32 research outputs found
Study of phenotypic and gene expression response to bacterial challenge
No full textIn the mouse model of Bacillus Calmette-Guérin (BCG)-induced inflammation, focus is placed upon phenotypic and gene-expression responses to the original challenge. Studies of the phenotypic response to BCG involves several behavior indicators, categorized as representing an initial period of sickness response, and a set of depression-like behaviors that can endure for several weeks. Little is known about the impact of relationships among indicators on these studies, as most analyses have treated indicators independently. Gene-expression studies have also been small in scale, limiting what is known about gene-expression during these behavioral changes. With the availability of Next-Generation Sequencing platforms, the scale of transcriptome analysis can be greatly increased. This study aims to address these previous limitations, characterizing behavioral and gene-expression responses to BCG-induced inflammation.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-05-01The student, Scott Nixon, accepted the attached license on 2015-04-21 at 11:27.The student, Scott Nixon, submitted this Dissertation for approval on 2015-04-21 at 15:53.This Dissertation was approved for publication on 2015-04-23 at 09:47.DSpace SAF Submission Ingestion Package generated from Vireo submission #7977 on 2015-07-22 at 14:25:57Made available in DSpace on 2015-07-22T22:45:44Z (GMT). No. of bitstreams: 5
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Previous issue date: 2015-04-23Embargo set by: Seth Robbins for item 80004
Lift date: 2017-07-22T22:46:21Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 80004 on 2017-07-23T09:15:27Z
FORMULACIÓN DE UN BANCO DE MASAS MADRE A BASE DE ELEMENTOS AGRÍCOLAS DEL DEPARTAMENTO PARA CREAR NUEVOS PRODUCTOS DE PANADERÍA
No full textSourdough is a natural culture of micro-organisms, mainly yeasts and lactic acid bacteria, used in the fermentation of bakery products. It is composed of a dynamic and complex mixture of these microorganisms, where yeasts, such as Saccharomyces cerevisiae, and lactic acid bacteria, such as Lactobacillus and Leuconostoc, play key roles in the fermentation process (Gobbetti, 1998; De Vuyst and Neysens, 2005).It is made from a mixture of flour and water that has been fermented by naturally occurring yeasts and bacteria present in the environment. It is used as a leavening agent in bread making, providing flavour, aroma and improving the texture of the bread. In the research carried out in this project, 4 types of sourdough doughs different from the one made with wheat flour were produced, using starchy agricultural products from the region, in this case maize, banana and cassava. The sourdough bank is composed of the flours produced within the project and with other wheat flour flours that have been produced since 2020. La masa madre es un cultivo natural de microorganismos, principalmente levaduras y bacterias lácticas, que se utiliza en la fermentación de productos de panificación. Se compone de una mezcla dinámica y compleja de estos microorganismos, donde las levaduras, como Saccharomyces cerevisiae, y las bacterias lácticas, como Lactobacillus y Leuconostoc, desempeñan roles clave en el proceso de fermentación (Gobbetti, 1998; De Vuyst y Neysens, 2005).Se fabrica con una mezcla de harina y agua que ha sido fermentada por levaduras y bacterias naturales presentes en el ambiente. Se utiliza como agente fermentador en la elaboración del an, proporcionando sabor, aroma y mejorando la textura del pan. En la investigación que se hizo en este proyecto se logró elaborar 4 tipos de masas madres diferentes a la elaborada con harina de trigo, se tomaron productos agrícolas feculentos de la región, en este caso maíz, plátano yuca, se hizo la extracción de harinas y con esta se crearon las masas madres. El banco de masas madres está compuesta por las harinas elaboradas dentro del proyecto y con otras de harina de trigo que estas elaboradas desde el 2020. 
Fortalecimiento de los estilos de aprendizaje, mediante el desarrollo de software educativo multimedia en la asignatura de Estudios Sociales para la Unidad Educativa Carlos Cisneros
No full textSe ha implementado un software educativo llamado “Culturas Americanas”, el cual presenta un
ambiente acogedor y llamativo para facilitar el desenvolvimiento de los estudiantes, fue
aplicado en el noveno grado de la Unidad Educativa “Carlos Cisneros” de la ciudad de
Riobamba, esta es una investigación de campo en primer lugar utilizamos el cuestionario
CHAEA, para descubrir los estilos de aprendizaje, predominantes en los novenos grados
paralelos A,B y C utilizando el 100% de la población existente, los estilos de aprendizaje que
fueron tomados para esta tesis son los descritos por Peter Honey y Alan Mumford en 1988,
90
90
siendo estos los estilos activo, reflexivo, teórico, y el pragmático, para desarrollar el software
educativo se utilizó las fases descritas por las autoras Isabel Ogalde y Maricarmen Rodríguez,
el software de autor utilizado fue Atenex Constructor versión 4.3, para la asignatura de
Estudios Sociales durante el primer quimestre del año lectivo 2014 -2015, logrando con este
software mejorar la motivación, la interacción y sobre todo, incrementando en un 15% todos los
estilos de aprendizaje, por tanto se recomienda a las autoridades y demás docentes utilizar este
software educativo tanto en nuestra institución, como en otras instituciones para mejorar el
aprendizaje de Estudios Sociales.We have implemented an educational software called "American Cultures", which provides a
welcoming and appealing environment to facilitate the development of students, was applied in
the ninth grade of the Education Unit "Carlos Cisneros" of the city of Riobamba, this is field
research first used the CHAEA questionnaire to discover the learning styles prevalent in the
ninth parallel grades A, B and C using 100% of the existing population, learning styles that
were taken for this thesis are described by Peter Honey and Alan Mumford in 1988, which are
the active, reflexive, theoretical styles, and pragmatic, to develop educational software the
phases described by the authors Isabel Ogalde and Maricarmen Rodriguez, author used the
software used was Atenex Builder version 4.3, for the subject of Social Studies in the first
quimestre the 2014 -2015 school year, making this software improve motivation, interaction and
above all, increasing by 15% every learning style therefore is recommends the authorities and
other teachers use this software in both our educational institution and other institutions to
enhance learning of social studies
The atmosphere of Titan in late northern summer from JWST and Keck observations
No full textNixon, Conor A. et al.-- Full list of auhtors: Nixon, Conor A.; Bézard, Bruno; Cornet, Thomas; Coy, Brandon Park; de Pater, Imke; Es-Sayeh, Maël; Hammel, Heidi B.; Lellouch, Emmanuel; Lombardo, Nicholas A.; López-Puertas, Manuel; Lora, Juan M.; Rannou, Pascal; Rodriguez, Sébastien; Teanby, Nicholas A.; Turtle, Elizabeth P.; Achterberg, Richard K.; Alvarez, Carlos; Davies, Ashley G.; de Kleer, Katherine; Doppmann, Greg; Fletcher, Leigh N.; Hayes, Alexander G.; Holler, Bryan J.; Irwin, Patrick G. J.; Jordan, Carolyn; King, Oliver R. T.; Kutsop, Nicholas W.; Marlin, Theresa C.; Melin, Henrik; Milam, Stefanie N.; Molter, Edward M.; Moore, Luke; Nyffenegger-Péré, Yaniss; O'Donoghue, James; O'Meara, John; Rafkin, Scot C. R.; Roman, Michael T.; Rostopchina, Arina; Rowe-Gurney, Naomi; Schmidt, Carl; Schmidt, Judy; Sotin, Christophe; Stallard, Tom S.; Stansberry, John A.; West, Robert A.Saturn's moon Titan undergoes a long annual cycle of 29.45 Earth years. Titan's northern winter and spring were investigated in detail by the Cassini-Huygens spacecraft (2004-2017), but the northern summer season remains sparsely studied. Here we present new observations from the James Webb Space Telescope (JWST) and Keck II telescope made in 2022 and 2023 during Titan's late northern summer. Using JWST's mid-infrared instrument, we spectroscopically detected the methyl radical, the primary product of methane break-up and key to the formation of ethane and heavier molecules. Using the near-infrared spectrograph onboard JWST, we detected several non-local thermodynamic equilibrium CO and CO2 emission bands, which allowed us to measure these species over a wide altitude range. Lastly, using the near-infrared camera onboard JWST and Keck II, we imaged northern hemisphere tropospheric clouds evolving in altitude, which provided new insights and constraints on seasonal convection patterns. These observations pave the way for new observations and modelling of Titan's climate and meteorology as it progresses through the northern fall equinox, when its atmosphere is expected to show notable seasonal changes.C.A.N. was funded for this work by JWST Archive Research Project 02524. B.B., E.L., P.R., S.R. and M.E.-S. acknowledge support from the Programme National de Planétologie of CNRS-INSU co-funded by CNES. S.R. also acknowledges financial support from the CNES and the French National Research Agency (grant nos. ANR-21-CE49-0020-04/RAD3-NET and ANR-23-CE56-0008/EOLE). M.L.-P. acknowledges financial support from the Agencia Estatal de Investigación, MCIN/AEI/10.13039/501100011033 (grant nos. PID2022-141216NB-I00 and CEX2021-001131-S). N.A.T. was funded by the UK Science and Technology Facilities Council (grant no. ST/Y000676/1). N.A.L. and J.M.L. were funded by NASA CDAP (grant no. 80NSSC20K0483). H.B.H. and S.N.M. acknowledge support from NASA JWST Interdisciplinary Scientist grant 21-SMDSS21-0013. H.M. was supported by the STFC James Webb Fellowship (ST/W001527/2) at Northumbria University. LNF, ORTK and MTR were supported by STFC Consolidated Grant reference ST/W00089X/1. For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to the Author Accepted Manuscript version arising from this submission. A portion of this work used the ALICE high performance computing facility at the University of Leicester. This work is based in part on observations made with the NASA/ESA/CSA JWST. The data were obtained from the Mikulski Archive for Space Telescopes at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-03127 for JWST. These observations are associated with programme 1251. We thank the following staff at the Space Telescope Institute for support with the execution of the JWST observations: K. Murray, B. Hilbert, G. Wahlgren and B. Porterfield. Some of the data presented in this paper were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California and NASA. Observing time for this project was allocated by all three institutions, in part thanks to the Twilight Zone Program. NASA Keck time is administered by the NASA Exoplanet Science Institute. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation. We recognize and acknowledge the important cultural role and reverence that the summit of Maunakea has always had within the indigenous Hawaiian community. We are most fortunate to have had the opportunity to conduct observations from this mountain.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).Peer reviewe
Nurse safety: How is safety climate related to affect and attitude?
No full textfor nurses, given the hazardous healthcare environment. This study advances the research on workplace safety by studying the process variables (i.e. job-related negative affect (JRNA) and job satisfaction) in explaining the relationship between safety climate and various
safety criteria in nurses. Based on survey data from 326 nurses, our findings suggest that psychological safety climate is negatively related to JRNA, turnover intentions, safety workarounds, and workplace hazards. In addition, structural equation modelling indicated general support for a model in which psychological safety climate influences employee strain through job attitudes, including JRNA and job satisfaction. More specifically, job attitudes were found to mediate the relationship between psychological safety climate and turnover intentions,
experience of hazards, and injuries. Safety workarounds did not significantly relate to injuries. The present study contributes to the ongoing improvement of interventions aimed at mitigating nurses’ injuries by integrating job attitudes into the safety climate–safety outcome framework.Journal ArticleFinal article publishe
Prostaglandin E2 mediated inflammation and hypoxia in feline oral squamous cell carcinoma
No full textOral squamous cell carcinoma (OSCC) is the most common tumour of the feline oral cavity and is associated with devastating outcomes. Similar to human OSCC, Cyclooxygenase 2 (COX-2) expression and prostaglandin E2 (PGE2) synthesis have been demonstrated in feline OSCC (FOSCC). CD147, a known mediator of invasive behaviour, was also shown to be expressed in FOSCC. Other elements of the arachidonic acid pathway of inflammation, including microsomal and cytosolic PGE2 synthase enzymes (mPGES1, mPGES2 and cPGES) and PGE2 receptors (EP1-EP4), have not been investigated in FOSCC. Tumour hypoxia can support growing tumours by stimulating formation of new blood vessels through activity of hypoxia inducible factor 1 alpha (HIF-1α) and expression of vascular and endothelial growth factor type A (VEGFA). In humans, hypoxia impacts radiotherapy treatment and decreases the sensitivity of OSCC to chemotherapy. In human OSCC, expression of p16 is associated with papillomavirus infection and better prognosis. There have been fewer studies of p16 in FOSCC, and none directly comparing p16 expression in FOSCC to human OSCC. The objectives of this research were to investigate gene expression related to PGE2-associated inflammation and angiogenesis in vitro, to explore the relationship between mPGES1 and p16, COX-2, and CD147 in human and feline OSCC tumour samples, and to evaluate the effect of hypoxia on gene expression and cell culture viability resulting from chemotherapy and anti-inflammatory drug treatment. This work demonstrated that FOSCC cell lines express mPGES/cPGES genes (PTGES1-3) and EP receptor genes (PTGER1-4) to varying degrees, as well as genes encoding HIF-1α and VEGFA. Gene expression in FOSCC cells (SCCF2) was altered by exogenous PGE2 exposure, and EP4 antagonism resulted in varying responses suggesting that EP4 deserves further study in FOSCC. In biopsy samples of human and feline OSCC, immunohistochemistry (IHC) revealed that a subset of tumours expressed mPGES1 and p16. High CD147 expression was more common in high mPGES1 tumours in both species, though only reached statistical significance in the human samples. High p16 tumours were more common in the human oropharynx compared to the oral cavity, as expected given the importance of papillomavirus infection in the pathogenesis of human OSCC at this location. No significant difference in p16 expression between tongue and non-tongue tumours was observed in FOSCC. In vitro, 4 hours of hypoxia stimulated expression of PTGES1 (mPGES1). Interestingly, hypoxia reduced the sensitivity of SCCF2 to cytotoxic drugs (doxorubicin and carboplatin) but not to a cyclooxygenase inhibitor (piroxicam) or an EP4 inhibitor. Interestingly, hypoxia increased the antiproliferative effect of an mPGES1 inhibitor in SCCF2 cells. Collectively, these studies indicate mPGES1 may be an important mediator of inflammation and hypoxic response in FOSCC patients, possibly serving as a therapeutic target and deserving of continued study. Further study is needed in order to determine the role of p16 as a prognostic indicator in FOSCC. Based on p16 expression, it appears that FOSCC may have similarities with non-HPV-associated OSCC in people, which is more often localized to the oral cavity than the oropharynx and has a worse prognosis than HPV-associated tumours. New knowledge related to FOSCC mechanisms and treatment could benefit studies of oral cavity OSCC in people
A note on games corresponding to sequencing situations with due dates
No full textconvex cooperative games;one-machine sequencing situations;due dates;ready times
The safety and effectiveness of different methods of ear wax removal: a systematic review and economic evaluation
No full textEar wax (cerumen) is a natural secretion produced to protect the inner ear from dirt and other fragments by moving these particles towards the outer ear. If this process does not happen properly, wax may build up causing blockage in the ear canal and the possibility of impaction. People with a build up of ear wax may suffer from hearing loss, discomfort and, on occasions, infection. It may present problems in assessing hearing, blocking the view of the ear drum during medical examination and interfering with the fitting or function of hearing aids. Although it is thought to affect between 2% and 6% of the population in the England and Wales, some groups may be at a higher risk, such as those using hearing aids or with small ear canals and/or skin conditions. Recurrence is thought to be high among some of these groups. The consequences of the build up of ear wax in the ear canal are thought to be a common reason for consultation and cost in general practice with over 2 million consultations per year in the NHS.Methods of removal of ear wax include drops, flushing with water in general practice, and removal with suction or probes in specialist clinics. The relative safety and benefits of these different methods of removal remains uncertain. This research will systematically review published and unpublished evidence on the clinical and cost effectiveness of different methods for the removal of ear wax. Where appropriate, it will develop an economic model using data from this systematic review and other relevant sources to estimate the relative costs and benefits of different methods. In addition, the project will provide recommendations for future research to try to help answer any remaining areas of uncertainty
Formulación de un plan estratégico para la Empresa CG Ingenieros y Consultores S.A.S, mediante la metodología Balanced Scorecard
No full textTrabajo de investigaciónSe desarrolla un plan gerencial, para la empresa CG INGENIEROS Y CONSULTORES SAS, Analizando su estructura interna adicionalmente a esto se analizaron todos las factures externas que afectan a la empresa mediante un análisis tipo PESTEL; Adicionalmente se analizan y recopilan estos dos factores Internos y –Externos en una Matriz DOFA para finalmente poder plantar las estrategias que ayudaran a solucionar los problemas internos de la empresa.EspecializaciónEspecialista en Gerencia de Obras Civiles1. INTRODUCCIÓN
2. OBJETIVOS
3. MARCOS DE REFERENCIA
4. METODOLOGÍA
5. DIAGNOSTICO DE LA EMPRESA
6. PLANTEAMIENTO DE ESTRATEGIAS
7. MAPA ESTRATÉGICO
8. CUADRO DE MANDO INTEGRAL
9. CONCLUSIONES
RECOMENDACIONES
BIBLIOGRAFÍAS
ANEXO
Size at Sexual Maturity of Deep-Sea Unexploited Caribbean Metanephrops binghami (Boone, 1927) and Overexploited Mediterranean Nephrops norvegicus (Linnaeus, 1758) Using Morphometric and Gonadal Staging Approaches
No full text16 pages, 6 figures, 3 tables.-- Data Availability Statement: Data will be made available on requestThe deep-sea Caribbean lobster (Metanephrops binghami) and the Norway lobster (Nephrops norvegicus) are Nephropidae species of high commercial interest. Although the first one still remains unexploited, the second is overexploited in the Mediterranean Sea. For effective fisheries management, size at sexual maturity is an essential indicator to protect immature individuals from exploitation. The estimation of this indicator can, however, be biased due to the difficulty of differentiating juveniles from adults by their size structure due to the natural process of molting. This study aims to estimate the size at sexual maturity of M. binghami and N. norvegicus females by comparing the effectiveness of the morphometric method versus the macroscopic evaluation of gonad maturity. Samples of M. binghami were collected from the Colombian Caribbean Sea in August and December 2009, March and May 2010, and August 2020 to May 2021. Samples of N. norvegicus were collected from the northwestern Mediterranean Sea from 2019 to 2022. Similar sizes at sexual maturity were found for M. binghami between the morphometric approach (ranging from 28.6 to 33.9 mm cephalothorax length, CL) and the gonadal staging approach (31.4 mm CL). Conversely, for N. norvegicus, the morphometric approach yielded higher measurements (between 27.2 and 30.4 mm CL) than the gonadal approach (26.0 mm CL). This discrepancy might stem from the intense fishing overexploitation conditions of N. norvergicus, leading to a physiological adaptation that enables earlier gonadal maturation at faster rates than morphometric adaptation. Further research is required to elucidate these discrepancies and the effect of overexploitation on physiological (i.e., mature gonads) and functional maturity (i.e., capacity to brood eggs at a larger size)The second author (AR) was sponsored by the Fondo de Ciencia, Tecnología e Innovación (FCTeI) del Sistema General de Regalías (SGR) and the Doctoral Excellence Scholarship Program Bicentenario del Ministerio de Ciencia, Tecnología e Innovación (Minciencias). The scientific fishery sampling was funded by Ministerio de Ciencia, Tecnología e Innovación (Minciencias) (grant number 1117-452-21288) and Autoridad Nacional de Acuicultura y Pesca (AUNAP) and Universidad del Magdalena through cooperation agreement number 153-2020 under the research project “Reproductive biology of deep-sea crustaceans of commercial importance in the Colombian Caribbean”With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe
