323,797 research outputs found
Randel (William Peirce) Papers, 1940-1992
No full textThis record group contains the papers of William Peirce Randel, a professor of English at the University of Maine, born on January 7, 1909, in New York City. Papers include manuscripts for various books, articles, and talks authored by Randel. Also, includes correspondence, research materials, drafts of articles, and copies of Maine legislative documents concerning higher education. The correspondence, dates primarily from 1962-1992, and included both incoming letters and copies of outgoing letters involving various Maine politicians, especially William S. Cohen. The correspondence concerns current events of the time including higher education, world affairs, and issues of aging.https://digitalcommons.library.umaine.edu/findingaids/1443/thumbnail.jp
RanDeL-Seq: a High-Throughput Method to Map Viral cis- and trans-Acting Elements
No full textIt has long been known that noncoding genomic regions can be obligate cis elements acted upon in trans by gene products. In viruses, cis elements regulate gene expression, encapsidation, and other maturation processes, but mapping these elements relies on targeted iterative deletion or laborious prospecting for rare spontaneously occurring mutants. Here, we introduce a method to comprehensively map viral cis and trans elements at single-nucleotide resolution by high-throughput random deletion. Variable-size deletions are randomly generated by transposon integration, excision, and exonuclease chewback and then barcoded for tracking via sequencing (i.e., random deletion library sequencing [RanDeL-seq]). Using RanDeL-seq, we generated and screened >23,000 HIV-1 variants to generate a single-base resolution map of HIV-1's cis and trans elements. The resulting landscape recapitulated HIV-1's known cis-acting elements (i.e., long terminal repeat [LTR], Ψ, and Rev response element [RRE]) and, surprisingly, indicated that HIV-1's central DNA flap (i.e., central polypurine tract [cPPT] to central termination sequence [CTS]) is as critical as the LTR, Ψ, and RRE for long-term passage. Strikingly, RanDeL-seq identified a previously unreported ∼300-bp region downstream of RRE extending to splice acceptor 7 that is equally critical for sustained viral passage. RanDeL-seq was also used to construct and screen a library of >90,000 variants of Zika virus (ZIKV). Unexpectedly, RanDeL-seq indicated that ZIKV's cis-acting regions are larger than the untranscribed (UTR) termini, encompassing a large fraction of the nonstructural genes. Collectively, RanDeL-seq provides a versatile framework for generating viral deletion mutants, enabling discovery of replication mechanisms and development of novel antiviral therapeutics, particularly for emerging viral infections.IMPORTANCE Recent studies have renewed interest in developing novel antiviral therapeutics and vaccines based on defective interfering particles (DIPs)-a subset of viral deletion mutants that conditionally replicate. Identifying and engineering DIPs require that viral cis- and trans-acting elements be accurately mapped. Here, we introduce a high-throughput method (random deletion library sequencing [RanDeL-seq]) to comprehensively map cis- and trans-acting elements within a viral genome. RanDeL-seq identified essential cis elements in HIV, including the obligate nature of the once-controversial viral central polypurine tract (cPPT), and identified a new cis region proximal to the Rev responsive element (RRE). RanDeL-seq also identified regions of Zika virus required for replication and packaging. RanDeL-seq is a versatile and comprehensive technique to rapidly map cis and trans regions of a genome
Statistische Übersicht Der Vornehmsten Deutschen Und Sämmtlichen Europäischen Staaten : In Ansehung Ihrer Grösse, Bevölkerung, Ihres Finanz- Und Kriegeszustandes ; Mit Einem Anhang Gleichen Inhalts Über Den Nordamerikanischen Frey-Staat
No full text[Johann Adolf Friedrich Randel]Vorlageform des Erscheinungsvermerks: 1786. - Erscheinungsort und Verlag ermittelt in: GB 1700/1910, Bd. 148, S. 18
480km transmission of MDM 576-Gb/s 8QAM using a few-mode re-circulating loop
No full textWe demonstrate successful 3-mode-division-multiplexed × 192-Gb/s dual-polarization 8QAM (total 576 Gb/s) transmission over 480 km of few-mode fiber (FMF). This distance was obtained using an all few-mode re-circulating loop containing a 60 km FMF span
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RanDeL-Seq: a High-Throughput Method to Map Viral cis- andtrans-Acting Elements
No full textIt has long been known that noncoding genomic regions can be obligate
cis elements acted upon in trans by gene products. In viruses, cis
elements regulate gene expression, encapsidation, and other maturation
processes, but mapping these elements relies on targeted iterative
deletion or laborious prospecting for rare spontaneously occurring
mutants. Here, we introduce a method to comprehensively map viral cis
and trans elements at single-nucleotide resolution by high-throughput
random deletion. Variable-size deletions are randomly generated by
transposon integration, excision, and exonuclease chewback and then
barcoded for tracking via sequencing (i.e., random deletion library
sequencing [RanDeL-seq]). Using RanDeL-seq, we generated and screened
>23,000 HIV-1 variants to generate a single-base resolution map of
HIV-1’s cis and trans elements. The resulting landscape recapitulated
HIV-1’s known cis-acting elements (i.e., long terminal repeat [LTR],
W, and Rev response element [RRE]) and, surprisingly, indicated that
HIV-1’s central DNA flap (i.e., central polypurine tract [cPPT] to
central termination sequence [CTS]) is as critical as the LTR, W, and
RRE for long-term passage. Strikingly, RanDeL-seq identified a
previously unreported similar to 300-bp region downstream of RRE
extending to splice acceptor 7 that is equally critical for sustained
viral passage. RanDeL-seq was also used to construct and screen a
library of >90,000 variants of Zika virus (ZIKV). Unexpectedly,
RanDeL-seq indicated that ZIKV’s cis-acting regions are larger than the
untranscribed (UTR) termini, encompassing a large fraction of the
nonstructural genes. Collectively, RanDeL-seq provides a versatile
framework for generating viral deletion mutants, enabling discovery of
replication mechanisms and development of novel antiviral therapeutics,
particularly for emerging viral infections.
IMPORTANCE Recent studies have renewed interest in developing novel
antiviral therapeutics and vaccines based on defective interfering
particles (DIPs)-a subset of viral deletion mutants that conditionally
replicate. Identifying and engineering DIPs require that viral cis- and
trans-acting elements be accurately mapped. Here, we introduce a
high-throughput method (random deletion library sequencing
[RanDeL-seq]) to comprehensively map cis- and trans-acting elements
within a viral genome. RanDeL-seq identified essential cis elements in
HIV, including the obligate nature of the once-controversial viral
central polypurine tract (cPPT), and identified a new cis region
proximal to the Rev responsive element (RRE). RanDeL-seq also identified
regions of Zika virus required for replication and packaging. RanDeL-seq
is a versatile and comprehensive technique to rapidly map cis and trans
regions of a genome
Discrete Multitone Modulation for Maximizing Transmission Rate in Step-Index Plastic Optical Fibres
No full textThe use of standard 1-mm core-diameter step-index plastic optical fiber (SI-POF) has so far been mainly limited to distances of up to 100 m and bit-rates in the order of 100 Mbit/s. By use of digital signal processing, transmission performance of such optical links can be improved. Among the different technical solutions proposed, a promising one is based on the use of discrete multitone (DMT) modulation, directly applied to intensity-modulated, direct detection (IM/DD) SI-POF links. This paper presents an overview of DMT over SI-POF and demonstrates how DMT can be used to improve transmission rate in such IM/DD systems. The achievable capacity of an SI-POF channel is first analyzed theoretically and then validated by experimental results. Additionally, first experimental demonstrations of a real-time DMT over SI-POF system are presented and discusse
Author response
No full textDevelopmental programs have the fidelity to form neural circuits with the same structure and function among individuals of the same species. It is less well understood, however, to what extent entire neural circuits of different individuals are similar. Previously, we reported the neuronal connectome of the visual eye circuit from the head of a Platynereis dumerilii larva (Randel et al., 2014). We now report a full-body serial section transmission electron microscopy (ssTEM) dataset of another larva of the same age, for which we describe the connectome of the visual eyes and the larval eyespots. Anatomical comparisons and quantitative analyses of the two circuits reveal a high inter-individual stereotypy of the cell complement, neuronal projections, and synaptic connectivity, including the left-right asymmetry in the connectivity of some neurons. Our work shows the extent to which the eye circuitry in Platynereis larvae is hard-wired
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Thermal structure of intense convective clouds derived from GPS radio occultations
No full textThermal structure associated with deep convective clouds is investigated using Global Positioning System (GPS) radio occultation measurements. GPS data are insensitive to the presence of clouds, and provide high vertical resolution and high accuracy measurements to identify associated temperature behavior. Deep convective systems are identified using International Satellite Cloud Climatology Project (ISCCP) satellite data, and cloud tops are accurately measured using Cloud-Aerosol Lidar with Orthogonal Polarization (CALIPSO) lidar observations; we focus on 53 cases of near-coincident GPS occultations with CALIPSO profiles over deep convection. Results show a sharp spike in GPS bending angle highly correlated to the top of the clouds, corresponding to anomalously cold temperatures within the clouds. Above the clouds the temperatures return to background conditions, and there is a strong inversion at cloud top. For cloud tops below 14 km, the temperature lapse rate within the cloud often approaches a moist adiabat, consistent with rapid undiluted ascent within the convective systems
Recent Results From the EU POF-PLUS Project: Multi-Gigabit Transmission Over 1 mm Core Diameter Plastic Optical Fibers
No full textRecent activity to achieve multi-gigabit transmission over 1 mm core diameter graded-index and step-index plastic optical fibers for distances up to 50 meters is reported in this paper. By employing a simple intensity-modulated direct-detection system with pulse amplitude or digital multi-tone modulation techniques, low-cost transceivers and easy to install large-core POFs, it is demonstrated that multi-gigabit transmission up to 10 Gbit/s over 1-mm core diameter POF infrastructure is feasible. The results presented in this paper were obtained in the EU FP7 POF-PLUS project, which focused on applications in different scenarios, such as in next-generation in-building residential networks and in datacom applications
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