628 research outputs found
ISOELECTRIC-FOCUSING IN NON-AMPHOTERIC BUFFERS - CATASTROPHE AND NON-CATASTROPHE THEORIES
No full textFour potential modes of isoelectric focusing in non-amphoteric buffers are evaluated:(a)"stack" or "train" of free bases or acids "arrested by a deprotonation or protonation mechanism", respectively (Chrambach); (b) "chemically bonded" (immobilized) pH gradients (Righetti et al.); (c) "physically bonded" or "quasi-immobilized" pH gradients (Bier et al.); (d) steady-state rheoelectrolysis (Rilbe). The first is based on a "catastrophe" theory, i.e., it confines the buffers in a pH region where they can create a pH gradient by an isotachophoretic mechanism, but where they do not have sufficient buffering capacity to stabilize it; no true isoelectric focusing can ever be achieved with this system. The last three are based on sound and well defined theories; however, at present, only system (b) (immobilized pH gradients) has proved to be a simple and reliable technique, easily transplantable in any laboratory. Bier et al.'s and Rilbe's approaches require complex and elaborate experimental set-ups and strict adherence of laboratory practice to a set of physical laws governing the system. In practice, owing to the divergence of experimental approaches from idealized physical equilibria, the last two approaches appear still to be far away from daily laboratory work
When public health communication intertwines with social media logic. A Focus on the Early Phase of COVID-19 Pandemic in Italy
No full textDuring recent years, public health organizations have faced the challenges of digitization, adopting new communication practices. The COVID-19 pandemic has impacted on this process, accelerating the digitization of healthcare communication. In this context, utilizing a theoretical framework that intertwines health communication and social media studies, this article investigates how local health authorities located in Lombardy (North of Italy) managed COVID-19 pandemic on their Facebook pages during the first phase of the emergency. The findings highlight the growing number of COVID-19 posts in the pages and a significant response from social media users in terms of interactions and engagement, reiterating the strategic role of these organizations in healthcare communication. Furthermore, they reveal a gradual adaptation of health communication to the dynamics of social media, especially in terms of popularity, programmability, and connectivity. The emergence of a platform-based approach to healthcare communication is also discussed
Righetti_et_al_Supplementary_materials - Outcomes Related to Antiplatelet Therapy in a High-Risk ST-Segment Elevation Myocardial Infarction Population: A Retrospective Real-World Analysis of an Italian ECMO Center
No full textRighetti_et_al_Supplementary_materials for Outcomes Related to Antiplatelet Therapy in a High-Risk ST-Segment Elevation Myocardial Infarction Population: A Retrospective Real-World Analysis of an Italian ECMO Center by Stefano Righetti, Elisabetta Montemerlo, Federica Soffici, Davide Sala, Alessandro Bozzano, Andrea Mauro, Elena Maggioni, Leonello Avalli, Paola Camisasca, Virgilio Colombo, Felice Achilli, Maddalena Lettino and Ivan Calchera in Journal of Cardiovascular Pharmacology and Therapeutics</p
Enzyme-mediated preparation of the single enantiomers of the olfactory active components of the woody odorant Timberol
No full textSoggetti affetti da disforia di genere, vittime ed autori di violenze: uno studio casistico
No full textè noto come sui soggetti che affrontano percorsi per la rettifica del sesso esistano molti pregiudizi che si allargano anche all’aspetto dei reati commessi e subiti da soggetti con tali caratteristiche personologiche. Finalità della presente ricerca casistica, è offrire un contributo di conoscenza a tale particolare aspetto clinico e criminologico relativo a questa particolare popolazione di soggett
Development of a flow process for an easy and fast access to 2-pyrone derivatives
No full text2-Pyrones are compounds widely present in nature and they represent interesting building blocks both in medicinal and synthetic chemistry. Due to their peculiar pharmacological activity and structure, they have attracted much attention during the last decades and several protocols for their synthesis have been developed. In this work we propose the synthesis of bio-sourced 2-pyrones, exploiting continuous-flow conditions for an easy, sustainable and fast access to these important molecules
Correction to: Size‐Dependent Enforcement, Tax Evasion and Dimensional Trap
No full textThe article “Size‐Dependent Enforcement, Tax Evasion and Dimensional Trap”, written by Raffaella Coppier, Elisabetta Michetti and Luisa Scaccia, was originally published electronically on the publisher’s internet portal on 05 July 2023 without open access. With the author(s)’ decision to opt for Open Choice the copyright of the article changed on 24 February 2024 to © The Author(s) 2024 and the article is forthwith distributed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made
Immobilized pH gradients
No full textIn this short review, we give an account of the steps through which the protocols for operation with IPGs were set up in the early '80s. One of the main achievements by our group was the development of a computer program, pH GRADIENT, for the calculation of pH, buffering power and ionic strength of a mixture of monoprotic buffers titrated within any pH span and for the linearization of the desired pH gradient. Using this program, in 1984 we could devise formulations for IPGs covering up to six pH units, which was the subject of a publication in Electrophoresis (volume 5, pages 88-97). This was the starting point for the use of IPGs for the resolution of protein samples of any composition and for their application as first dimension of 2-DE separations. Currently IPGs are in common use in proteomics investigations, not only along classical protocols but also for sample prefractionation and in shotgun approaches. Much less frequently are they used for 1-DE analytical applications, a field for which in recent years much attention has instead more often focused on capillary electrophoresis/IEF procedures
Blogs: life writing on the Net
No full textGenre analysis of personal blogs in relation to diaries and journals
Size and charge distribution of macromolecules in living systems
No full textWe report the statistical distributions of size and charge in protein molecules in living systems. The data have been plotted from more than 500 entries. 57% of the native proteins have molecular weights between 25,000 and 150,000 daltons. In oligomeric proteins, 71% of the subunits have molecular weights in the range 10,000-60,000. As for the subunit stoichiometry in oligomeric proteins, 50% of all the possible structures are dimeric, 30% tetrameric and 8% hexameric. More than one third of the proteins (38%) have pl values within 1.5 pH unit (pH4.5-6.0). 70% of the total proteins have pI values below pH 7 and 30% above it. On the basis of these data, guidelines are given for a purification strategy when using SDS-electrophoresis and isoelectric focusing
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