1,231 research outputs found
Selective impairments in self-ordered working memory in a patient with a unilateral striatal lesion
No full textA patient is described with a striatocapsular infarct resulting in damage to the left caudate-putamen, sparing the left ventral striatum and globus pallidus, who exhibits relatively selective deficits in self-ordered working memory performance. These deficits were shown repeatedly over several months, with several different types of test and material. The impairments were shown in the absence of obvious perceptual, visuospatial constructional, motor or language deficits. Recognition memory and cued recall performance was intact, although free recall was poor on some occasions. Aspects of executive performance were also intact, including set-shifting performance, but there were significant deficits in planning. The results are discussed in terms of the operation of parallel, functionally segregated cortico-striatal ‘loops’. © 1995, Taylor & Francis Group, LLC. All rights reserved
Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer.
No full textThe infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique beta-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall proteins (CWPs) composed of Leu-rich repeats (CWP(LRR)) and a C-terminal conserved Cys-rich region (CWP(CRR)). Our goals were to dissect the structure of the cyst wall and determine how it is disrupted during excystation. The intact Giardia cyst wall is thin (approximately 400 nm), easily fractured by sonication, and impermeable to small molecules. Curled fibrils of the GalNAc homopolymer are restricted to a narrow plane and are coated with linear arrays of oval-shaped protein complex. In contrast, cyst walls of Giardia treated with hot alkali to deproteinate fibrils of the GalNAc homopolymer are thick (approximately 1.2 microm), resistant to sonication, and permeable. The deproteinated GalNAc homopolymer, which forms a loose lattice of curled fibrils, is bound by native CWP1 and CWP2, as well as by maltose-binding protein (MBP)-fusions containing the full-length CWP1 or CWP1(LRR). In contrast, neither MBP alone nor MBP fused to CWP1(CRR) bind to the GalNAc homopolymer. Recombinant CWP1 binds to the GalNAc homopolymer within secretory vesicles of Giardia encysting in vitro. Fibrils of the GalNAc homopolymer are exposed during excystation or by treatment of heat-killed cysts with chymotrypsin, while deproteinated fibrils of the GalNAc homopolymer are degraded by extracts of Giardia cysts but not trophozoites. These results show the Leu-rich repeat domain of CWP1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. During excystation, host and Giardia proteases appear to degrade bound CWPs, exposing fibrils of the GalNAc homopolymer that are digested by a stage-specific glycohydrolase
House Unpassed Legislation 1859, rejected, SC1/series 230, Petition of F.M. Robbins [Frances M. Robbins]
No full textPetition subject: Against the rendition of fugitive slaves Original: http://nrs.harvard.edu/urn-3:FHCL:11029992 Date of creation: (unknown) Petition location: Milton Legislator, committee, or address that the petition was sent to: Mrs. Robbins, care of James M. Robbins, Esq., Mattapan, Mass.; John S. Eldridge, Canton; committee on federal relations Selected signatures:F.M. Robbins [Frances M. Robbins]Mary VoseRhoda Ferry Actions taken on dates: 1859-01-14,1859-01-15 Legislative action: Received in the House on January 14, 1859 and referred to the committee on federal relations and sent for concurrence and received in the Senate on January 15, 1859 and concurred Total signatures: 32 Legislative action summary: Received, referred, sent, received, concurred Legal voter signatures (males not identified as non-legal): 12 Female signatures: 14 Unidentified signatures: 6 Female only signatures: No Identifications of signatories: citizens, [females], ["others"] Prayer format was printed vs. manuscript: Printed Signatory column format: not column separated Additional non-petition or unrelated documents available at archive: no additional documents Location of the petition at the Massachusetts Archives of the Commonwealth: House Unpassed 1859, rejected Acknowledgements: Supported by the National Endowment for the Humanities (PW-5105612), Massachusetts Archives of the Commonwealth, Radcliffe Institute for Advanced Study at Harvard University, Center for American Political Studies at Harvard University, Institutional Development Initiative at Harvard University, and Harvard University Library.</p
Development Of A Preliminary Lifing Analysis Tool For The F135-PW-100 Engine
No full textIn the near future the Royal Netherlands Air Force will replace their fleet of F-16’s with the F-35. In the past the NLR has aided the Air Force with life cycle and deterioration analysis work on the F100-PW-220 engine, which powers the F-16. Understanding the physical system of the engine allows for on-condition maintenance. The same is preferred for the F135-PW-100 engine powering the F-35. Therefore, a preliminary lifing analysis tool has been developed for the F135-PW-100 engine rotor blades, based on open source literature. Aerospace Engineerin
House Unpassed Legislation 1859, rejected, SC1/series 230, Petition of F.M. Robbins [Frances M. Robbins]
No full textPetition subject: Against the rendition of fugitive slaves Original: http://nrs.harvard.edu/urn-3:FHCL:11029992 Date of creation: (unknown) Petition location: Milton Legislator, committee, or address that the petition was sent to: Mrs. Robbins, care of James M. Robbins, Esq., Mattapan, Mass.; John S. Eldridge, Canton; committee on federal relations Selected signatures:F.M. Robbins [Frances M. Robbins]Mary VoseRhoda Ferry Actions taken on dates: 1859-01-14,1859-01-15 Legislative action: Received in the House on January 14, 1859 and referred to the committee on federal relations and sent for concurrence and received in the Senate on January 15, 1859 and concurred Total signatures: 32 Legislative action summary: Received, referred, sent, received, concurred Legal voter signatures (males not identified as non-legal): 12 Female signatures: 14 Unidentified signatures: 6 Female only signatures: No Identifications of signatories: citizens, [females], ["others"] Prayer format was printed vs. manuscript: Printed Signatory column format: not column separated Additional non-petition or unrelated documents available at archive: no additional documents Location of the petition at the Massachusetts Archives of the Commonwealth: House Unpassed 1859, rejected Acknowledgements: Supported by the National Endowment for the Humanities (PW-5105612), Massachusetts Archives of the Commonwealth, Radcliffe Institute for Advanced Study at Harvard University, Center for American Political Studies at Harvard University, Institutional Development Initiative at Harvard University, and Harvard University Library.</p
Evidence for a Structural Role for Acid-Fast Lipids in Oocyst Walls of Cryptosporidium, Toxoplasma, and Eimeria
No full textCoccidia are protozoan parasites that cause significant human disease and are of major agricultural importance. Cryptosporidium spp. cause diarrhea in humans and animals, while Toxoplasma causes disseminated infections in fetuses and untreated AIDS patients. Eimeria is a major pathogen of commercial chickens. Oocysts, which are the infectious form of Cryptosporidium and Eimeria and one of two infectious forms of Toxoplasma (the other is tissue cysts in undercooked meat), have a multilayered wall. Recently we showed that the inner layer of the oocyst walls of Toxoplasma and Eimeria is a porous scaffold of fibers of β-1,3-glucan, which are also present in fungal walls but are absent from Cryptosporidium oocyst walls. Here we present evidence for a structural role for lipids in the oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria. Briefly, oocyst walls of each organism label with acid-fast stains that bind to lipids in the walls of mycobacteria. Polyketide synthases similar to those that make mycobacterial wall lipids are abundant in oocysts of Toxoplasma and Eimeria and are predicted in Cryptosporidium. The outer layer of oocyst wall of Eimeria and the entire oocyst wall of Cryptosporidium are dissolved by organic solvents. Oocyst wall lipids are complex mixtures of triglycerides, some of which contain polyhydroxy fatty acyl chains like those present in plant cutin or elongated fatty acyl chains like mycolic acids. We propose a two-layered model of the oocyst wall (glucan and acid-fast lipids) that resembles the two-layered walls of mycobacteria (peptidoglycan and acid-fast lipids) and plants (cellulose and cutin).
IMPORTANCE Oocysts, which are essential for the fecal-oral spread of coccidia, have a wall that is thought responsible for their survival in the environment and for their transit through the stomach and small intestine. While oocyst walls of Toxoplasma and Eimeria are strengthened by a porous scaffold of fibrils of β-1,3-glucan and by proteins cross-linked by dityrosines, both are absent from walls of Cryptosporidium. We show here that all oocyst walls are acid fast, have a rigid bilayer, dissolve in organic solvents, and contain a complex set of triglycerides rich in polyhydroxy and long fatty acyl chains that might be synthesized by an abundant polyketide synthase. These results suggest the possibility that coccidia build a waxy coat of acid-fast lipids in the oocyst wall that makes them resistant to environmental stress.National Institutes of Health (U.S.) (Grant GM31318
0.85 PW laser operation at 3.3 Hz and high-contrast ultrahigh-intensity λ = 400 nm second-harmonic beamline
No full textWe demonstrate the generation of 0.85 PW, 30 fs laser pulses at a repetition rate of 3.3 Hz with a record average power of 85 W from a Ti:sapphire laser. The system is pumped by high-energy Nd:glass slab amplifiers frequency doubled in LiB3O5 (LBO). Ultrahigh-contrast λ = 400 nm femtosecond pulses were generated in KH2PO4 (KDP) with>40% efficiency. An intensity of 6.5 × 1021 W∕cm2 was obtained by frequency doubling 80% of the available Ti:sapphire energy and focusing the doubled light with an f∕2 parabola. This laser will enable highly relativistic plasma experiments to be conducted at high repetition rate
Shifting attention in viewer- and object-based reference frames after unilateral brain injury
No full textThe aims of the present study were to investigate the respective roles that object- and viewer-based reference frames play in reorienting visual attention, and to assess their influence after unilateral brain injury. To do so, we studied 16 right hemisphere injured (RHI) and 13 left hemisphere injured (LHI) patients. We used a cueing design that manipulates the location of cues and targets relative to a display comprised of two rectangles (i.e., objects). Unlike previous studies with patients, we presented all cues at midline rather than in the left or right visual fields. Thus, in the critical conditions in which targets were presented laterally, reorienting of attention was always from a midline cue. Performance was measured for lateralized target detection as a function of viewer-based (contra- and ipsilesional sides) and object-based (requiring reorienting within or between objects) reference frames. As expected, contralesional detection was slower than ipsilesional detection for the patients. More importantly, objects influenced target detection differently in the contralesional and ipsilesional fields. Contralesionally, reorienting to a target within the cued object took longer than reorienting to a target in the same location but in the uncued object. This finding is consistent with object-based neglect. Ipsilesionally, the means were in the opposite direction. Furthermore, no significant difference was found in object-based influences between the patient groups (RHI vs. LHI). These findings are discussed in the context of reference frames used in reorienting attention for target detection
Supplementary Data: Spectral Control via Multi-Species Effects in PW-Class Laser-Ion Acceleration
No full textSupplementary materials for our paper "Spectral Control via Multi-Species Effects in PW-Class Laser-Ion Acceleration".
Additional high-resolution, raw HDF5 files using the openPMD standard (DOI:10.5281/zenodo.1167843) increase simulation output data to 4.7 TByte and are available from the corresponding author upon reasonable request.This project received funding within the MEPHISTO project (BMBF-Förderkennzeichen 01IH16006C)
Hillard, Mary Robbins, [1877] [June] [12]
No full textLetter Addressee changed to Kate Hillard by PW on 7/31/2008;paper copy; includes transcription from P. Walker. Redated from [05/19?] [1877].Brandeis Universit
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