142 research outputs found
Transport Functions of Ectoderm Epithelial Cells Forming Dental Enamel
No full textChapter 11. Transport Functions of Ectoderm Epithelial Cells Forming Dental Enamel Michael L. Paine, Alan Boyde, and Rodrigo S. Lacruz
Abstract:The development of enamel encapsulates fundamental cellular processes associated with ...
Mitochondria modulate ameloblast Ca 2+ signaling
No full textThe role of mitochondria in enamel, the most mineralized tissue in the body, is poorly defined. Enamel is formed by ameloblast cells in two main sequential stages known as secretory and maturation. Defining the physiological features of each stage is essential to understand mineralization. Here, we analyzed functional features of mitochondria in rat primary secretory and maturation stage ameloblasts focusing on their role in Ca(2+) signaling. Quantification of Ca(2+) stored in mitochondria by FCCP stimulation was comparable in both stages. The release of endoplasmic reticulum Ca(2+) pools by ATP in rhod2AM loaded cells showed similar (m)Ca(2+) uptake. However, (m)Ca(2+) extrusion via NCLX was more prominent in maturation. To address if (m)Ca(2+) uptake via the mitochondrial Ca(2+) uniporter (MCU) played a role in (c)Ca(2+) buffering, we stimulated Ca(2+) influx via the store operated Ca(2+) entry (SOCE) and blocked MCU with the inhibitor Ru265. This inhibitor was first tested using the enamel cell line LS8 cells. Ru265 prevented (c)Ca(2+) clearance in permeabilized LS8 cells like ruthenium red, and it did not affect ΔΨm in intact cells. In primary ameloblasts, SOCE stimulation elicited a significantly higher (m)Ca(2+) uptake in maturation ameloblasts. The uptake of Ca(2+) into the mitochondria was dramatically decreased in the presence of Ru265. Combined, these results suggest an increased mitochondrial Ca(2+) handling in maturation but only upon stimulation of Ca(2+) influx via SOCE. These functional studies provide insights not only on the role of mitochondria in ameloblast Ca(2+) physiology, but also advances the concept that SOCE and (m)Ca(2+) uptake are complementary processes in biological mineralization
Peer review report for: The efficiency of railways specialized in transporting iron ore and pellets
No full textPEER REVIEW REPORT FOR:
Fontan, R. G. de O., Rosa, R. A., & Lacruz, A. J. (2022). The efficiency of railways specialized in transporting iron ore and pellets. Revista de Administração Contemporânea, 26(1), e200284. https://doi.org/10.1590/1982-7849rac2022200284.en
HOW TO CITE THIS PEER REVIEW REPORT:
Fontan, R. G. de O., Rosa, R. A., Lacruz, A. J., & Marins, F. A. S. (2021). Peer review report for: The efficiency of railways specialized in transporting iron ore and pellets. RAC. Revista de Administração Contemporânea. Zenodo.
https://doi.org/10.5281/zenodo.5071313
REVIEWERS:
Fernando Augusto Silva Marins (Universidade Estadual Paulista, Brazil
High-Throughput Microscopy Analysis of Mitochondrial Membrane Potential in 2D and 3D Models
No full textRecent proteomic, metabolomic, and transcriptomic studies have highlighted a connection between changes in mitochondria physiology and cellular pathophysiological mechanisms. Secondary assays to assess the function of these organelles appear fundamental to validate these -omics findings. Although mitochondrial membrane potential is widely recognized as an indicator of mitochondrial activity, high-content imaging-based approaches coupled to multiparametric to measure it have not been established yet. In this paper, we describe a methodology for the unbiased high-throughput quantification of mitochondrial membrane potential in vitro, which is suitable for 2D to 3D models. We successfully used our method to analyze mitochondrial membrane potential in monolayers of human fibroblasts, neural stem cells, spheroids, and isolated muscle fibers. Moreover, by combining automated image analysis and machine learning, we were able to discriminate melanoma cells from macrophages in co-culture and to analyze the subpopulations separately. Our data demonstrated that our method is a widely applicable strategy for large-scale profiling of mitochondrial activity
La regulación de la vencidad civil derivada de la ley 11/1990, de 15 octubre, sobre reforma del Código Civil en aplicación del principio de no discriminación por razón de sexo, ...
No full textCopia digital. Zaragoza, 200
Género y educación, comentario al ensayo de Acker
No full textThis article is a commentary of the book: Acker S. (1995). "Gender and Education, Sociological Reflexions about women, education and feminism". Madrid: Nancea. Three questions are analyzed related to the sociology of education and gender impact: theories and feminist analysis, research about teacher women and professor women. This essay provides a lot of empiric indicators and results about the author studies. Her gender perspective is characterized by her openness and comprehensiveness.Este artículo constituye una referencia bibliográfica del siguiente texto: Acker S. (1995). "Género y Educación: Reflexiones sociológicas sobre mujeres, enseñanza y feminismo". Madrid: Nancea. Se abordan tres grandes cuestiones relativas a la sociología de la educación y la importancia de la variable género: las teorías y análisis feministas, los estudios sobre mujeres maestras en la escuela y sobre profesoras en las instituciones de educación superior. El ensayo aporta numerosos indicadores empíricos y resultados de las investigaciones realizadas por la autora desde una perspectiva de género amplia e integradora
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