87 research outputs found
Study of YAP/TAZ effectors of the Hippo pathway in gastric cancer
No full textLe cancer gastrique (CG) est la 5ème cause de mortalité par cancer dans le monde en 2020, avec un taux de survie à 5 ans inférieur à 25%. La voie de signalisation Hippo, impliquée dans le contrôle de la taille des organes et l’homéostasie tissulaire, joue un rôle clé dans la tumorigenèse du CG, en régulant les cofacteurs transcriptionnels YAP et TAZ. SCRIB, une protéine régulatrice de la polarité cellulaire, pourrait jouer un rôle dans la régulation de la voie Hippo. L’extinction de SCRIB par un siARN induit une diminution de la prolifération et de la migration des lignées de CG (AGS et KATO-III) ainsi qu’une diminution de l’expression des gènes canoniques de la voie Hippo (CTGF, CYR61, ANKRD1). Le rôle pro-prolifératif de SCRIB dans ces lignées pourrait être associé à sa mauvaise localisation cellulaire. L’impact de YAP et TAZ sur la prolifération des lignées de CG a été évalué. L’extinction de YAP/TAZ par des siARN diminue significativement la prolifération des lignées AGS et MKN-74. Une analyse transcriptomique (RNAseq) a révélé que l’extinction de YAP et TAZ perturbe l'expression des gènes impliqués dans la réponse intégrée au stress (ISR), notamment ceux contrôlés par le facteur de transcription ATF4. Ce travail met en lumière une interconnexion entre les facteurs YAP/TAZ et ATF4 et leur impact sur la prolifération et la survie des cellules cancéreuses gastriques. De plus, YAP/TAZ participe à la résistance des lignées de CG (AGS) à des traitements anticancéreux, tels que l'oxaliplatine, et le sorafenib. Enfin, un troisième volet de mon travail a porté sur la mise en place de modèles d’organoïdes et de tumoroïdes gastriques. En conclusion, cette étude met en lumière la complexité et l'hétérogénéité du cancer gastrique. SCRIB semble avoir un rôle pro-prolifératif dans certaines lignées de CG, en lien avec la dérégulation de la voie Hippo, tandis que les protéines YAP/TAZ apparaissent essentielles à la survie et à la sensibilité aux traitements chimiothérapeutiques de lignées de CGGastric cancer (GC) is the 5th leading cause of cancer death worldwide in 2020, with a 5-year survival rate of less than 25%. The Hippo signaling pathway, involved in organ size control and tissue homeostasis, plays a key role in GC tumorigenesis, by regulating transcriptional cofactors YAP and TAZ. SCRIB, a cell polarity regulatory protein, may play a role in regulating the Hippo pathway. Knockdown of SCRIB by siRNA induced a decrease proliferation and migration of CG lines (AGS and KATO-III), as well as a decrease in the expression of canonical Hippo pathway genes (CTGF, CYR61, ANKRD1). The pro-proliferative role of SCRIB in these cell lines might be associated with its cellular mislocalization. The impact of YAP and TAZ on the proliferation of CG lines was assessed. The siRNA- mediated silencing of YAP/TAZ significantly reduced the proliferation of AGS and MKN-74 cell lines. Transcriptomic analysis (RNAseq) revealed that YAP and TAZ silencing disrupts the expression of genes involved in the integrated stress response (ISR), notably those controlled by the ATF4 transcription factor. This work highlights an interconnection between the YAP/TAZ and ATF4 factors and their impact on GC cell proliferation and survival. Moreover, YAP/TAZ were involved in the sensitivity of CG lines (AGS) to anticancer drugs such as oxaliplatin and sorafenib. Finally, a third aspect of my work involved the establishment of gastric organoid and tumoroid models. In conclusion, this study highlights the complexity and heterogeneity of gastric cancer. SCRIB appears to have a pro-proliferative role in some GC lines, linked to deregulation of the Hippo pathway, while YAP/TAZ proteins appear essential for survival and sensitivity to chemotherapeutic treatments in GC lines
The effectiveness of nurses’ psychosocial interventions for sensory deprivation in intensive care patients: a systematic review and meta-analysis
No full textObjective: This systematic review and meta-analysis aimed to assess the effectiveness of nurses’ psychosocial interventions for addressing sensory deprivation in intensive care units (ICUs). Materials and methods: A comprehensive search of PubMed, Web of Science, EBSCOhost, Google Scholar, CİNAHL, Embase, Cochrane Library, and YÖK Thesis Center databases was conducted from August 2023 to May 2024, without any temporal restrictions. In addition, a physical search was made in the university library for grey literature. Results: The study revealed that nurses’ psychosocial interventions significantly improved patients’ level of consciousness (SMD = 1.042, %95 CI = 0.716 to 1.369; Z = 6.25; p <.05) and sleep quality in ICUs (SMD=1.21, 95% CI= 0.232 to 1.810; Z = 2.49; p <.05). The effectiveness of psychosocial interventions varied based on the type of intervention, patient age, ICU type, patient group, and intervention duration. Notably, auditory stimuli and aromatherapy demonstrated particularly high effect sizes, significantly enhancing patients’ levels of consciousness and sleep quality. Conclusion: In conclusion, psychosocial interventions aimed at reducing sensory deprivation in intensive care units exert beneficial effects on individuals, notably enhancing their level of consciousness and improving sleep quality. © The Author(s) 2024
Examination of the work of Mehmet Vecihi's Sevda-yı Masumane
No full textEdebi yazının farklı biçimlerde meydana gelmesi söylem alanlarının genişlemesine, faklı formlarla duygu ve düşüncelerin yaratımına imkân verir. Çok çeşitlilik arz eden edebi türler sanatkârların tüm canlı ve cansız varlıkları evrende konumlandırmasında yardımcı olur. Bir araç niteliği gören mektup türü ve tekniği ifade biçimlerine başka bir boyut kazandırır. Mektup hikâyenin Türk edebiyatında modern anlamda ilk örnekleri Tanzimat devri ile birlikte görülür. Mektup hikâye nesir türündeki metinlerde hem bütünlüklü bir yapıda hem de metnin içinde müstakil bir şekilde yer alarak günümüze kadar ulaşır. Mektup hikâye formunda kaleme aldığı Sevda-yı Masumane adlı eseriyle Servet-i Fünun dönemi yazarlarından olan Mehmet Vecihi yazdığı hikâye ve romanlarla devrinin tanınmış sanatkârlarındandır. Yazarın meydana getirdiği eserlerin niceliksel fazlalığına rağmen Latin harflerine aktarılmaması nedeniyle günümüzde bilinirliği azdır. Dolayısıyla Mehmet Vecihi'nin eserlerinden biri olan Sevda-yı Masumane mektup hikâyesini günümüz Türkçesine aktarmak, edebiyat dünyasındaki yerini belirlemek ve yazarın edebi yönünün izlerini sürmek önem arz etmektedir. Bu çalışma, Türk edebiyatı sahasında daha önce üzerinde hiç durulmamış olması bakımından tercih sebebi sayılan Sevda-yı Masumane adlı eserin gün yüzüne çıkarılmasını ve yapı-tema düzleminde irdelenerek bundan sonraki çalışmalara kaynak teşkil etmesini amaçlamaktadır. Anahtar Sözcükler: Mektup hikâye, Mehmet Vecihi, Sevda-yı Masumane, Yapı ve İzlekThe emergence of literary texts in different forms enables the expansion of the fields of discourse and the creation of feelings and thoughts with different forms. A wide variety of literary genres help artists to position all living and non-living beings in the universe. The letter type and technique, which is seen as a tool, adds another dimension to the forms of expression. The first modern examples of the letter-story in Turkish literature are seen in the Tanzimat period and they survive in prose type texts both in a holistic structure and independently in the text. Mehmet Vecihi, one of the writers of the Servet-i Fünun period with his work called Sevda-yı Masumane, which is written in the form of a letter-story, is one of the well-known artists of his era with his stories and novels. Despite the quantitative excess of the works produced by the author, they are not known today because not transferred to Latin letters. Therefore, it is important to transfer the story of Sevda-yı Masumane, which is one of the works of Mehmet Vecihi, into contemporary Turkish literature, to determine its place in the world of literature and to trace the literary aspect of the author. This study aims to unearth the work called Sevda-yı Masumane, which is considered as a reason for preference in the field of Turkish literature, since it has never been emphasized before, and to be a source for future studies by examining it on the basis of structure-theme. Key Word: Letter Story, Mehmet Vecihi, Sevda-yı Masumane, Structure and Them
The effect of nonpharmacological interventions applied by nurses to intensive care patients on the duration of delirium: a meta-analysis study
No full textObjective: It was aimed to determine the effect level of nonpharmacological methods applied by nurses to patients hospitalized in the intensive care unit on the duration of delirium. Materials and methods: For this study, relevant studies were accessed by searching in July–October 2022. After the necessary exclusions were made, 14 studies were included in the study. The total sample size of the studies was 1123. Results: According to the results, nonpharmacological interventions applied by nurses to intensive care patients were found to reduce the duration of delirium (SMD: − 0.625, 95% CI: − 1.1040–0.210; Z = − 2.950, p = 0.003, I 2 = 93.119%). The country of the study (SMD: − 0.047, p = 0.001) and the types of nonpharmacological interventions used (SMD: − 0.062, p = 0.000) influenced the effect size of modulators on the duration of delirium in ICU patients. Conclusion: Nonpharmacological interventions applied by nurses, who have significant responsibilities in the protection and promotion of health, were found to reduce the duration of delirium in intensive care patients. This study shows that nurses, one of the main components of the multidisciplinary team in intensive care, are successful when they apply nonpharmacological interventions well. © 2023, The Author(s), under exclusive licence to Royal Academy of Medicine in Ireland.PMID: 3762445
Disease burden and symptom management in type 2 diabetic patients: A phenomenological study
No full textAim: This study was conducted to address the experiences of diabetic patients from a psychosocial perspective, examine the burden of disease, and determine their practices for symptom management. Materials and Methods: In this study, a phenomenological research design, a qualitative research design, was used. The study included 20 diabetic patients. Individual and face-to-face interviews were performed, and Colaizzi's phenomenological analysis method was used to analyze the data. Results: As a result of the data analysis, four categories (challenges with care and treatment, coping with the disease, effects, and healthy life behaviors) and eleven main themes (knowledge, application, symptoms, support resources, spiritual coping methods, physical coping methods, physical effects, spiritual effects, social effects, physical practices, and psychological practices) emerged. Conclusion: It was concluded that the disease burden of diabetic patients is intense and that the participants of the study were not effective in coping with the disease through symptom management and found spirituality important in coping. © 2024 The Author(s). Public Health Nursing published by Wiley Periodicals LLC
The psychological dimension of menopause: a phenomenological study
No full textThis study aims to determine the perceptions of women in menopause living in Turkey regarding this period and associated problems, which has shown that some women are negatively affected by the menopause period and have difficulty coping with the effects of menopause. The study has a basic qualitative research design, and the sample was determined by using the criterion sampling method, one of the purposeful sampling methods. The effects and characteristics of menopause themes are included in the category of perception of menopause among women, and the themes of positive and negative perspectives are included in the category of perspective on menopause. It is reported that women experience feelings of fear, uncertainty, irritability, anxiety/worry, introversion, relaxation, and happiness during menopause. Our study has shown that some women are negatively affected by the menopause period and have difficulty coping with the effects of menopause. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature
Investigation of perceived parental attitude in children exposed to sexual abuse: A case-control study
No full textBackground: The enduring impact of children being subjected to sexual abuse is characterized by lasting traumatic effects. Aims: This study aims to assess the influence of perceived parental attitudes on the incidence of sexual abuse in children who have experienced such abuse, comparing them with a control group. Methods: The research took place at the Child Advocacy Center (CAC) of a hospital in northern Turkey and in the pediatric outpatient clinics of the same institution. The study evaluated 101 children who were exposed to sexual abuse and 100 children who were not. Data collection took place between November 2022 and August 2023. The study employed a ‘Descriptive Information Form’ and the ‘Parental Attitude Scale’ for both case and control groups. The t-test for independent samples, descriptive statistics, and logistic regression analysis were used for data analysis. Results: Analysis of scores from the Parental Attitude Scale revealed significantly lower mean scores in acceptance-involvement and strictness-supervision in the case group (p <.05). The mean scores for ‘acceptance-involvement’ (X¯ = 22.82 ± 6.26, X¯ = 25.71 ± 3.89) were highest in the case and control groups. Neglectful attitude (38.6%) was more prevalent in the case group, while the control group exhibited a higher frequency of democratic attitude (37%). In terms of predictors, acceptance-involvement (OR = 1.091, p =.005) and maternal employment (OR = 4.410, p <.001) were identified as the most significant predictors of exposed to sexual abuse. The study’s Cronbach’s alpha value for the scale was α =.743. Conclusion: The study’s findings on parental attitudes suggest a potential connection between children’s exposure to sexual abuse and inadequate parental acceptance, involvement, supervision, and control. © The Author(s) 2024
Disease Burden and Sexual Life Experiences of Individuals with Chronic Obstructive Pulmonary Disease: A Phenomenological Study
No full textThis study aimed to examine the disease burden encountered by individuals with COPD, with a comprehensive investigation of the effect of the disease on sexual life. In this study, a phenomenological research design, a qualitative research type, was employed. The study involved 36 individuals diagnosed with COPD. The data collection process involved conducting individual, face-to-face interviews. Colaizzi’s phenomenological analysis method was utilized for the data analysis. The analysis of the data yielded two categories (burdens of the disease and sexual life) and six primary themes (physical effects, mental effects, social effects, difficulties during sexual activity, the spouse’s perspective on the problems, and dealing with sexual problems). The outcomes of this study highlighted the significant burden of COPD on patients and its adverse impact on their sexual lives. Given the complex nature of COPD, a thorough and professional assessment of patients’ sexual well-being, coupled with timely identification of sexual dysfunctions and appropriate interventions, is of utmost importance. © 2024, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature
Experiences of Turkish mothers of children with autism: a phenomenological study
No full textThe study aimed to explore mothers’ experiences of children with autism using a phenomenological approach. Semi-structured, in-depth interviews were conducted with 18 parents with children attending a special education centre in a northern province of Turkey. Criterion sampling, a form of purposive sampling, was employed to select participants. Interviews continued until data saturation was achieved. All interviews were audio-recorded and transcribed verbatim. Thematic analysis was utilized to analyze the data, and the study adhered to the COREQ checklist for reporting qualitative research. As a result of analyses, three main themes (the process of diagnosis, the effect of the child’s illness on parents and their challenges over time, and considerations regarding the child’s educational journey) and eight sub-themes (feelings and thoughts, reflections on the causes, reactions encountered, emotional effects, social effects, physical effects, perception of social support, and expectations from the educator and the education process) emerged. The study concluded that mothers of children with autism are emotionally, physically, and socially impacted by their child’s condition, facing considerable challenges in coping. In light of these findings, it is recommended that policymakers in our country develop plans that address the difficulties expressed by participants, review and potentially enhance financial and social support for families, and consider increasing special education hours. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024
Earthquake diaries: Psychosocial difficulties and life experiences of nurses working in the disaster zone after the earthquake: A phenomenological study
No full textAim and Objectives: The aim of this study was to evaluate the psychosocial difficulties and life experiences of nurses working in the disaster area following the earthquake on February 6, Kahramanmaraş centered earthquake with a phenomenological approach. Background: After natural disasters such as earthquakes, nurses play a significant role in providing both physical and psychosocial support. Therefore, the experiences of nurses in this field are necessary both to support them and to assist disaster survivors. Materials and Methods: In this study, in which the phenomenological research method was used, semi-structured in-depth interviews with 18 nurses who provided health care services during the February 6 Kahramanmaraş earthquake were conducted between January and February 2024 via Whatsapp mobile application. Snowball sampling method was used to reach the sample group. Interviews continued until data saturation was achieved. All interviews were audio recorded and then transcribed. The data were analyzed using thematic analysis. The study was conducted and reported according to the COREQ checklist. Results: Data analysis revealed two themes (psychological and social difficulties caused by the earthquake, traumatic stress and coping experiences as a nurse) and five sub-themes (social difficulties, psychological difficulties, traumatic events, reactions and coping). Conclusion: As a result of the study, it was determined that nurses providing health care services were negatively affected psychosocially by the traumatic stress situations they experienced in the earthquake centered in Kahramanmaraş on February 6. It was determined that nurses had great difficulty in coping with the traumatic stress situations they experienced. It was concluded that the February 6 earthquake caused secondary traumatization in nurses. © 2024 The Author(s). Public Health Nursing published by Wiley Periodicals LLC
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