2 research outputs found
Isolation of Food-Borne Microorganisms from Atlantic Mackerel and Disinfection of the Raw Fish by Radiation, Low Temperature and Combination Treatments
No full textAn investigation was undertaken for the isolation and identification of fish-borne microorganisms from mackerel fish (Scomberomorus guttatus) collected from Savar Bazar. Radiation, low temperature (-20°C) and combination treatments were then applied for the decontamination of associated organisms. The ranges of total viable bacterial count, total coliform count, total faecal coliform count and total staphylococcal count varied from 6.5 x 104 to 1.04 x 105, 2 x 102 to 4.0 x 102, 0 to 1 x 102 and 4.4 x 104 to 3.8 x 104 cfu/g respectively, while the total fungal count was nil. Sixty-four bacterial isolates were identified including Staphylococcus (19%), Micrococcus (11%), Enterobacter (8%), Klebsiella (17%), Bacillus (19%), Escherichia (17%) and Pseudomonas (9%). To disinfect the microorganisms, the samples were irradiated at different doses (0-10.0 kGy) of gamma irradiation. Total coliform and total faecal coliform bacteria were inactivated below the detectable level at 2.5 kGy of irradiation. The number of total viable bacteria decreased by three logs when irradiated at 5.0 kGy and the number of Staphylococcus bacteria were eliminated completely after the irradiation dose of 5.0 kGy. During frozen storage, the total viable bacterial counts were gradually declined in all the samples. After six months of storage the bacterial counts were decreased about one log in all of the samples. It has been observed that combination treatments (irradiation and freezing) are more effective than the single treatment for eliminating the fish-borne bacteria. Keywords: Mackerel fish; Microbial contamination; Irradiation; Low temperatureDOI: http://dx.doi.org/10.3329/bjm.v25i2.4871 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 105-109</jats:p
Bacteriological profile and antimicrobial susceptibility patterns of symptomatic bloodstream infection in Dhaka City
No full textINTRODUCTION: Bacteremia, is regarded as a significant reason in all over the world for morbidity and mortality in mainly sick patients. Invasion by microorganisms in blood constitutes the critical issues in infectious disease. The aims and objectives are to find out the pathogenic agents infecting males and females in blood of different age groups and naturally used antimicrobial sensitivity profiles. METHODS: All the blood samples were collected aseptically and tested in Popular Diagnostic Centre, Dhaka, Bangladesh between May, 2020 and October, 2020. Total 305 blood specimens from both sexes of different age groups were collected. Cultural analysis was performed by Gram staining, phenotypic examination, biochemical and serological analysis. Finally, antibiotic susceptibility test was done against commonly used antibiotics. RESULTS: 96 (31.47%) samples yielded a positive culture with predominant isolate being Salmonella typhi (41.66%) though Salmonella spp., Salmonella typhi, Staphylococcus spp and Pseudomonas spp. were also detected. Like most of previous reports, Salmonella spp was predominant, this corroborates this study. But the profile of antimicrobial susceptibility of the detected organisms varied comparing studies which were done in the past. The isolates were found mostly resistant to nalidixic acid. Most of the pathogens showed tremendous susceptibility against ceftriaxone, cefixime, ceftazidime etc.
CONCLUSION: The antibiotic selection for the treatment of bacteraemia in patients should always be serious concern due to multidrug resistant (MRD) bacterial isolates. For proper treatment of anti-bacterial resistance and critical mortality and morbidity should be related with the sickness. For validating more reliability, this research requires further work
