had Abs neutralizing at least 10 ng/ml of one IFN, whereas the other 12 (6.6%) had Abs neutralizing only 100 pg/ml. The auto-Abs neutralized both unglycosylated and glycosylated IFNs. We also detected auto-Abs neutralizing 100 pg/ml IFN-alpha 2 in 4 of 2,267 uninfected children (0.2%) and auto-Abs neutralizing IFN-omega in 45 children (2%). The odds ratios (ORs) for life-threatening COVID-19 pneumonia were, therefore, higher for auto-Abs neutralizing IFN-alpha 2 only (OR [95% CI] = 67.6 [5.7-9,196.6]) than for auto-Abs neutralizing IFN-. only (OR [95% CI] = 2.6 [1.2-5.3]). ORs were also higher for auto-Abs neutralizing high concentrations (OR [95% CI] = 12.9 [4.6-35.9]) than for those neutralizing low concentrations (OR [95% CI] = 5.5 [3.1-9.6]) of IFN-omega and/or IFN-alpha 2
