406 research outputs found
Ein Stolperstein für Lothar Keiner
No full textAkte im Osnabrücker Landesarchiv dokumentiert das Schicksal eines Gelsenkircheners NS-Opfers Gefangenenkarteikarte des Lothar Keiner aus der Strafanstalt Lingen mit Angaben zu seiner Person, seiner Verurteilung und der Dauer seiner Inhaftierung.(NLA OS, Rep 947 Lin II Nr. 15404) In Gelsenkirchen-Horst wurde am 23. Mai 2018 ein Stolperstein des Kölner Künstlers Gunter Demnig für Lothar Keiner verlegt. Er erinnert an die Verfolgung Keiners im Dritten Reich (vgl. http://www.stolpersteine-gelsen..
Methodological aspects of the SAVE data set
No full textThis paper describes the general design of the SAVE survey: the design of the questionnaire, inter-viewer and interviewee motivation, and the sampling designs of the various subsamples collected in 2001 and 2003. It discusses the representativeness of the data, explains the construction of weights, and provides probit regressions to analyse potential selectivity problems. The paper finishes by discussing implications for the use of the SAVE data in various estimation procedures.
SORTCERY—A High–Throughput Method to Affinity Rank Peptide Ligands
No full textUncovering the relationships between peptide and protein sequences and binding properties is critical for successfully predicting, re-designing and inhibiting protein–protein interactions. Systematically collected data that link protein sequence to binding are valuable for elucidating determinants of protein interaction but are rare in the literature because such data are experimentally difficult to generate. Here we describe SORTCERY, a high-throughput method that we have used to rank hundreds of yeast-displayed peptides according to their affinities for a target interaction partner. The procedure involves fluorescence-activated cell sorting of a library, deep sequencing of sorted pools and downstream computational analysis. We have developed theoretical models and statistical tools that assist in planning these stages. We demonstrate SORTCERY's utility by ranking 1026 BH3 (Bcl-2 homology 3) peptides with respect to their affinities for the anti-apoptotic protein Bcl-x[subscript L]. Our results are in striking agreement with measured affinities for 19 individual peptides with dissociation constants ranging from 0.1 to 60 nM. High-resolution ranking can be used to improve our understanding of sequence–function relationships and to support the development of computational models for predicting and designing novel interactions.National Institutes of Health (U.S.) (Award GM096466)German Academic Scholarship Foundation (Grant RE 3111/1-1
Generating High-Accuracy Peptide-Binding Data in High Throughput with Yeast Surface Display and SORTCERY
No full textLibrary methods are widely used to study protein-protein interactions, and high-throughput screening or selection followed by sequencing can identify a large number of peptide ligands for a protein target. In this chapter, we describe a procedure called "SORTCERY" that can rank the affinities of library members for a target with high accuracy. SORTCERY follows a three-step protocol. First, fluorescence-activated cell sorting (FACS) is used to sort a library of yeast-displayed peptide ligands according to their affinities for a target. Second, all sorted pools are deep sequenced. Third, the resulting data are analyzed to create a ranking. We demonstrate an application of SORTCERY to the problem of ranking peptide ligands for the anti-apoptotic regulator Bcl-xL.National Institute of General Medical Sciences (U.S.) (Award GM096466
Against the mainstream, nazi privatization in 1930s Germany.
No full textThe Great Depression spurred State ownership in Western capitalist countries. Germany was no exception, the last governments of the Weimar Republic took over firms in diverse sectors. Later, the Nazi regime transferred public ownership and public services to the private sector. In doing so, they went against the mainstream trends in the Western capitalist countries, none of which systematically reprivatized firms during the 1930s. Privatization in Nazi Germany was also unique in transferring to private hands the delivery of public services previously provided by government. The firms and the services transferred to private ownership belonged to diverse sectors. Privatization was part of an intentional policy with multiple objectives and was not ideologically driven. As in many recent privatizations, particularly within the European Union, strong financial restrictions were a central motivation. In addition, privatization was used as a political tool to enhance support for the government and for the Nazi Party.Privatization, Public Enterprise, Nazi Economy, Germany.
Orthopedics in the Third Reich - Lothar Kreuz and the University of Berlin 1937-1945
No full textDie vorliegende Promotion versteht sich als Beitrag zur Erforschung der
Orthopädie im Nationalsozialismus. Anhand eines der exponiertesten Orthopäden
im Dritten Reich soll die Entwicklung der Fachdisziplin in den Jahren von 1933
bis 1945 analysiert werden. Hierzu wurde sämtliche Literatur von und über
Lothar Kreuz ausgewertet sowie eine ausführliche Literaturrecherche der
orthopädischen Fachbeiträge der Jahre 1933-55 durchgeführt, als auch Material
in den einschlägigen Archiven gesichtet. Besondere Schwerpunkte wurden hierbei
bei der Frage der Bewertung von schweren körperlichen Missbildungen zur
Anwendung auf das Gesetz zur Verhütung erbkranken Nachwuchses als auch bei der
Etablierung einer professionellen Ohnhänderversorgung im Rahmen der
Rehabilitation von schweren Kriegsverletzungen gelegt. Lothar Kreuz gelang
während der nationalsozialistischen Diktatur eine steile Karriere bis an die
Spitze der renommierten Friedrich-Wilhelms-Universität in Berlin. Er trat
bereits 1933 sowohl der NSDAP als auch der SS bei und stieg bis zum Kriegsende
zum SS-Standartenführer auf. Laut der Aussage einer Krankenschwester in einem
SS-Ehrengerichtsverfahren berichtete Kreuz im Operationssaal des Oskar-Helene-
Heims, zwei Tage nach der Ermordung der SA-Führung am 30. Juni 1934, dass er
als Exekutivarzt bei den Verhören und der anschließenden Erschießung zugegen
war. Trotz dieser Tatsachen gelang es ihm im Nachkriegsdeutschland, nachdem er
in seinem Entnazifizierungsverfahren als „Entlasteter“ eingestuft worden war,
an der Eberhard-Karls-Universität in Tübingen erneut Karriere zu machen und
1958 als Krönung seiner Laufbahn das große Verdienstkreuz des Verdienstordens
der BRD verliehen zu bekommen.This doctoral thesis is a contribution to the research of orthopedics during
National Socialism. The development of the discipline of orthopedics from 1933
to 1945 is being analyzed on the basis of one of the most prominent orthopedic
surgeons in the Third Reich. In order to achieve this available references
about Lothar Kreuz have been investigated. Furthermore, a detailed analysis of
orthopedic papers of the years 1933-55 was conducted and accessible data in
corresponding archives got screened. Essential emphasis has been put on the
assessment of severe congenital disorders in the context of the Law for the
Prevention of Genetically Diseased Offspring (which was a statute in Nazi
Germany enacted in 1933), as well as of the medical care for war veterans who
suffered the loss of both hands. Lothar Kreuz climbed the professional ladder
during the Nazi dictatorship - up to the top of the prestigious Friedrich
Wilhelms University in Berlin. As early as 1933, he became a party member of
the NSDAP and got recruited by the SS where he carried the rank of SS-
Standartenführer at the end of the war. According to testimony by a nurse in a
SS-disciplinary court, Lothar Kreuz performed as a doctor in the interrogation
an subsequent execution of the SA-command on June 30th 1934. Despite these
facts, he succeeded in post-war Germany. After he had been discharged by a
denazification trial, Kreuz managed to stage a comeback at the Eberhard Karls
University in Tübingen and - as the culimination of his curriculum vitae - in
1958 he got awarded the Order of Merit of the Federal Republic of Germany
Predictive Bcl-2 Family Binding Models Rooted in Experiment or Structure
No full textProteins of the Bcl-2 family either enhance or suppress programmed cell death and are centrally involved in cancer development and resistance to chemotherapy. BH3 (Bcl-2 homology 3)-only Bcl-2 proteins promote cell death by docking an α-helix into a hydrophobic groove on the surface of one or more of five pro-survival Bcl-2 receptor proteins. There is high structural homology within the pro-death and pro-survival families, yet a high degree of interaction specificity is nevertheless encoded, posing an interesting and important molecular recognition problem. Understanding protein features that dictate Bcl-2 interaction specificity is critical for designing peptide-based cancer therapeutics and diagnostics. In this study, we present peptide SPOT arrays and deep sequencing data from yeast display screening experiments that significantly expand the BH3 sequence space that has been experimentally tested for interaction with five human anti-apoptotic receptors. These data provide rich information about the determinants of Bcl-2 family specificity. To interpret and use the information, we constructed two simple data-based models that can predict affinity and specificity when evaluated on independent data sets within a limited sequence space. We also constructed a novel structure-based statistical potential, called STATIUM, which is remarkably good at predicting Bcl-2 affinity and specificity, especially considering it is not trained on experimental data. We compare the performance of our three models to each other and to alternative structure-based methods and discuss how such tools can guide prediction and design of new Bcl-2 family complexes.National Institutes of Health (U.S.) (Award GM067681)National Science Foundation (U.S.) (Grant 0821391
König Lothar III. an Bistum Bamberg - 6.6.1134
No full textArchivsignatur: Ks.selekt Nr.459{'name': 'DFG', 'uri': 'dfg.png'
Kaiser Lothar I. an Granfelden, Kloster - 25.8.849
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Kaiser Lothar III. an Bursfelde, Kloster - 26.5.1134
No full textzu 09:beschädigt; Archivsignatur: Kloster Bursfelde Nr.2a{'name': 'DFG', 'uri': 'dfg.png'
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