197,191 research outputs found
Metabolomics dataset of 44 Propionibacterium freudenreichii for Scoary2
No full text44 yogurts were produced, each by combining a different (sequenced) Propionibacterium freudenreichii strain with the same starter culture (Yoflex® YC-381). The metabolomes of these yogurts were measured using liquid chromatography MS (LC-MS) and gas chromatography MS (GC-MS), the latter measuring the yogurt’s volatile metabolites (volatiles). The aim of this study was to investigate the effect of the pan-genome of the added bacterial strains on the phenotype of the yogurts. We discovered two genes affecting carnitine metabolism.This data was analyzed using Scoary2: https://github.com/MrTomRod/scoary-2/wiki/Output#example-outputFile descriptions:- traits_44_noraw.tsv: corrected and filtered metabolomics data. Includes lc-ms as well as gc-ms volatiles data- traits_info_44_noraw.tsv: metadata about the measured metabolites, e.g. m/z, retention times, descriptions, putative HMDB IDs, etc.- isolate_info_44.tsv: links each sample to its species, in this case, all samples are Propionibacterium freudenreichii.- N0.tsv: a list of Hierarchical Orthogroups (HOGs) that groups orthologous genes together. It was created using OrthoFinder v2.5.4 based on protein FASTA files.- N0_best_names.tsv: maps each HOG to a putative descriptio
8. Brochetta C., Perrotta M.G., Jeromin A., Romano M., Soranzo MR., Borelli V., Roder J. And Zabucchi G. Identification and Subcellular Localization of Neurtonal Calcium Sensor-1 (NCS-1) in Human Neutrophils and HL-60 cells.
No full textDynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopy
No full textWe report single-molecule measurements on the folding and unfolding conformational equilibrium distributions and dynamics of a disulfide crosslinked version of the two-stranded coiled coil from GCN4. The peptide has a fluorescent donor and acceptor at the N termini of its two chains and a Cys disulfide near its C terminus. Thus, folding brings the two N termini of the two chains close together, resulting in an enhancement of fluorescent resonant energy transfer. End-to-end distance distributions have thus been characterized under conditions where the peptide is nearly fully folded (0 M urea), unfolded (7.4 M urea), and in dynamic exchange between folded and unfolded states (3.0 M urea). The distributions have been compared for the peptide freely diffusing in solution and deposited onto aminopropyl silanized glass. As the urea concentration is increased, the mean end-to-end distance shifts to longer distances both in free solution and on the modified surface. The widths of these distributions indicate that the molecules are undergoing millisecond conformational fluctuations. Under all three conditions, these fluctuations gave nonexponential correlations on 1- to 100-ms time scale. A component of the correlation decay that was sensitive to the concentration of urea corresponded to that measured by bulk relaxation kinetics. Thetrajectories provided effective intramolecular diffusion coefficients as a function of the end-to-end distances for the folded and unfolded states. Single-molecule folding studies provide information concerning the distributions of conformational states in the folded, unfolded, and dynamically interconverting states.Author manuscript. Published in final edited form as: Proc Natl Acad Sci U S A. 2000 November 21; 97(24): 13021-13026.The final published version of this article is located at: http://www.pnas.org/cgi/reprint/97/24/13021NIH GM54616; to William F. DeGradoNIH GM12592; to Robin M. HochstrasserNIH GM48130; to William F. Degrado and Robin M. HochstrasserThis work was supported by GM54616 (to W.F.D.), GM12592 (to R.M.H.) and GM48130 (to W.F.D. and R.M.H.) with instrumentation developed under RR01348. D.S.T. was supported by National Institutes of Health Grant NRSA F32-GM18589.Also available in PubMed Central. PMCID:PMC2717
Risk perception of local stakeholders on natural hazards: implications for theory and practice
No full textIn Romania, local stakeholders' knowledge plays a role in making decisions for emergencies, supporting rescue officers in natural hazard events, coordinating, and assisting the affected populations physically and psychologically. However, despite the increasing occurrence and severity of natural hazards in the IaÈTMi metropolitan area (of north-eastern Romania), there is a lack of knowledge of local stakeholders on how to encourage the population regarding safety actions. For this reason, we interviewed 118 local stakeholders to determine their risk awareness and preparedness capacities over a set of natural hazards, in order to understand where deficiencies in knowledge, action, and trust are greatest. Results reveal substantial distinctions between different threats and among stakeholders based on their cognitive and behavioural roles in the communities. The roles of responsibility and trust are important driving factors shaping their perception and preparedness. Preparedness levels are low, and, for many, learning and preparatory actions are needed to build resilience to the negative occurrences of natural hazards. As the stakeholders' role is to direct interventions in affected areas by managing communication initiatives with the entire population of the community, there is a need to create stakeholders' networks, empowering local actors and serving as a bridge between authorities' decisions and local people, making effective risk management plans, and securing more lives and economies
CLT: programma strutturato per la riabilitazione del paziente schizofrenico nelle arre Casa, Lavoro e Tempo libero
No full textDr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
Factores bióticos relacionados con el decaimiento de Pinus pinaster en Castilla y León
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