744 research outputs found
Antisense therapy: a potential breakthrough in the treatment of neurodegenerative diseases
No full text: Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration of neurons in the central or peripheral nervous system. Currently, there is no cure for neurodegenerative diseases and this means a heavy burden for patients and the health system worldwide. Therefore, it is necessary to find new therapeutic approaches, and antisense therapies offer this possibility, having the great advantage of not modifying cellular genome and potentially being safer. Many preclinical and clinical studies aim to test the safety and effectiveness of antisense therapies in the treatment of neurodegenerative diseases. The objective of this review is to summarize the recent advances in the development of these new technologies to treat the most common neurodegenerative diseases, with a focus on those antisense therapies that have already received the approval of the U.S. Food and Drug Administration
«PUTTING ARCHITECTURE ON A BUSINESS BASIS»: ALBERT KAHN AND THE SCIENTIFIC MANAGEMENT OF DESIGN WORK
No full textIn 1909, after having projected the first reinforced concrete factory in Detroit (Packard Building n. 10), Albert Kahn (1869-1942) is introduced to Henry Ford (1863-1947). From the joint work of those two self-made-men, an industrial capitalist and a promising architect, derives a revolutionary way of considering mass production and industrial architecture. The text examines the most innovative aspects of Albert Kahn work, that are the organization of designing following the hints given by Henry Ford in his factory organization. Federico Bucci, author of the book Albert Kahn: Architect of Ford (Princeton Architectural Press, New York 1994), inquires into the methods of work organization applied in the Albert Kahn Inc., that acquired enormous importance during the II World War climate
The Type III Intermediate Filament Protein Peripherin Regulates Lysosomal Degradation Activity and Autophagy
No full textPeripherin belongs to heterogeneous class III of intermediate filaments, and it is the only intermediate filament protein selectively expressed in the neurons of the peripheral nervous system. It has been previously discovered that peripherin interacts with proteins important for the endo-lysosomal system and for the transport to late endosomes and lysosomes, such as RAB7A and AP-3, although little is known about its role in the endocytic pathway. Here, we show that peripherin silencing affects lysosomal abundance but also positioning, causing the redistribution of lysosomes from the perinuclear area to the cell periphery. Moreover, peripherin silencing affects lysosomal activity, inhibiting EGFR degradation and the degradation of a fluorogenic substrate for proteases. Furthermore, we demonstrate that peripherin silencing affects lysosomal biogenesis by reducing the TFEB and TFE3 contents. Finally, in peripherin-depleted cells, the autophagic flux is strongly inhibited. Therefore, these data indicate that peripherin has an important role in regulating lysosomal biogenesis, and positioning and functions of lysosomes, affecting both the endocytic and autophagic pathways. Considering that peripherin is the most abundant intermediate filament protein of peripheral neurons, its dysregulation, affecting its functions, could be involved in the onset of several neurodegenerative diseases of the peripheral nervous system characterized by alterations in the endocytic and/or autophagic pathways
Role of the Intermediate Filament Protein Peripherin in Health and Disease
No full textIntermediate filaments are the most heterogeneous class among cytoskeletal elements. While some of them have been well-characterized, little is known about peripherin. Peripherin is a class III intermediate filament protein with a specific expression in the peripheral nervous system. Epigenetic modifications are involved in this cell-type-specific expression. Peripherin has important roles in neurite outgrowth and stability, axonal transport, and axonal myelination. Moreover, peripherin interacts with proteins involved in vesicular trafficking, signal transduction, DNA/RNA processing, protein folding, and mitochondrial metabolism, suggesting a role in all these processes. This review collects information regarding peripherin gene regulation, post-translational modifications, and functions and its involvement in the onset of a number of diseases
Itinerari dusiani nel teatro di Elena Bucci. Intorno a Non sentire il male
No full textL’intervista a Elena Bucci ripercorre gli itinerari dusiani e le tappe creative che hanno accompagnato la
scrittura scenica di Non sentire il male, spettacolo
del 2000 dedicato a Eleonora Duse, di cui Bucci è autrice, attrice e regista. L’indissolubile intreccio di vita
professionale, artistica e privata della Grande Attrice
italiana viene qui affrontato attraverso una personale tessitura di documenti e fatti storici, che Bucci
passa nel filtro di un esplicito riconoscimento autobiografico: dalla sua formazione artistica alla fondazione della compagnia Le belle bandiere passando
per il Teatro di Leo, tappe in cui la figura “madrina”
di Duse è sempre evocata. Nel corso dell’intervista si
ricostruiscono inoltre i momenti salienti di ideazione
e sviluppo dello spettacolo, non solo in relazione alla
drammaturgia ma anche rispetto all’allestimento e
alle sue differenti versioni (site-specific, per la sala
teatrale, in forma di concerto). Accompagnano l’intervista alcuni apparati di contesto.The interview with Elena Bucci retraces dusian itineraries and the creative phases that traced the scenic
writing of Non sentire il male (2000), performance
dedicated to Eleonora Duse, whose Bucci is the author, actress and director. The indissoluble connection of the professional, artistic and private life of the
Great Italian Actress is processed here through a personal weaving of documents and historical facts,
which Bucci passes through the filter of an explicit
autobiographical transfer: from her artistic training
to the foundation of the company Le belle bandiere
through the Teatro di Leo, moments in which the figure of Duse is always invoked like a “godmother”.
During the interview, the salient passages of the conception and development of the performance are
also reconstructed, not only in relation to the dramaturgy but also with respect to the staging and its
different versions (site-specific, for black box, like a
live concert). Some contextual notes support the interview
Effectiveness of a Modified Administration Protocol for the Medical Treatment of Feline Pyometra
No full textPyometra is a common uterine disease of dogs and cats, typical of the luteal phase. Traditionally, ovariohysterectomy was considered the elective treatment for pyometra, but in some cases, such as breeding subjects or patients with a high anesthesiologic risk, medical treatment is preferred. Aglepristone is a progesterone receptor blocker and its use proved to be effective for the medical treatment of pyometra in bitches and queens. The aim of this work is to report the effectiveness, in the feline species, of a modified aglepristone administration protocol. Five intact queens were referred to veterinary care centers for pyometra. Aglepristone (15 mg/kg) was administered at D0, D2, D5, and D8, as described by Contri and collaborators for dogs. An antibiotic treatment (marbofloxacin, 3 mg/kg) was associated, and uterine conditions were checked with regular ultrasonographic exams. The uterus returned to its normal condition 10 days after starting the treatment and no adverse effects were reported. After treatment, three queens had an uneventful pregnancy. Even if the treated group was restricted and homogeneous, the proposed modified protocol proved to be useful and promising for the medical treatment of pyometra in cats; further studies are planned to verify its effectiveness in the long-term prevention of recurrence
Itinerari dusiani nel teatro di Elena Bucci. Intorno a "Non sentire il male"
No full textThe interview with Elena Bucci retraces dusian itineraries and the creative phases that traced the scenic writing of "Non sentire il male" (2000), performance dedicated to Eleonora Duse, whose Bucci is the author, actress and director. The indissoluble connection of the professional, artistic and private life of the Great Italian Actress is processed here through a personal weaving of documents and historical facts, which Bucci passes through the filter of an explicit autobiographical transfer: from her artistic training to the foundation of the company Le belle bandiere through the Teatro di Leo, moments in which the figure of Duse is always invoked like a “godmother”. During the interview, the salient passages of the conception and development of the performance are also reconstructed, not only in relation to the dramaturgy but also with respect to the staging and its different versions (site-specific, for black box, like a
live concert). Some contextual notes support the interview
Itinerari dusiani nel teatro di Elena Bucci. Intorno a 'Non sentire il male'
No full textThe interview with Elena Bucci retraces dusian itineraries and the creative phases that traced the scenic writing of "Non sentire il male" (2000), performance dedicated to Eleonora Duse, whose Bucci is the author, actress and director. The indissoluble connection of the professional, artistic and private life of the Great Italian Actress is processed here through a personal weaving of documents and historical facts, which Bucci passes through the filter of an explicit autobiographical transfer: from her artistic training to the foundation of the company Le belle bandiere through the Teatro di Leo, moments in which the figure of Duse is always invoked like a “godmother”. During the interview, the salient passages of the conception and development of the performance are also reconstructed, not only in relation to the dramaturgy but also with respect to the staging and its different versions (site-specific, for black box, like a
live concert). Some contextual notes support the interview
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