3 research outputs found
Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii
No full textMastan, Anthati, Rane, Digeshwar, Dastager, Syed G., Babu, C.S. Vivek (2021): Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii. Phytochemistry (112654) 184: 1-10, DOI: 10.1016/j.phytochem.2021.112654, URL: http://dx.doi.org/10.1016/j.phytochem.2021.11265
Trichoderma viride
No full text2.2. Synergistic effect of fungal endophytes and T. viride on plant growth of C. forskohlii Co-inoculation of RF 1, SF1, or SF2 fungal endophyte with T. viride modulates C. forskohlii plant growth. Significant enhancement in branch number and plant height was noticed in both individual (endophyte or T. viride) and co-inoculated treatments (endophyte +TV1) compared to the uninoculated control plants. However, more enhancements in plant height and branch numbers were noticed in co-inoculations compared to individual treatments (Fig. S2). C. forskohlii treated with RF 1, SF1, SF2, and TV1 showed 29–54% and 21–33% more branch number and height, respectively, compared to control plants. The plants co-inoculated with RF 1+TV1, SF1+TV1, and SF2+TV1 resulted in significant improvement of plant height (54, 41, and 52%, respectively) and number of branches (75, 67, and 70%, respectively) compared to control plants (Fig. 2a and b). Fresh and dry mass of root and shoot were significantly higher in co-inoculated plants compared to individual treatments and control plants. However, individual treatments showed more fresh and dry mass of root and shoot compared to control plants. The plants inoculated with RF 1+TV1, SF1+TV1, and SF2+TV1 resulted in significant increase of fresh shoot weight at 100, 70, and 97%, fresh root weight at 73, 44, and 55%, dry shoot weight at 67, 32, and 56%, and dry root weight at 94, 53, and 69%, respectively compared to control plants. Similarly, treatment of C. forskohlii with RF 1, SF2, and TV1 resulted in significant enhancement of root length (20, 27, and 37%, respectively) and number of tuberous roots (37, 27, 24, and 20%, respectively) compared to control plants (Fig. 2c–f). However, SF1 did not significantly enhance root length (7%). The plants treated with a consortium of RF 1+TV1, SF1+TV1, or SF2+TV1 resulted in significant increment of root length (41, 16, or 37%, respectively) and number of tuberous roots (74, 35, or 55%, respectively) compared to control plants (Fig. 2g and h). Overall, the effect of endophytes and TV 1 in different combinations (individual and co-inoculation) revealed that the inoculation of RF 1+TV1 combination showed significant improvement in all growth measurements, followed by combination of SF2+TV1 compared to other treatments and control plants.Published as part of Mastan, Anthati, Rane, Digeshwar, Dastager, Syed G. & Babu, C. S. Vivek, 2021, Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii, pp. 1-10 in Phytochemistry (112654) (112654) 184 on page 2, DOI: 10.1016/j.phytochem.2021.112654, http://zenodo.org/record/829227
Trichoderma viride
No full text5.3. Co-inoculation of endophytic fungi with Trichoderma viride Prior to co-inoculation studies, compatibility between each fungal endophyte and T. viride was tested using dual culture method and found no antagonism between endophytes and T. viride. Further, co-inoculation studies were carried out under field conditions at CSIR–CIMAP Research Centre, Bangalore in an experimental plot (3 × 3 m) of red-sandy loom soil (pH 6.2). Thirty-days-old nursery plants of C. forskohlii were transplanted into 10–12 cm deep holes with spacing of 60 × 60 cm between plants. Four rows were maintained in each treatment plot (total of 8 plots) and divided by a guard ridge (50 cm width) to avoid direct contact with adjacent plots. Each experimental plot was planted with 16 plants in four rows (hence, 4 plants were in the middle surrounded by 12 peripheral plants). The four central plants (four replicates) from each treatment were selected for the measurement of growth parameters. The fungal cultures were grown in 500 mL potato dextrose broth for 7 days at 28 ◦ C and the resulting mycelia were diluted with 90 mL of phosphate buffered saline (PBS) (K2HPO4 0.24 g L 1, Na2HPO4 1.44 g L 1, KCl 0.2 g L 1, NaCl 8 g L 1 and pH 7.40). Finally, fungal suspension was diluted to a density of 1 × 108 CFU mL 1. After 30 days of transplantation, each plant in the experimental plot was treated with 30 mL inoculum of SF1, SF2, RF 1 or TV1 and the untreated experimental plot (n = 16 plants) was considered as control. Co-inoculation treatments included RF 1+TV1, SF1+TV1, and SF2+TV1 at an inoculum of 1:1 ratio (Egamberdieva et al., 2017). For each treatment plot, plant growth measurements comprising number of branches and plant height were analyzed for every 30 days until harvest. After 150-days of transplantation, plants were harvested by manually uprooting each plant with utmost care so as to not damage the roots. Fresh weights of roots and shoots were measured at the time of harvest and dry weights of roots and shoots were measured after 4-weeks of drying period; root length and number of tuberous roots per plant were also recorded. The obtained values of endophyte treatments (alone and co-inoculum) were compared with endophyte uninoculated control plants.Published as part of Mastan, Anthati, Rane, Digeshwar, Dastager, Syed G. & Babu, C. S. Vivek, 2021, Molecular insights of fungal endophyte co-inoculation with Trichoderma viride for the augmentation of forskolin biosynthesis in Coleus forskohlii, pp. 1-10 in Phytochemistry (112654) (112654) 184 on page 8, DOI: 10.1016/j.phytochem.2021.112654, http://zenodo.org/record/829227
