199,385 research outputs found
L’héritage des empereurs romains… : M. Romero Recio (coord.), El legado de los emperadores hispanos, Sevilla, 2018
No full textPintado Javier Andreu. L’héritage des empereurs romains… : M. Romero Recio (coord.), El legado de los emperadores hispanos, Sevilla, 2018. In: Dialogues d'histoire ancienne, vol. 45, n°2, 2019. pp. 445-449
Bernardo Recio, un jesuita euskaldunberri del siglo XVIII
No full textEn este trabajo abordaremos el estudio de la fi gura del jesuita castellano Bernardo Recio. Destinado en el año 1740 al colegio que la Compañía tenía en Oñati, Recio decidió aprender el euskera, contando para ello con la asistencia de la gramática vasca que pocos años atrás había publicado el también jesuita Manuel Larramendi. Consiguió su propósito en el breve plazo de tres meses y fue tal su dominio del euskera que llegó a escribir poesías en dicha lengua e incluso, según refiere el propio Larramendi, “enmendaba a los que la hablan con menos propriedad”.
Palabras clave: Bernardo Recio. Manuel Larramendi. Aprendizaje del euskera. Siglo XVIII . Jesuitas. Oñati.Lan honetan, Bernardo Recio jesuita espainiarraren bizitza aztertuko dugu. 1740an, Jesusen Lagundiaren Oñatiko ikastetxera bidali zuten, eta Reciok euskara ikastea erabaki zuen. Horretarako, Manuel Larramendi jesuitak zenbait urte lehenago argitaratutako euskal gramatika erabili zuen. Hiru hilabetean hizkuntza ikastea lortu zuen, eta hain maila handia lortu zuen, non poesiak ere idazten baitzituen euskaraz. Horrez gainera, Larramendik esan zuen bezala “zuzentasunez hitz egiten ez zutenei akatsak zuzentzen zizkien”.
Gako hitzak: Bernardo Recio. Manuel Larramendi. Euskara ikastea. XVIII . mendea. Jesuitak. Oñati
Rotation velocities of hot horizontal branch stars in the globular clusters NGC 1904, NGC 2808, NGC 6093, and NGC 7078: The database
No full textWe present radial and rotation (v sin i) velocity measurements from UVES+VLT high resolution spectra of 61 stars in the blue horizontal branches (HB) of the Galactic globular clusters NGC 1904 (M 79), NGC 2808, NGC 6093 (M 80) and NGC 7078 (M 15).
The data reduction and the velocity determination, based on cross-correlation techniques, are discussed in detail. Most of this database has been used by Recio-Blanco et al. (\cite{Recio02}) in their analysis of the rotation velocity properties of blue HB stars. Here we present additional data for NGC 2808. We confirm the results of the previous paper, in particular, a possible link between the HB jump and the abrupt change in the rotational velocity distribution around Teff ̃ 11 500 K.
Based on observations with the ESO Very Large Telescope + UVES, at the Paranal Observatory, Chile.
Tables 2-5 are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/417/59
Empirical studies of the construction of discourse/ edited by Óscar Loureda, Inés Recio Fernández, Laura Nadal and Adriana Cruz.
No full textIncludes bibliographical references and index.Introduction / Óscar Loureda, Inés Recio Fernández, Adriana Cruz and Laura Nadal -- Part I. Corpus-based studies: 1. Challenges in the contrastive study of discourse markers: the case of then / Karin Aijmer -- 2. Local vs. global scope of discourse markers: Corpus-based evidence from syntax and co-occurring pauses / Ludivine Crible -- 3. Prosodic versatility, hierarchical rank and pragmatic function in conversational markers / Antonio Hidalgo Navarro and Diana Martínez Hernández -- 4. A preliminary typology of interactional figures based on a tool for visualizing conversational structure / Guadalupe Espinosa-Guerri and Amparo García-Ramón -- 5. Causal relations between discourse and grammar: Because in spoken French and Dutch / Liesbeth Degand -- 6. A corpus-based comparative study of concessive connectives in English, German and Spanish: The distribution of although, obwohl and aunque in the Europarl corpus / Volker Gast -- Part II. Experiment-based studies: 7. Processing patterns of focusing in Spanish / Adriana Cruz and Óscar Loureda -- 8. Expectation changes over time: How long it takes to process focus imposed by German sogar / Johannes Gerwien and Martha Rudka -- 9. Processing implicit and explicit causality in Spanish / Laura Nadal and Inés Recio Fernández -- Part III. Combined approaches: 10. Subjectivity and Causality in discourse and cognition: Evidence from corpus analyses, acquisition and processing / Ted J. M. Sanders and Jacqueline Evers-Vermeul -- 11. Causal connectives revisited: A corpus and experimental investigation of result discourse markers in English / Marta Andersson.1 online resource
La chute de l’empire romain en questions : M. Romero Recio (coord.), La caída del Imperio Romano. Cuestiones historiográficas, (Potsdamer Altertumswissenschaftliche Beiträge, 53), 2016
No full textAlvar Nuño Antón. La chute de l’empire romain en questions : M. Romero Recio (coord.), La caída del Imperio Romano. Cuestiones historiográficas, (Potsdamer Altertumswissenschaftliche Beiträge, 53), 2016. In: Dialogues d'histoire ancienne, vol. 43, n°2, 2017. pp. 320-325
Role of Methylation in Modulating MAPK Signaling. Implications in Melanoma. Papel de la metilación como modulador de la señalización en MAPK. Implicaciones en melanoma.
No full text1. General Overview
MTAP is a key enzyme in the methionine salvage pathway and controls the levels of methylthioadenosine (MTA). Lost of MTAP, which is frequent in melanoma, results in higher intra and extracellular MTA levels. As MTA is an inhibitor of methylation reactions within the cell, we decided to further investigate the role of methylation in the progression of cancer and how the main signaling pathway involved in melanoma (RAS-RAF-MEK-ERK pathway) can be affected in the presence or absence of methylation inhibitors.
2. Results
• Exogenous addition of MTA (and therefore blocking of methylation reactions) to melanoma cells increased total activity of the RAS-ERK pathway, highlighting a new role of methylation into controlling the amplitude of the signal transduction.
• We found out that PRMT5, a protein arginine methyl transferase enzyme, binds to CRAF, a key component of the RAS-ERK pathway. In vitro and in vivo experiments showed that PRMT5 methylates CRAF and thus modifies the pathway’s output. We identified CRAF arginine 563 as the target of PRMT5 methyl transferase activity. Furthermore, in vitro kinase assays and siRNA technology experiments showed that methylation at arginine 563 can modify the kinase activity of CRAF protein, therefore modulating the total activity of the RAS-ERK pathway.
• The previous results provided the first known evidence of methylation as a key modulator of the activity in MAPKs pathways, so we looked for a biological significance of this new regulatory mechanism. We used the well-established PC12 cell line system (EGF growth factor triggers proliferation whereas NGF promotes differentiation): as we had shown that PRMT5-catalyzed methylation can modify the kinase activity of the RAS-ERK pathway, we wondered whether this regulation could be part of a general mechanism controlling cell behavior. In order to test this, we tried to mimic the differentiating effect of NGF using EGF to activate the pathway in the presence of MTA or after PRMT5 siRNA. Interestingly, when triggering the cells with EGF after PRMT5 depletion or MTA treatment, they differentiated in a manner indistinguishable from that induced by NGF. Moreover, we observed that cells transfected with CRAFR563K mutant underwent differentiation, instead proliferation, when treating the cells with EGF. Together these data demonstrate a central role of methylation in the growth factors-elicited biological responses.
• RAS-ERK pathway plays a key role into controlling development and maintenance of melanoma. There is a clear correlation between the activity of the pathway and melanoma growth and proliferation, so we wondered how methylation reactions could affect melanoma progression. In order to address this question we performed in vitro assays in a variety of melanoma cell lines and we found out that MTA inhibited cell proliferation and viability. Next, we injected these cells in a melanoma xenograft mouse model to induce tumoral growth: mice treated with MTA showed a significant decrease (47%) in the tumor volume with no apparent toxic effects, indicating that MTA is effective blocking melanoma in vivo tumor growth. Besides, we have been able to proof that MTA inhibitory effect was due to citostatic MTA capability rather than a pro-apoptotic effect.
3. Discussion
In our reports we demonstrate that methylation is an important mechanism modulating signal transduction through the RAS-ERK pathway in response to specific growth factors. Our data implicate a specific PRMT5 methylation motif (GRG) that is conserved in all RAF proteins and we demonstrate that methylation appears to be a critical posttranslational modification controlling the RAF input to the downstream activity.
We have shown that methylation is controlling ERK signal amplitude in response to specific growth factors in cells from different species. We also have identified PRMT5 as the protein performing the methylation reaction. Our results provide clues about the possible fine-tuning mechanism by which methylation regulates the total signal, indicating that PRMT5 methylates CRAF at arginine 563.
Our research shows that protein arginine methylation is a universal mechanism that modulates signal transduction in response to specific growth factors and we demonstrate that PRMT5 is the methyltransferase involved in the process regulating the total CRAF kinase activity within the pathway. The mechanism we describe provides the means by which cells can modulate the time profile of ERK activation for which a particular biological response is evoked. More importantly, because cancer cells tend to heavily rely on oncogenic signalling through the RAS-RAF-MEK-ERK pathway, this additional level of signal transduction regulation identifies novel candidate targets for therapeutical intervention.
Finally, we have tested the potential of the methylation inhibitor MTA regarding to tumour progression: our experiments have demonstrated that MTA inhibits, in vitro, cell proliferation and viability in a dose dependent manner in a variety of mouse and human melanoma cell lines. Importantly, MTA was also effective inhibiting in vivo tumour growth in a mouse melanoma xenograft model. In summary, here we show the therapeutic potential of the natural occurring nucleoside MTA and we demonstrate that MTA can inhibit melanoma cell proliferation and in vivo tumour growth at non-toxic rates, supporting the use of MTA in antitumoral therapies and presenting protein methylation reactions as potential targets when developing new antitumoral strategies
La chute de l’empire romain en questions : M. Romero Recio (coord.), La caída del Imperio Romano. Cuestiones historiográficas, (Potsdamer Altertumswissenschaftliche Beiträge, 53), 2016
No full textAlvar Nuño Antón. La chute de l’empire romain en questions : M. Romero Recio (coord.), La caída del Imperio Romano. Cuestiones historiográficas, (Potsdamer Altertumswissenschaftliche Beiträge, 53), 2016. In: Dialogues d'histoire ancienne, vol. 43, n°2, 2017. pp. 320-325
La supercomputació per entendre la Terra
No full textLa supercomputació per entendre la Terra per José M. Baldasano Recio. Director del Departament de Ciències de la Terra Barcelona Supercomputing Cente.
Jornades Professors Secundària. Ramon Llull
La supercomputació per entendre la Terra
No full textLa supercomputació per entendre la Terra per José M. Baldasano Recio. Director del Departament de Ciències de la Terra Barcelona Supercomputing Cente.
Jornades Professors Secundària. Ramon Llull
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