39 research outputs found
Concurrent Liquid Chromatographic Separation and Photodiode Array Detection of Retinol, Tocopherols, All-Trans-α-Carotene, All-Trans-β- Carotene and the Mono-Cis Isomers of β-Carotene in Extracts of Human Plasma
No full textIn this report we describe the development of a method for the concurrent reversed-phase high-performance liquid chromatographic separation and photodiode array detection of human plasma retinol, tocopherols and carotenes. For a single sample injection, retinol, retinyl acetate, α- tocopherol, γ-tocopherol, α-tocopheryl acetate, all-trans-α-carotene and all-trans-β-carotene, as well as the mono-cis geometrical isomers of β- carotene were separated and detected. Analytical separations were performed at a subambient temperature (0°C) over a Suplex pKb-100 reversed-phase analytical column with an isocratic mobile phase of methanol-methyl tert.- butyl ether-water (80:20:5, v/v/v) at a flow-rate of 0.8 ml/min for 60 min. Standards and samples were reconstituted in ethanol, and typically, 50 μl was injected for analysis. By HPLC, compounds of interest were clearly resolved and detectable at the picomole level
Investigation of the relationship among selected personality traits in beginning counselors
No full textThe Influence of Cysteine and Methionine Supplements on Polyamine Biosynthesis in the Rat
No full textPolyamine Biosynthesis in Sulfate Deficient and Sulfate Supplemented Rats
No full textAdult male albino Sprague Dawley rats were fed diets containing 15 % of casein with a constant 0.62 % of supplemental methionine, and three levels of inorganic sulfate, 0.0002 %, 0.02 %, and 0.42 %. The polyamines (putrescine, spermidine, and spermine) and the controlling enzymes for their biosynthesis, ornithine decarboxylase (ODC) and S-adenosyl-methionine decarboxylase (SAMD) were evaluated in liver tissue homogenates following a 17 day dietary period. There was no increase in the activity of ODC or the tissue concentration of putrescine in the liver tissue of rats fed the diet low in sulfate. There was an increase in SAMD activity and the concentration of spermidine and spermine. The activity of both ODC and SAMD and the tissue concentration of putrescine, spermidine, and spermine increased in the liver tissue from rats fed the diet high in inorganic sulfate. These data coupled with previous observations suggest that the metabolic effects of diets low in inorganic sulfate are probably not mediated through the regulatory activity of the polyamines. However, these data allow the suggestion that the metabolic alterations observed in rats fed diets high in inorganic sulfate may be due to the regulatory action of the polyamines
Fair Use in the Information Age
No full textThe application of the fair use doctrine to services which provide and associate information about a work with the work itself has proved to be an enigma. Even since Judge Leval’s seminal 1990 article, circuit courts have applied precedent to a new paradigm of available information inconsistently. In particular, the Second Circuit’s ruling in Fox News v. TVEyes represents a subtle step backward from that circuit’s progressive rulings in Authors Guild v. Google and Authors Guild v. HathiTrust. With little recent Supreme Court jurisprudence on fair use, and no rulings on critical uses of content made available by third party services such as TVEyes and Google Books, the TVEyes decision provides a perfect opportunity for the Supreme Court to refine the fair use framework in this context. This article argues that the Supreme Court ought to accept TVEyes’ petition for certiorari in order adopt what the author terms the “broad view” of fair use that the Second Circuit used in both Google Books and HathiTrust. The broad view of fair use appropriately balances the intellectual property rights provided under copyright law with incentivizing creative expression by both extending the market-harm rule in Campbell v. Acuff-Rose to information about a work and finding transformative use where the work or how it is accessed is transformed
Co-Administration of Paraquat and Putrescine to Rats via Miniosmotic Pump: Effects on Lung Glutathione Antioxidant System and PQ Content
No full textPhenylethanolamine-N-Methyl Transferase May Control Methionine Demethylation
No full textRats fed diets which contained 15% of casein and 0.620% of methionine with 0.0002, 0.02 and 0.42% of dietary inorganic sulfate had a dietary sulfate-related change in methionine metabolism. Rats fed the diets low in sulfate (0.0002%) had a 35% increase in methionine metabolism compared to rats fed the diets high in sulfate (0.42%). In contrast, rats fed the diet low in sulfate (0.0002%) had the lowest level of tissue S-adenosyl-methionine and the highest activity of phenylethanolamine-N-methyltransferase activity. Those animals fed the diet normal with respect to sulfate (0.02%) had intermediate levels of S-adenosylmethionine and phenylethanolamine-N-methyl transferase activity. Rats fed the diet high in sulfate (0.42%) had the highest level of tissue S-adenosyl-methionine and the lowest phenylethanol-amine-N-methyl transferase activity. Due to the inverse relationship between S-adenosylmethionine and phenylethanolamine-N-methyl transferase activity, it appears that the catecholamines may function as a methyl sink for the increase in the metabolism of methionine required to provide sulfate for rats fed diets low in sulfate
Delayed Hypersensitivity on a Surgical Service
No full textTwo hundred patients assessed for nutritional deficiencies were analyzed for reaction to skin testing with common antigens and the incidence of sepsis and death. Only 39% of all skin tests were positive, though 50% of the patients had at least one positive test. Associated diagnoses revealed a high incidence of malnutrition, cancer, radiation therapy, and chemotherapy. Analysis using Chi-square and Gamma shows good statistical correlation between skin tests and sepsis and death. Those with negative tests (anergic) using PPD, Candida, and mumps had a threefold higher mortality. Major sepsis also increased in the skin negative group (+80%), but here the incidence varied directly with the number of positive skin tests. Mortality, unlike sepsis, was influenced only by the presence of delayed hypersensitivity and was not related to the number of positive reactions. The basic rate of infection or mortality was not influenced by major surgery
