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ADA newsletter
No full textThis archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Porous hydrogel scaffolds integrating Prussian Blue nanoparticles: A versatile strategy for electrochemical (bio)sensing
No full textElectrochemical detection of miRNA using commercial and hand‐made screen‐printed electrodes: liquid biopsy for cancer management as case of study
No full textThe growth of liquid biopsy, i. e., the possibility of obtaining health information by analysing circulating species (nucleic acids, cells, proteins, and vesicles) in peripheric biofluids, is pushing the field of sensors and biosensors beyond the limit to provide decentralised solutions for nonspecialists. In particular, among all the circulating species that can be adopted in managing cancer evolution, both for diagnostic and prognostic applications, microRNAs have been highly studied and detected. The development of electrochemical devices is particularly relevant for liquid biopsy purposes, and the screen-printed electrodes (SPEs) represent one of the building blocks for producing novel portable devices. In this work, we have taken miR-2115-3p as model target (it is related to lung cancer), and we have developed a biosensor by exploiting the use of a complementary DNA probe modified with methylene blue as redox mediator. In particular, the chosen sensing architecture was applied to serum measurements of the selected miRNA, obtaining a detection limit within the low nanomolar range; in addition, various platforms were interrogated, namely commercial and hand-made SPEs, with the aim of providing the reader with some insights about the optimal platform to be used by considering both the cost and the analytical performance.Screen-printed electrodes are able to offer potent tools for detecting miRNAs in liquid biopsy: commercial or handmade? imag
Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform
No full text: Thrombin overexpression in serum serves as a critical biomarker and is implicated in several diseases associated with significant morbidity and mortality. Existing techniques for thrombin detection are time-consuming and require sophisticated equipment and extensive sample preparation procedures, which further delay the detection and increase the cost of the procedure. Early and accessible diagnosis at the point of care, especially in limited-resource countries, represents the first step of clinical interventions. To overcome these limitations, we have proposed an innovative, sustainable paper-based electrochemical detection platform for thrombin. In this work, a sustainable paper-based aptasensor was rationally designed, characterized, evaluated against conventional gold standard plastic-based substrates, and applied to human serum, yielding a detection limit of ~ 60 pM. The present method provides an efficient and user-friendly way for the detection of thrombin and potentially leading to better management and treatment outcomes for patients
The very short story in the time of revolution. al-Mihmāz (the Spur) and the Syrian author Zakariyā Tāmir
No full textThe mass protests swept through the Middle East in early 2011 underlined the role of modern information-communication technologies (ICT). From a literary point of view, the “Arab Spring” inevitably marked the birth of a new model of writing, characterised by a more participatory, global and immediate manner of expression that could be defined as Humanism 2.0. In this context, we may insert the experimental writing by the famous Syrian author Zakariyā Tāmir: on the al-Mihmāz (The Spur) Facebook page the writer begins a literary journey publishing daily posts and explicitly supporting the Syrian revolution. This contribution intends to analyse a few of Tāmir’s most significant posts published on Facebook. The time span is 2012, just one year after the Syrian revolution: thanks to aphorisms, posts and short stories, a new literary pact with potential readers is inaugurated, within a phenomenon that we can call al-adab al-raqmī (digital literature
Introduction a "Iraq After 2003: When Trauma Becomes Art. Myth, History, and Literature"
No full textThe article aims at introducing the volume "Iraq After 2003: When Trauma Becomes Art. Myth, History, and Literature", edited By Ada Barbaro. The volume aims to provide lenses that examine, from various angles, a country, Iraq, in the aftermath of the quintessential trauma of its contemporary history, namely, 2003.The volume is thus a collection of "narratives", of
narrative acts that render the relationship between reality and its discourse almost oxymoronic. The present work, born in the wake of the conference «Iraq After 2003: When Trauma Becomes Art. Myth, History, and Literature», is therefore published as an outcome of the scientific project "Forms, Languages, and [Con]texts of Tàrìkh: Writing and Rewriting History in Iraq", of which the author of these pages is the Principal Investigator
Sensing at Your Fingertip: On-Glove Electrochemical Sensor for Copper Detection on Vine Leaves
No full textIntensive use of copper-based pesticides in agriculture can impact human health and biodiversity: the accumulation of this metal in soil negatively affects crop yields. There is growing interest in developing new tools that are capable of monitoring copper occurrence in agricultural contexts through the use of quick and user-friendly approaches for non-specialists, e.g., farmers. This work focuses on the development of a glove-based electrochemical sensor, enhanced with gold nanoparticles, for the detection of copper ions on leaves. The developed device proved was capable of detecting copper ions contained in a copper-based pesticide commonly used in agriculture (Cupravit Bio Advanced). The on-glove analytical device was characterized using garden leaf as the model system and subsequently applied to on-site detection of copper ions on vine leaf treated with Cupravit Bio Advanced. The procedure was very facile: sampling was carried out by touching the leaf with the strip and the stripping-voltammetric measurement was performed by adding a few microliters of an acidic solution to the strip. The on-glove approach allowed evaluation of the level of copper-based pesticide used, avoiding complex and time-consuming tasks. Such operation opens up a wide range of possibilities for improving precision agriculture and sustainable development at the point-of-need for the use of non-specialists
Printed Electrochemical Strip for the Detection of miRNA-29a: A Possible Biomarker Related to Alzheimer's Disease
No full textThe development of electrochemical strips, as extremely powerful diagnostic tools, has received much attention in the field of sensor analysis and, in particular, the detection of nucleic acids in complex matrixes is a hot topic in the electroanalytical area, especially when directed toward the development of emerging technologies, for the purpose of facilitating personal healthcare. One of the major diseases for which early diagnosis is crucial is represented by Alzheimer's disease (AD). AD is a progressive neurodegenerative disease, and it is the most common cause of dementia worldwide. In this context microRNAs (miRNAs), which are small noncoding RNAs, have recently been highlighted for their promising role as biomarkers for early diagnosis. In particular, miRNA-29 represents a class of miRNAs known to regulate pathogenesis of AD. In this work we developed an electrochemical printed strip for the detection of miRNA-29a at low levels. The architecture was characterized by the presence of gold nanoparticles (AuNPs) and an anti-miRNA-29a probe labeled with a redox mediator. The novel analytical tool has been characterized with microscale thermophoresis and electrochemical methods, and it has been optimized by selection of the most appropriate probe density to detect low target concentration. The present tool was capable to detect miRNA-29a both in standard solution and in serum, respectively, down to 0.15 and 0.2 nM. The platform highlighted good repeatability (calculated as the relative standard deviation) of ca. 10% and satisfactory selectivity in the presence of interfering species. This work has the objective to open a way for the study and possible early diagnosis of a physically and socially devastating disease such as Alzheimer's. The results demonstrate the suitability of this approach in terms of ease of use, time of production, sensitivity, and applicability
Technical Evaluation of a Paper-Based Electrochemical Strip to Measure Nitrite Ions in the Forensic Field
No full textNitrite is a compound used as a food additive for its preservative action and coloring capability, as well as an industrial agent for its antifreezing action and for preventing corrosion, and it is also used as a pharmaceutical in cyanide detoxification therapy. However, even recently, because of its high toxicity, it has been used as a murder and suicidal agent due to its affordability and ready availability. In this technical report, we describe an electrochemical paper-based device for selectively determining nitrite in complex biofluids, such as blood, cadaveric blood, vitreous humor, serum, plasma, and urine. The approach was validated in terms of the linearity of response, selectivity, and sensitivity, and the accuracy of the determination was verified by comparing the results with a chromatographic instrumental method. A linear response was observed in the micromolar range; the sensitivity of the method expressed as the limit of detection was 0.4 μM in buffer measurements. The simplicity of use, the portability of the device, and the performance shown make the approach suitable for detecting nitrite in complex biofluids, including contexts of forensic interest, such as murders or suicides in which nitrite is used as a toxic agent. Limits of detection of ca. 1, 2, 4, 5, 3, and 4 μM were obtained in vitreous humor, urine, serum and plasma, blood, and cadaveric blood, also highlighting a satisfactory accuracy comprised between 91 and 112%
Il culto di san Michele in Campania. Antonino e Catello
No full textIl volume, diviso in quattro sezioni, approfondisce l'origine e la diffusione del culto di San Michele in Campania, nelle sue varie espressioni. Nella prima parte vengono indagati gli insediamenti micaelici, con particolare riferimento alle numerose grotte che, sull'esempio di quella garganica, furono dedicate a San Michele soprattutto lungo l'asse Salerno-Avellino- Benevento. Il volume analizza numerosi testi agiografici campani che attestano la presenza dell'Angelo talvolta con generiche funzioni di angelo custode, talaltra con gli attributi tipici di San Michele. Una cospicua sezione del volume è dedicata al dossier agiografico di Antonino e Catello, rispettivamente monaco e vescovo di Sorrento e Stabia, un'area da sempre roccaforte bizantina. Sulla base di elementi storici e di elementi rivenienti dal dossier agiografico viene posticipata all'XI secolo - e quindi di oltre un secolo rispetto alle posizioni della critica- l'epoca di composizione della Vita Antonini (XI secolo). In quest'epoca Sorrento vive un particolare momento di vivacità e di rinnovamento a livello politico, sociale e religioso. Nei testi agiografici Antonino e Catello sono co-fondatori del Santuario del Monte Faito, considerato il santuario nazionale dei Bizantini, anche per ragioni di antagonismo e rivalità con il Santuario di San Michele del Gargano, santuario nazionale dei LongobardiThe book, divided into four parts, is a thorough analysis of the cult of St. Michael in Campania, in its various expressions.
In the first part the author presents micaelici settlements with particular reference to the numerous caves that, based on the example of the Gargano, were dedicated to the Angel, especially along the road Salerno-Avellino-Benevento. The book contains an examination of the hagiographic texts from Campania attesting the presence of Angel, sometimes in its generic function of custos, sometimes with attributes typical of Michael.
The author analyzes the hagiographic dossier of Antoninus and Catello, respectively monaco and bishop of Sorrento and Stabiae. The author basing on historical data and data emerging from the same dossier, delaies more than a century the composition of the Vita Antonini (11th century). In this century Sorrento lived a revival on political-institutional-religious level. The anonymous author of Life - probably a monaco /bishop - approaches Antonino (IX century) and Catello (sixth century) making the abbot of Sorrento co-starred in the foundation of the sanctuary of St. Michael on Mount Fait, considered a national shrine of the Byzantines in Naples, also for reasons of competition with the sanctuary of Mount St. Michael Gargano, traditionally linked to the Lombards
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