36 research outputs found
Cells were labeled and invasion through chambers performed in the absence or presence of 10 nM EGF
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Shown are the results of one experiment performed in triplicate wells, which was repeated twice with similar results
Cells were cultured on Transwell inserts, and confocal immunofluorescence microscopy performed following incubation with primary antibodies against EGF-R (A), αintegrin (B) and βintegrin (C)
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Negative controls with primary antibody omitted did not show a detectable signal
Adhesion was tested under conditions of no growth factor (open symbols) and treatment with 10 nM EGF (closed symbols) for 8 hours
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Results are expressed as % adhesion ± SEM from three separate experiments, each performed in triplicate wells. * p < 0.003; ** p < 0.05
Cells were cultured on Transwell inserts, and confocal immunofluorescence microscopy performed following incubation with TRITC-conjugated phalloidin, in the absence (A) or presence (B) of 10 nM EGF
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Arrow shows focal area of actin staining consistent with a focal adhesion complex
Adhesion assays on collagen I using were performed with no growth factor (open bars), 10 nM EGF (closed bars) or 10 nM HRG-α (hatched bars)
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Shown are the results of three separate experiments, each performed in triplicate. * p < 0.001
Adhesion and invasion were measured following incubation with escalating doses of EGF
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Shown are the results of three experiments, each performed in triplicate
Adhesion assays on collagen I were performed with no growth factor (open bars), 10 nM EGF (closed bars) or 10 nM HRG-α (hatched bars)
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Shown are the results of three separate experiments, each performed in triplicate. * p < 0.001. **p < 0.02
Cells were cultured on invasion chambers coated with Matrigel, and invasion measured following treatment with no growth factor, 10 nM EGF, or 10 nM HRG-α
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Shown are the results of 3 experiments, each performed in triplicate wells. *p < 0.05 between the no growth factor group and the EGF treatment group for each cell type
The adhesion assay was performed and the experiment terminated at various time points
<p><b>Copyright information:</b></p><p>Taken from "Epidermal growth factor mediates detachment from and invasion through collagen I and Matrigel in Capan-1 pancreatic cancer cells"</p><p>BMC Gastroenterology 2005;5():12-12.</p><p>Published online 31 Mar 2005</p><p>PMCID:PMC1079814.</p><p>Copyright © 2005 Shirk and Kuver; licensee BioMed Central Ltd.</p> Adhesion was measured without and with EGF treatment. Shown are the results of one experiment performed in triplicate wells, which was repeated twice with similar results
Supersymmetry breaking in gauge theories and string theory
We study aspects of supersymmetry breaking in gauge theories and string theory. On the gauge theory side, we explore metastable vacua in a SQCD-like model with an extra sector connected by a singlet. The model combines dynamical supersymmetry breaking with an O'Raifeartaigh mechanism in terms of confined variables. On the string theory side, we study the dynamics of non-supersymmetric magnetized D-brane configurations on Calabi-Yau spaces. We also study the stabilization of the supersymmetry breaking runaway quiver.Ph.D.Includes bibliographical references (p. 161-174)
