1,625 research outputs found
Ghrelin-mediated pathway in Apolipoprotein-E deficient mice: a survival system
No full textRenal diseases interfere with the regulation of several metabolic pathways including dyslipidemia. The latter includes increased triglycerides, very low-density lipoprotein levels and decreased high-density lipoproteins. These lipoproteins change during renal injury. Apolipoprotein-E deficient mice (ApoE-/-) are considered a very well accepted model of hypercholesterolemia with marked renal pathological alterations. Ghrelin hormone is mainly secreted from the stomach when the stomach is empty, but it is also found in the kidney. In this organ it has autocrine and/or paracrine roles determining glomerular filtration rate, tubular phosphate and sodium reabsorp- tion. Interestingly, it has been demonstrated that ghrelin levels increase after fasting. This mechanism induces an interaction with sirtuin 1 (SIRT1)/p53 pathway suggesting a link between ghrelin and SIRT1 in the regulation of salt and water metabolism. The mechanisms of ghrelin-induced SIRT1 expression are not yet fully understood. Recent studies indicate that SIRT1 exerts renoprotective properties against kidney diseases. This could be a very interesting point for underlining the important role of the ghrelin-SIRT1 system. Water movement across biological cell membranes is enhanced or facilitated by tetrameric membrane-bound channels, named aquaporin (AQP) fam- ily, and in particular, AQP1 and AQP2 proteins. In this study, we evaluated the possible pathway existing among the ghrelin/SIRT1/AQP1/AQP2 system in APOE-/- mice in order to clarify or stress the role played by said system in renal diseases associated to aging with or without comorbities. The results could provide a basis for considering ghrelin as a new target for therapeutic strategies of renal injury
3D printing of biocompatible polymers for personalized skull-base reconstruction: proof of concept
No full textPlatelet preparations in neuronal cell differentiation.
No full textConcentrated Growth Factors (CGF) is a platelet rich preparation that has the
important feature of a tight fibrin network and containing a large number of growth
factors possessing great regenerative potentialities [1].
The regeneration of nervous system is one of the mail goal of regenerative medicine.
The aim of this study is to test the in vitro CGF effects on both differentiated and
undifferentiated SH-SY5Y cells, derived from human neuroblastoma.
To induce differentiation, SH-SY5Y cells have been treated with Retinoic Acid
(RA) 10μM, in both basal and complete medium and in the presence and absence
of CGF. After 72 hours, different parameters have been investigated: the morphological
characteristics of the cells, the cell proliferation, the cellular vitality using the
MTT test, the CGF and/or RA differentiation property and the immunocytochemical
analysis of neuronal specific markers (NeuN, Sinaptophisine, β-III-tubulin, Nestin).
Moreover the NGF (Nerve Growth Factor) and BDNF (Brain Derived Growth Factor)
release have been assayed by ELISA test.
Our results obtained suggest that treatment with CGF, also used alone, positively
affects cell differentiation and neuronal phenotype regulating the expression of the
neuronal markers and improving the outgrowth of neurites.
Taken together these results seems to be promised into new approaches for neuronal
regeneration using platelet preparations
VCC-LF dataset
No full textThis is readme for VCC-LF dataset.
This dataset provides light field mat files that capture by Lytro I.
The light field resolusion is [h,w,u,v,d].
If you use these data or our toolkit code, please cite our paper properly
@inproceedings{ lirsiggraphasia2019,
title={Hierarchical and View-invariant Light Field Segmentation by Maximizing Entropy Rate on 4D Ray Graphs},
author={Li, Rui and Heidrich, Wolfgang},
booktitle={ACM Transactions on Graphics (Proc. SIGGRAPH Asia)},
year={2019},
publisher={ACM}
LF-copying without LF
No full textAbstractA copying approach to ellipsis is presented, whereby the locus of copying is not a level of derived syntactic structure (LF), but rather the derivation itself. The ban on preposition stranding in sprouting follows without further stipulation, and other, seemingly structure sensitive, empirical generalizations about elliptical constructions, including the preposition stranding generalization, follow naturally as well. Destructive operations which ‘repair’ non-identical antecedents are recast in terms of exact identity of derivations with parameters. In the context of a compositional semantic interpretation scheme, the derivational copying approach to ellipsis presented here is revealed to be a particular instance of a proform theory, thus showing that the distinctions between, and arguments about, syntactic and semantic theories of ellipsis need to be revisited
Intraepidermal Injections of Autologous Epidermal Cell Suspension: A new promising approach to Dermatological Disorders. Preliminary Study
No full textRegenerative medicine is a modern approach of dermatological treatment, using Epidermal Cells of the interfollicular epidermis (ESCs) for their effect in skin regeneration in chronic ulcers and burns, melanoma, vitiligo, junctional epidermolysis bullosa. Intraepidermal injections of autologous epidermal cell suspension can be a new and very promising treatment for many other cutaneous disorders as non-scarring alopecia (Alopecia
Areata, Androgenic Alopecia) or scarring alopecia (Lichern Plano Pilaris alopecia, Discoid Lupus Erithematosus alopecia), anti-aging therapies. The intraepidermal injection of an autologous epidermal cell suspension is a simple, fast and safe surgical procedure: a small, thin portion of the epidermis of the patient undergoes a treatment where a suspension with all the cells collected from the epidermis and cultured for 7 days is injected
into the skin. Our preliminary study shows that a suspension contains a signifi cant number of viable cells that survive at day 7 in culture
AQP1 expression in human gingiva and its correlation with periodontal and peri-implant tissue alterations.
No full textAquaporins (AQPs) are a family of hydrophobic integral membrane proteins that function as transmem- brane channels and play an important role in tissue homeostasis. Aquaporin-1 (AQP1), in particular, has been reported to be involved in several biological processes including inflammation, angiogenesis, wound healing and others. Periodontitis and peri-implantitis can be defined as inflammatory processes that affect the tissues surrounding a tooth or an osseointegrated implant, respectively. To date, there are limited data about the involvement of AQPs in these diseases. The aim of this study was to evalu- ate the possible link between the histomorphological alterations and the expression of AQP1 in healthy, pathological and healed periodontal and peri-implant gingival tissues. The results obtained showed that changes in organization of collagen fibers were observed in periodontitis and peri-implantitis, together with an increase in the percentage of area occupied by inflammatory cell infiltration and an increase of AQP1 immunostaining, which was located in the endothelial cells of the vessels within the lamina propria. Moreover, in healed periodontal and peri-implant mucosa a restoration of histomorphological alterations was observed together with a concomitant decrease of AQP1 immunostaining. These data suggested a possible link between the degree of inflammatory state and the presence of AQP1, where the latter could be involved in the chain of inflammatory reactions triggered at periodontal and peri-implant levels
Reduction of precocious peri-implant resorption cone
Get PDFAim: After implant-insertion, bone tissue, newly-formed on peri-implant crest, undergoes to a mild marginal osseous readjustment due to build-up of inflammatory cell tissue (ICT). The present study verifies the possibility to limit bone resorption by placing implant fixtures 0.5 mm outside cortical bone edge. Methods: A clinically-controlled randomized study on 100 implants has been performed to compare early resorption process of implant fixtures placed 0.5 mm outside cortical bone edge with implant-fixtures inserted according to juxtacortical bone conventional protocols. Results: After 6 months, bone implant level was higher with emersion approach (-1.01±0.54 mm, mean±SD) than with submerged treatment (-1.56±0.5 mm) (P<0.001). Conclusion: Factors to achieve an excellent result at mean-long term seem to be very good, even though the latter have to be confirmed by follow-up
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