1,104 research outputs found
Phosphorylation-dependent dimerization and subcellular localization of islet-brain 1/c-Jun N-terminal kinase-interacting protein 1
No full textIslet-brain 1 [IB1; also termed c-Jun N-terminal kinase (JNK)-interacting protein 1 (JIP-1] is involved in the apoptotic signaling cascade of JNK and functions as a scaffold protein. It organizes several MAP kinases and the microtubule-transport motor protein kinesin and relates to other signal-transducing molecules such as the amyloid precursor protein. Here we have identified IB1/JIP-1 using different antibodies that reacted with either a monomeric or a dimeric form of IB1/JIP-1. By immunoelectron microscopy, differences in the subcellular localization were observed. The monomeric form was found in the cytoplasmic compartment and is associated with the cytoskeleton and with membranes, whereas the dimeric form was found in addition in nuclei. After treatment of mouse brain homogenates with alkaline phosphatase, the dimeric form disappeared and the monomeric form decreased its molecular weight, suggesting that an IB1/JIP-1 dimehzation is phosphorylation dependent and that IB1 exists in several phospho- forms. N-methyl-D-aspartate receptor activation induced a dephosphorylation of IB1/JIP-1 in primary cultures of cortical neurons and reduced homodimehzation. In conclusion, these data suggest that IB1/JIP-1 monomers and dimers may differ in compartmental localization and thus function as a scaffold protein of the JNK signaling cascade in the cytoplasm or as a transcription factor in nuclei
Iron Age and Roman copper alloys from northern Britain.
No full textSIGLEAvailable from British Library Document Supply Centre- DSC:DX185540 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
A Lagrangian sub-grid model for the dispersion of clouds of tracers
No full textTurbulence models are expected to satisfy the conflicting requirements of accuracy and computational efficiency. Here we discuss a new model that was recently developed in order to accurately and efficiently describe the dynamic of a clouds of tracers particles in Large Eddy Simulations of homogeneous and isotropic turbulent flows. The models incorporates the multi-scale nature of time and space turbulent velocity correlations that are essential in order to correctly reproduce the relative dispersion of multi-particle clouds. The model can be seen as an off-grid solver for the Eulerian velocity field at the positions of a given number of Lagrangian tracers that self-consistently move with it. Extensions to non homogeneous and isotropic turbulence as well as to the dynamics of particles will be discussed
I.M. Panagiotopoulos as an autobiographer: the adventure of the hero and the personal myth of the author
No full textThe present doctoral dissertation refers to the complex and prominent image of I.M. Panagiotopoulos as a human being, which emerges through his exuberant and diverse work. This dissertation explains man with work and work with man and highlights a special aspect of I.M. Panagiotopoulos that of the pedagogue, through his pedagogical texts, his presence in the classroom and, in general, his pedagogical action. It refers to his childhood, repulsed mostly to the unconscious and to the deeper layers of his psyche. Also, the object of this study is the novel writing of I.M. Panagiotopoulos which istreated as a consequence of the denaturation of his obstructed erotic energy. Next, the two dominant impulses, Love and Death that meet in art and in life, are explored, with the impulses of Death predominant, a fact that leads to their narcissistic character, as well as to their conflicting nature. The anthropogeographical and metaphysical elements that constitute the concept and ontology of Greece are then highlighted. Then, the pedagogical work of I.M. Panagiotopoulos, his positions,contradictions and his proposals for Education is examined. I.M. Panagiotopoulos fought for a school that will offer Liberal Education, within a democratic regime that defines the equality of opportunity in learning. In addition, our national language is presented during its historical course and its expressive and iconographic boldness is highlighted. Finally, this thesis codifies the evolutionary stages of a single hero and creates a typology of his inner adventure, which is the adventure of the hero's desire. It also traces the author's personal myth on a general level, the correspondences and the dialectical relationship between the author and his work, in such a one-way way, that the work defines and composes the author and not vice versa.Η παρούσα διδακτορική διατριβή αναφέρεται στη σύνθετη κα εξέχουσα εικόνα του Ι.Μ. Παναγιωτόπουλου ως ανθρώπου, η οποία ξεπροβάλλει μέσα από το πληθωρικό και πολύμορφο έργο του. Εξηγεί τον άνθρωπο με το έργο και το έργο με τον άνθρωπο. Αναδεικνύει μια ξεχωριστή πλευρά του Ι.Μ. Παναγιωτόπουλου αυτή του Παιδαγωγού, μέσα από τα παιδαγωγικά του κείμενα, από την παρουσία του στην τάξη και γενικότερα από την παιδαγωγική του δράση. Αναφέρεται στην παιδική του ηλικία απωθημένη ως επί το πλείστον στο ασυνείδητο και στα βαθύτερα στρώματα του ψυχισμού του. Επίσης, αντικείμενο της μελέτης είναι η μυθιστορηματική γραφή του Ι.Μ. Παναγιωτόπουλου που αντιμετωπίζεται ως απόρροια μετουσίωσης της παρεμποδισμένης ερωτικής του ενέργειας. Ακολούθως, διερευνώνται οι δύο κυρίαρχες ενορμήσεις, του Έρωτα και του Θανάτου, που ανταμώνουν στην τέχνη και στη ζωή, με κυρίαρχες μάλιστα τις ενορμήσεις του Θανάτου, γεγονός που οδηγεί στον ναρκισσικό χαρακτήρα τους, καθώς και στη συγκρουσιακή τους φύση. Στη συνέχεια, αναδεικνύονται τα ανθρωπογεωγραφικά και μεταφυσικά στοιχεία που συνιστούν την έννοια και την οντολογία της Ελλάδας. Κατόπιν, εξετάζεται το Παιδαγωγικό του έργο και παρουσιάζονται οι θέσεις, οι αντιθέσεις και οι προτάσεις του Ι.Μ. Παναγιωτόπουλου για την Παιδεία. Ο Ι.Μ. Παναγιωτόπουλος αγωνίστηκε για ένα σχολείο, το οποίο θα προσφέρει Αγωγή φιλελεύθερη, μέσα σ’ ένα δημοκρατικό καθεστώς που ορίζει την ισότητα των ευκαιριών στη μάθηση. Επιπλέον, παρουσιάζεται η εθνική μας γλώσσα κατά την ιστορική της διαδρομή και αναδεικνύεται η εκφραστική και εικονοπλαστική της τόλμη. Τέλος, η παρούσα διατριβή κωδικοποιεί τα εξελικτικά στάδια ενός ενιαίου ήρωα και δημιουργεί μια τυπολογία της εσωτερικής του περιπέτειας, που είναι η περιπέτεια της επιθυμίας του ήρωα. Επίσης, ανιχνεύονται ο προσωπικός μύθος του συγγραφέα σε ένα γενικό επίπεδο, οι αντιστοιχίες και η διαλεκτική σχέση ανάμεσα στον συγγραφέα και στο έργο του, με τέτοιο μονοδρομικό τρόπο, ώστε το έργο να καθορίζει και να συνθέτει τον συγγραφέα και όχι αντίθετα
Thiol-reactive protein labeling of brain proteins with infrared DY-680 and DY-782 maleimides
No full textMethods and software for significant indicators determination of the natural language texts author profile
No full textMethods for the formation and optimization of author profiles are presented. The author profile is an image – a vector in a multidimensional space, which components are author’s texts measurements by a number of methods based on 4-grams, stemming, recurrence analysis and formal stochastic grammar. The author’s profile is a model of his language, including vocabulary, sentence syntax features. A comparative analysis of each of the methods effectiveness is carried out. By means of the genetic algorithm, a reduced profile of the author is formed. Insignificant indicators are excluded, which allows to reduce their number by 20%. The reduced author’s profile contains attributes that are significant for this author and is an effective attribution of a particular author.Prombles in programming 2023; 3: 22-2
Information about the Golden Horde in the Saltuk-name of the Ottoman author Ebul-Khayri Rumi
No full textThe purpose of the study is to analyze information on the history of the Golden Horde from the multi-volume work of Ebul-Khayri Rumi “Saltuk-name”, an Ottoman author of the 15th century.
Research materials. Ottoman historical works contain quite original information about the Golden Horde and the Tatar khanates. Their information allows us to reveal previously unknown pages of the history of the Golden Horde period. One of such important and early works is the three-volume work “Saltuk-name” by Ebul-Khayri Rumi, an Ottoman author of the 15th century, describing the legendary history of Sary-Saltuk, who was engaged in the spread of Islam, including in the lands of the Golden Horde, primarily in Crimea. In Russian-language historiography, this author is little known and his information has not been used for scientific research. The relevance of the article is explained by the need to introduce into scientific circulation the information of the “Saltuk-name” about the Golden Horde.
Results and scientific novelty. The study is the first attempt at a systematic analysis of Ebul-Khayri Rumi’s Saltuk-name in Russian. The novelty of the study is the introduction of specific information from the Ottoman historical work and its interpretation into scientific circulation. This study provides an important impetus for the introduction of information from the works of Ottoman authors who wrote about the Golden Horde into active scientific circulation
Measurements of fluid flow in weld pools
No full textUnderstanding the fluid flow in weld pools contributes significantly toward controlling the heat distribution in the base material and the mass distribution of molten base and additive materials. Currently, most investigations focus primarily on numerical models, due to the experimental difficulties associated with the challenging environment in and around the weld pool. Numerical simulations based on computational fluid dynamics are currently addressing the dynamic behaviour of weld pools, such as melting, solidification, temperature and velocity fields; these results provide information about conditions inside the weld pool that is impossible to gain experimentally. However, there is still a shortage of experimental validation to support these models. In this thesis, experimental methods are applied to study the fluid flow of the weld pool in both arc and laser generated weld pools. The general conclusions from the experimental study provides some suggestions for understanding the fluid flow in the weld pool, such as flow asymmetry, rotational flow and interface instabilities. For example, the interface instabilities can affect the energy and mass distribution. Describing these instabilities numerically requires three dimensional flow models. Moreover, the current experimental results question the validity of works where the surfactant concentration is assumed to be constant in the weld pool without consideration of dynamic diffusion and convection. This work has focused on experimental measurement of the fluid dynamics of weld pools, and current results provide a number of validation cases for further numerical simulations.Materials Science & EngineeringMechanical, Maritime and Materials Engineerin
Microstructural Evolution during High-Frequency Post Weld Impact Treatments for High-Strength Steels
No full textMaterial Science and EngineeringMechanical, Maritime and Materials Engineerin
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