4,940 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
Inmunoterapia en pacientes con tumores de cabeza y cuello: Experiencia en nuestro centro
No full textRodriguez Alba, Laura; director de proyecto: Garrido García, Ana2023-2024Grado en MedicinaFacultad de Medicin
Segmentación Automática y Predicción de Respuesta Terapéutica en Cáncer de Mama: Un Enfoque Basado en Deep Learning sobre Datos Multicéntricos
No full textBernal Rodriguez, Alba; director de proyecto: Velasco Rebolledo, Jacinto2024-2025Grado en Ingeniería MatemáticaFacultad de Empresa y Tecnologí
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
Creación de una boutique para hombres en la ciudad de Esmeraldas
No full textCedeno Rodriguez, Alba Evelina: 'Plan de mejora en el proceso de cobro de Aranceles de la PUCESE en el período 2007 - 2008' . 142 p. Tesis Ingeniería en Contabilidad y AuditoriaDurante el desarrollo del presente proyecto se ha planteado metas para determinar si es factible o no la creación de una Boutique para hombre en la ciudad de Esmeraldas, aprovechando las oportunidades que el mercado ofrece y la falta de un tipo de tiendas en esta ciuda
Creación de una boutique para hombres en la ciudad de Esmeraldas
No full textCedeno Rodriguez, Alba Evelina: 'Plan de mejora en el proceso de cobro de Aranceles de la PUCESE en el período 2007 - 2008' . 142 p. Tesis Ingeniería en Contabilidad y AuditoriaDurante el desarrollo del presente proyecto se ha planteado metas para determinar si es factible o no la creación de una Boutique para hombre en la ciudad de Esmeraldas, aprovechando las oportunidades que el mercado ofrece y la falta de un tipo de tiendas en esta ciuda
Author Correction: Differential epigenetic reprogramming in response to specific endocrine therapies promotes cholesterol biosynthesis and cellular invasion
No full textThis Article omits a declaration from the Competing Interests statement, which should have included the following: ‘One of the authors, Y.L., is an editor on the staff of Nature Communications, but was not in any way involved in the journal review process. The other authors declare no competing interests.’ Also, the Article contains an error in the name of the author Alba Rodriguez-Meira, which is incorrectly given as Alba Meira. These errors have not been corrected in either the PDF or HTML versions of the Article. © 2019, The Author(s)
The Think Aloud Method for the Validation of Program Evaluation Instruments with English and Spanish Speaking Youth
No full textRodriguez, Michael C.; Pabon, Maria; Tafur, David A.; Alba-Johnson, Nydia. (2007). The Think Aloud Method for the Validation of Program Evaluation Instruments with English and Spanish Speaking Youth. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/195196
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
No full textNeltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Verga, Anibal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Rioja. Agencia de Extensión Rural La Rioja; ArgentinaFil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentin
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