7,866 research outputs found
The Small Ubiquitin-related Mmodifier in the Stress Response and the Use of Mass Spectrometry/SUMmOn for Identification of Ubiquitin and Ubiquitin-like Protein Conjugation Sites
No full textUbiquitin (Ub) and the ubiquitin-like proteins (Ubls) are polypeptides that can be covalently conjugated to a variety of “target” molecules to modulate their turnover rate, localization and/or function. The full range of Ubl functions is only beginning to be understood. The Raught lab is using mass spectrometry and high throughput screening methods, along with standard cell biology and biochemistry approaches, to better understand Ubl function. Here, I describe the role of a Ubl called small ubiquitin-related modifier (SUMO) in the budding yeast alcohol stress response. We identified a regulatory mechanism of the SUMO system, involving modulation of the localization of a SUMO protease. Secondly, using mass spectrometry (MS), I assisted in identifying several yeast and mammalian Ubl “chain” linkages. Finally, I propose an integrated MS methodology designed to complement standard database software for the confident identification of Ub/Ubl conjugation sites.MAS
The Small Ubiquitin-related Mmodifier in the Stress Response and the Use of Mass Spectrometry/SUMmOn for Identification of Ubiquitin and Ubiquitin-like Protein Conjugation Sites
No full textUbiquitin (Ub) and the ubiquitin-like proteins (Ubls) are polypeptides that can be covalently conjugated to a variety of “target” molecules to modulate their turnover rate, localization and/or function. The full range of Ubl functions is only beginning to be understood. The Raught lab is using mass spectrometry and high throughput screening methods, along with standard cell biology and biochemistry approaches, to better understand Ubl function. Here, I describe the role of a Ubl called small ubiquitin-related modifier (SUMO) in the budding yeast alcohol stress response. We identified a regulatory mechanism of the SUMO system, involving modulation of the localization of a SUMO protease. Secondly, using mass spectrometry (MS), I assisted in identifying several yeast and mammalian Ubl “chain” linkages. Finally, I propose an integrated MS methodology designed to complement standard database software for the confident identification of Ub/Ubl conjugation sites.MAS
Author Interview with Brian D. Anderson
No full textBrian D. Anderson was our feature artist of the week, October 19th - 23rd, 2020.https://jagworks.southalabama.edu/vid_presentations/1010/thumbnail.jp
Competition policy. by Brian Ellis
No full texttag=1 data=Competition policy. by Brian Ellis
tag=2 data=Ellis, Brian
tag=3 data=Australian Rationalist,
tag=5 data=46
tag=6 data=Autumn/Winter 1998
tag=7 data=51-56.
tag=8 data=ECONOMIC CONDITIONS
tag=9 data=COMPETITION%CORPORATISATION%NATIONAL COMPETITION POLICY%PRIVATE SECTOR PUBLIC SECTOR EFFECTIVENESS%SERVICE DELIVERY%SOCIAL POLICY%INNOVATION
tag=10 data=Examines the Government's National Competition Policy in relation to encouraging R&D, and the corporisation of public services and utilites. The author is Emeritus Professor of Philosophy at La Trobe UNiversity and Vice-President of the Rationalist Society of Australia. Article Taken from What's New.
tag=13 data=CABExamines the Government's National Competition Policy in relation to encouraging R&D, and the corporisation of public services and utilites. The author is Emeritus Professor of Philosophy at La Trobe UNiversity and Vice-President of the Rationalist Society of Australia. Article Taken from What's New
Art Behind Gaming: Brian D. Anderson
No full textA discussion with author Brian D. Anderson about worldbuilding in fantasy. Part of the Art Behind Gaming Online Con.https://jagworks.southalabama.edu/vid_presentations/1046/thumbnail.jp
PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN
No full textCentrosome structure, function, and number are finely regulated at the cellular level to ensure normal mammalian development. Here, we characterize PPP1R35 as a novel bona fide centrosomal protein and demonstrate that it is critical for centriole elongation. Using quantitative super-resolution microscopy mapping and live-cell imaging we show that PPP1R35 is a resident centrosomal protein located in the proximal lumen above the cartwheel, a region of the centriole that has eluded detailed characterization. Loss of PPP1R35 function results in decreased centrosome number and shortened centrioles that lack centriolar distal and microtubule wall associated proteins required for centriole elongation. We further demonstrate that PPP1R35 acts downstream of, and forms a complex with, RTTN, a microcephaly protein required for distal centriole elongation. Altogether, our study identifies a novel step in the centriole elongation pathway centered on PPP1R35 and elucidates downstream partners of the microcephaly protein RTTN.</p
In Honour of Brian MacWhinney: A Personal Account
No full textWhile this volume and the writings have made it amply clear what significant contributions Professor Brian MacWhinney has made to the field at large, in this afterword, we begin with a senior member of our author team (Ping Li, PL) followed by a mid-career member (Helen Zhao, HZ) and an early career member (Zhe Gao, ZG), to provide our personal accounts of Brian not only as a leading scholar but also as a role model who touches and changes people’s lives
Interview with Brian Alleyne, Sociologist Studying KDE
No full textA few months ago, the British journal Sociology published an article titled "Challenging Code: A Sociological Reading of the KDE Free Software Project". Eager to find out what a 'sociological reading' of KDE entails, Dot editor Oriol Mirosa rushed to contact the article's author, sociologist Brian Alleyne, who graciously and patiently agreed to be the subject of an interview
Understanding Author Rights
No full textAuthor Rights is the term used to describe a researcher\u27s rights related to their published work. In this session, Brian Young will: 1) provide an overview of author rights, 2) explain language often used in the publication agreement, and 3) demonstrate a tool (Sherpa Romeo) that can be used to quickly understand what default rights you have (and lose) when you publish with a specific journal
Shady trading on the rights market. by Brian Pollard
No full texttag=1 data=Shady trading on the rights market. by Brian Pollard
tag=2 data=Pollard, Brian
tag=3 data=New Doctor,
tag=6 data=Winter 1995
tag=7 data=11-12.
tag=8 data=EUTHANASIA
tag=10 data=Because the spotlight of public attention has been strongly focused on doctors in this debate, the author believes that it is essential that every doctor makes a clear distinction between his or her private views on the practice of euthanasia and its legislation, because the implications in each case are simply not comparable.
tag=11 data=1995/1/5
tag=12 data=95/0224
tag=13 data=CABBecause the spotlight of public attention has been strongly focused on doctors in this debate, the author believes that it is essential that every doctor makes a clear distinction between his or her private views on the practice of euthanasia and its legislation, because the implications in each case are simply not comparable
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