161 research outputs found
Arctic algal genomes supporting data
No full textThis database presents supporting data and archived paper drafts for a pan-genomic analysis of convergent evolution in distantly related Arctic algae (Dorrell et al, ibid)
pr2database/pr2database: PR2 version 5.0.0
No full textContributors
Daniel Vaulot - General curation, Diatoms
Javier del Campo, Fabien Burki, Mahwash Jamy , Laure Guillou - Taxonomy - 9 levels update
Luciana Santoferrara, Maximilian Ganser - Ciliates
Luciana Santoferrara - Mixoplankton database
Andrea de Oliveira da Rocha Franco - Diatoms (4 genera)
Kenneth Mertens, Haifeng Gu, Se Hyeon Jang - Dinoflagellates
Pavel Škaloud - Chrysophyceae
Manon Dünn - Percolomonads
Megan Gross - Microsporidia - Metchnikovellida
Alexei Seliuk - Chytrids and Fungi_X
Miguel Sandin - Radiolaria
Sebastian Metz - Perkinsea
Anna Maria Fiore-Donno- Myxogastria
Richard Dorrell - 16S plastid
See detailed changes and download flat files from https://github.com/pr2database/pr2database/releases/tag/v5.0.0Download flat files from https://github.com/pr2database/pr2database/releases/tag/v5.0.
Dance 35
No full textProgram: Clap Hands ; Choreographer: Rosanne Barton-DeVries Ondegierra ; Choreographer: Linda Graham & Dancers Tribal Suite (1989) ; Choreographer: Steven Iannacone On Common Ground ; Choreographer: Dorrell Martin What the Fluff?! ; Choreographer: Amanda Smith ephemeral episodes ; Choreographer: Anne-Rene Petrarca 212 Degrees ; Choreographer: Richard River
Arctic algal genomes supporting data
No full textThis database presents supporting data and archived paper drafts for a pan-genomic analysis of convergent evolution in distantly related Arctic algae (Dorrell et al, ibid)
Instantaneous representation and the pig itself
No full textInstantaneous representation offers the promise of showing reality itself, but as all representation removes context, it still relies on what are traditionally considered to be elements of writing, rather than existing in opposition to writing. Narrative is essential in any form of representation, as the proliferation of talk radio, 'reality television', docu-dramas, etc. shows. Virtual reality can also be seen as a type, or a continuation, of the writing process. Furthermore, editing, whether in television or photography, makes the delivery of 'reality' an idiosyncratic process rather than an impartial reporting, even in the case of instantaneous representation (i.e. live television).PT: J; CR: AUSTIN JL, 1962, SENSE SENSIBILIA BENJAMIN W, 1936, WORK ART AGE MECH RE BORGMANN A, 1999, HOLDING REALITY NYIRI JC, 1992, TRADITION INDIVIDUAL POSTER M, 1999, CYBERSPACE TEXTUALIT, P42 RING M, 1972, PERSONALIST, V53, P357 RYAN ML, 1999, SUBSTANCE, V28, P110 SELTZER M, 1998, SERIAL KILLERS SEY J, 1999, CYBERPSYCHOLOGY, P25 TIROHL B, 2000, NEW MEDIA SOC, V2, P335 VARELA EJ, 1997, EMBODIED MIND VICK RG, 1972, PERSONALIST, V53, P348; NR: 12; TC: 0; J9: NEW MEDIA SOC; PG: 10; GA: 631TNSource type: Electronic(1
Démêler l'architecture unique du transporteur de chloroplastes des algues diatomées Phaeodactylum tricornutum : aperçu des analyses informatiques et expérimentales
No full textLes diatomées sont un contributeur clé à la photosynthèse marine. Les diatomées sont apparentées de loin aux plantes et possèdent des chloroplastes (plastes) d'origine rouge secondaire distincts des plastes primaires des plantes, avec un métabolisme associé mal compris. Ici, nous utilisons des données transcriptomiques, phylogénomiques et environnementales pour construire un atlas in silico des transporteurs de plastes prédits identifiés par des recherches à l'échelle du génome de l'espèce de diatomée modèle Phaeodactylum tricornutum. Notre analyse a conduit à la découverte d'un nouveau transporteur de la superfamille MFS, désormais appelé "Trans3". Le gène codant pour ce transporteur se trouve dans les espèces contenant des plastes rouges secondaires et montre une forte corrélation positive d'expression avec les gènes codés dans le génome mitochondrial de Phaeodactylum. À l'aide de la GFP auto-assemblée, nous avons localisé Trans3 au niveau de la membrane périplastidiale plastidiale (PPM). Nous avons généré trois mutants hétérozygotes Trans3 CRISPR-Cas9 probables qui montrent une réduction de 20 % de la croissance cellulaire et une teneur en photosystème I inférieure à celle des lignées témoins, ce qui implique qu'il joue un rôle important dans la croissance des cellules de diatomées et l'efficacité photosynthétique. Fait intéressant, nos lignées mutantes ont montré moins de sensibilité au stress d'appauvrissement en Fe que les témoins de vecteurs vides, ce que nous déduisons des données transcriptomiques pour se rapporter à une régulation à la hausse de l'expression de la synthèse des pigments et des voies liées au complexe de collecte de la lumière qui peuvent atténuer la photosensibilité knock-out à Fe épuisement. Enfin, compte tenu des données structurelles, nous suggérons que Trans3 fonctionne comme un transporteur de molybdate similaire à la protéine de transport de molybdate 2 (MOT2) trouvée dans Chlamydom onas, qui peut participer au transport du molybdate à travers les membranes plastidiales. Les mesures préliminaires indiquent que les mutants knock-out avaient des taux d'absorption de molybdate plus élevés que les témoins de vecteurs vides, bien que cela puisse être affecté par la disponibilité de NO3 et de Fe dans les milieux de croissance. Dans l'ensemble, ces données fournissent de nouvelles informations sur la manière dont les transporteurs de plastides contribuent au succès retentissant des diatomées dans l'océan moderne.Diatoms are a key contributor to marine photosynthesis. Diatoms are distantly related to plants, and possess chloroplasts (plastid) of secondary red origin distinct from the primary plastids of plants, with a poorly understood associated metabolism. Here, we use transcriptomic, phylogenomic and environmental data to construct an in silico atlas of predicted plastid transporters identified by genome-wide searches of the model diatom species Phaeodactylum tricornutum. Our analysis led to the discovery of a novel MFS superfamily transporter, henceforth termed “Trans3”. The gene encoding this transporter is found in species containing secondary red plastids, and shows a strong positive correlation of expression with genes encoded in the Phaeodactylum mitochondrial genome. Using self-assembling GFP, we located Trans3 at the plastid peri-plastidial membrane (PPM). We generated three probable heterozygotic Trans3 CRISPR-Cas9 mutants which show a 20% reduction in cell growth, and lower photosystem I content than control lines implying that it plays important roles for diatom cell growth and photosynthetic efficiency. Interestingly, our mutant lines showed less sensitivity to Fe depletion stress than empty vector controls, which we infer from transcriptomic data to relate to an upregulation of expression of pigment synthesis and light-harvesting complex-related pathways that may mitigate knockout photo-sensitivity to Fe depletion. Finally, considering structural data, we suggest Trans3 functions as a molybdate transporter similar to the molybdate transporter 2 (MOT2) protein found in Chlamydomonas, which may participate in molybdate transport through the plastid membranes. Preliminary measurements indicate that knockout mutants had higher molybdate uptake rates than empty vector controls, although this may be affected by the availability of NO3 and Fe in growth media. Overall, these data provide new insights into how plastid transporters contribute to the striking success of diatoms in the modern ocean
Description of the Development of a Title III Program and its Impact on a School District
No full textEducational Administratio
Sheet Metal Workers' International Association charter
No full textSheet Metal Workers' International Association charter, local union #146, Springfield, Missouri. Legible names on the document include Charles G. Gibson, Gary L. Comer, Randy L. Smith, J. Michael Dailey, David V. Maples, William W. Newlon Jr., Donald L. Atwood Sr., John F. Brown, John D. Nash, John C. White, Steven L. Bench, Steven C. Brake, Mark D. Becraft, Dorrell K. Jenkins, Bryan K. Hedrick, Elvin Joe Nichols, Gary D. Brigance, Kenneth E. Stokes, Scott R. Stewart, Charles G. Vodicka, Roger L. Fields, Paul W. Fairchild Jr., Richard Berg, Bradley J. Loveland, Michael L. Berg, Lawrence J. Cassidy and Arthur Moore
Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes
No full textAlthough the major food-borne pathogen Campylobacter jejuni has been isolated from diverse animal, human and environmental sources, our knowledge of genomic diversity in C. jejuni is based exclusively on human or human food-chain-associated isolates. Studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources. Using comparative genomic hybridization on a collection of 80 isolates representing diverse sources and clonal complexes, we identified a separate clade comprising a group of water/wildlife isolates of C. jejuni with multilocus sequence types uncharacteristic of human food-chain-associated isolates. By genome sequencing one representative of this diverse group (C. jejuni 1336), and a representative of the bank-vole niche specialist ST-3704 (C. jejuni 414), we identified deletions of genomic regions normally carried by human food-chain-associated C. jejuni. Several of the deleted regions included genes implicated in chicken colonization or in virulence. Novel genomic insertions contributing to the accessory genomes of strains 1336 and 414 were identified. Comparative analysis using PCR assays indicated that novel regions were common but not ubiquitous among the water/wildlife group of isolates, indicating further genomic diversity among this group, whereas all ST-3704 isolates carried the same novel accessory regions. While strain 1336 was able to colonize chicks, strain 414 was not, suggesting that regions specifically absent from the genome of strain 414 may play an important role in this common route of Campylobacter infection of humans. We suggest that the genomic divergence observed constitutes evidence of adaptation leading to niche specialization
Impacts fonctionnels de l'évolution horizontale du génome chez les algues eucaryotes
No full textEukaryotic micro-organisms, and particularly algae, play essential roles in the Earth ecosystem, as primary producers, in nutrient cycling, and at the basis of aquatic food webs. Algae have a complex and polyphyletic evolutionary history, which has involved the repeated endosymbiotic acquisition of chloroplasts, and horizontal gene transfers with both algal and bacterial partners into the nuclear genomes. The most abundant algae in the contemporary ocean, such as dinoflagellates, haptophytes and diatoms, possess chloroplasts acquired through the secondary or higher endosymbioses of red algae. These chloroplasts further are supported by complex, nucleus-encoded proteomes, which can be considered to be mosaics, defined by the entire evolutionary history of the host. My group studies how the horizontal evolution of algae with secondary red chloroplasts informs their current functional biology and ecology. This involves three complementary projects: computational reconstruction of the nucleus-encoded proteomes of algal chloroplasts, with a particular focus on dinoflagellates; experimental and environmental characterisation of novel proteins supporting the diatom chloroplast; and phylogenetic evaluation of the broader horizontal gene transfers underpinning algal evolution, with a particular focus on haptophytes and distantly-related species native to the Arctic Ocean. Finally, I consider how these combined stories and approaches may intertwine to inform our understanding of algal sensitivity and resilience to anthropogenic climate change.Les micro-organismes eucaryotes, et en particulier les algues, jouent un rôle essentiel dans l'écosystème mondiale, en tant que producteurs primaires, dans le cycle des nutriments et à la base des réseaux alimentaires aquatiques. Les algues ont une histoire évolutive complexe et polyphylétique, due à l'acquisition endosymbiotique répétée de chloroplastes et des transferts horizontaux de gènes avec des partenaires algaux et bactériens dans les génomes nucléaires. Les algues les plus abondantes dans l'océan contemporain, telles que les dinoflagellés, les haptophytes et les diatomées, possèdent des chloroplastes originaires des endosymbioses secondaire ou supérieure d'algues rouges. Ces chloroplastes sont soutenus par des protéomes complexes codés par le noyau, qui peuvent être considérés comme des mosaïques définies à travers de l'histoire évolutive de l'hôte.Mon équipe étudie comment l'évolution horizontale des algues informe leur biologie fonctionnelle et leur écologie actuelle. Cela implique trois projets complémentaires : la reconstruction computationnelle des protéomes des chloroplastes d'algues encodés dans le noyau, avec une emphase sur les dinoflagellés ; la caractérisation expérimentale et environnementale de nouvelles protéines soutenant le chloroplaste des diatomées ; et l'évaluation phylogénétique des transferts horizontaux de gènes plus larges qui sous-tendent l'évolution des algues, avec un accent particulier sur les haptophytes et les espèces autochtones de l'océan Arctique. Enfin, je considère comment ces histoires et approches combinées peuvent s'entrelacer pour éclairer notre compréhension de la sensibilité et de la résilience des algues au changement climatique anthropique
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