40 research outputs found
Swelling e proprietà di rilascio di film edibili costituiti da purea di frutta e proteine della soia reticolate e non dall'enzima transglutaminasi
No full textMicrobiome signatures associated with flavor development differentiate Protected Designation of origin water Buffalo Mozzarella cheese from different production areas
No full textWater Buffalo Mozzarella (BM) is a typical cheese from Southern Italy with unique flavor profile and texture. It is
produced following a traditional back-slopping procedure and received the Protected Designation of Origin
(PDO) label. To better understand the link between the production area, the microbiome composition and the
flavor profile of the products, we performed a multiomic characterization of PDO BM collected from 57 different
dairies located in the two main PDO production area, i.e. Caserta (n = 35) and Salerno (n = 22). Thus, we
assessed the microbiome by high-throughput shotgun metagenomic sequencing and the Volatile Organic Compounds
(VOCs) by gas chromatography/mass spectrometry (GC/MS). Streptococcus thermophilus, Lactobacillus
helveticus, and Lactobacillus delbrueckii subsp. delbrueckii were identified as the core microbiome present in all
samples. However, the microbiome taxonomic profiles resulted in a clustering of the samples based on their
geographical origin, also showing that BM from Caserta had a greater microbial diversity. Consistently, Caserta
and Salerno samples also showed different VOC profiles. These results suggest that the microbiome and its
specific metabolic activity are part of the terroir that shape BM specific features, linking this traditional product
with the area of production, thus opening new clues for improving traceability and fraud protection of traditional
products
From biodegradable polymers to bioresorbable vascular scaffolds: available evidence in the current era of new-generation DES
No full textNew-generation drug-eluting stents (DES) encompass a large variety of coronary devices, featuring thin struts, biocompatible durable or biodegradable polymer coatings, and limus-eluting drugs. Due to improved early and long-term outcomes among patients undergoing percutaneous coronary intervention, new-generation metallic DES are recommended in almost all patient and lesion subsets. Available evidence from randomized trials indicate a similar safety and efficacy profile between biodegradable and durable polymers new-generation DES. Recently, polymer- free DES provided promising results particularly as alternative to bare-metal stents. Ultimately, although remaining conceptually solid, bioresorbable vascular scaffolds represent an immature technology owing to increased risk of thrombosis. In this review, we summarized current evidence about contemporary coronary devices
Fermented foods, their microbiome and its potential in boosting human health
No full textAbstract Fermented foods (FFs) are part of the cultural heritage of several populations, and their production dates back 8000 years. Over the last ~150 years, the microbial consortia of many of the most widespread FFs have been characterised, leading in some instances to the standardisation of their production. Nevertheless, limited knowledge exists about the microbial communities of local and traditional FFs and their possible effects on human health. Recent findings suggest they might be a valuable source of novel probiotic strains, enriched in nutrients and highly sustainable for the environment. Despite the increasing number of observational studies and randomised controlled trials, it still remains unclear whether and how regular FF consumption is linked with health outcomes and enrichment of the gut microbiome in health‐associated species. This review aims to sum up the knowledge about traditional FFs and their associated microbiomes, outlining the role of fermentation with respect to boosting nutritional profiles and attempting to establish a link between FF consumption and health‐beneficial outcomes
Explainable artificial intelligence and microbiome data for food geographical origin: the Mozzarella di Bufala Campana PDO Case of Study
No full textIdentifying the origin of a food product holds paramount importance in ensuring food safety, quality, and authenticity. Knowing where a food item comes from provides crucial information about its production methods, handling practices, and potential exposure to contaminants. Machine learning techniques play a pivotal role in this process by enabling the analysis of complex data sets to uncover patterns and associations that can reveal the geographical source of a food item. This study aims to investigate the potential use of explainable artificial intelligence for identifying the food origin. The case of study of Mozzarella di Bufala Campana PDO has been considered by examining the composition of the microbiota in each samples. Three different supervised machine learning algorithms have been compared and the best classifier model is represented by Random Forest with an Area Under the Curve (AUC) value of 0.93 and the top accuracy of 0.87. Machine learning models effectively classify origin, offering innovative ways to authenticate regional products and support local economies. Further research can explore microbiota analysis and extend applicability to diverse food products and contexts for enhanced accuracy and broader impact
Defining the role of HIF-2alpha in the pathogenesis of acute myeloid leukemia and exploiting its inhibition as a new therapeutic approach to promote leukemia differentiation and exhaustion
No full textThis dataset represents findings obtained within a project supported by the Italian Ministry of Health (project code: RF-2019-12369841) and focused on investigating the mechanism of myeloid differentiation blockade by the transcription factor HIF-2alpha in acute myeloid leukemia (AML) and the consequences of its inhibition with novel targeted therapies.
We found that in AML HIF-2alpha regulates a specific set of target genes involved in transcriptional repression, leading to inhibition of entire gene clusters linked to myeloid difefrentiation. As a consequence, HIF-2alpha blockade via genetic or pharmacologic approaches leads to myeloid differentiation and leukemia debulking. Also, we positioned HIF-2alpha under direct transcriptional control by the prodifferentiation agent all-trans retinoic acid (ATRA) and demonstrated that HIF-2alpha blockade cooperates with ATRA to trigger AML cell differentiation.
Links related to this dataset contain: 1. A publication describing part of the results funded by this grant; 2. Source data linked to this publication and deposited in the BioStudies repository (accession number: S-SCDT-10_15252-EMMM_202317810); 3. Sequencing data deposited in the GEO repository
Influenza del campo mareale sull'attivita' di vulcani attivi dell'Italia meridionale
No full textDottorato di ricerca in geofisica e vulcanologia. 8. ciclo. Tutore G. CorradoConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal
Seismic response evaluation of non-structural drywall building components: Planning of an experimental campaign
No full textPast earthquakes have shown that the damage to non-structural elements can severely limit the functionality of most affected buildings and cause substantial economic losses. Among the non-structural building components, the ceiling-partition systems represent a large economic investment in construction sector. Nevertheless, their seismic performance is poorly understood, because information and specific guidance are very limited. In the last years, international studies have been conducted on the seismic behaviour of light gage steel stud partition walls (Lee et al. [1], Restrepo and Bersofsky [2], Tasligedik et al. [3], Retamales et al. [4], Magliulo at al. [5]), suspended ceilings (Badillo et al. [6], Magliulo et al. [7], Gilani et al. [8], Soroushian et al. [9],) and partition walls - ceiling systems (Filiatrault et al. [10], McCormick at al. [11]). Since the behaviour of these systems cannot be easily simulated with traditional structural analysis, experimentation is the main way to assess the seismic response. For these reasons, an experimental campaign has been planned at the University of Naples Federico II with the main aim to characterize the seismic response of different Cold-Formed Steel (CFS) partition drywalls and suspended continuous plasterboard ceilings. The current paper presents the research project, in terms of experimental program, prototypes and specifically designed set-up
EZN-2208 treatment suppresses chronic lymphocytic leukaemia by interfering with environmental protection and increases response to fludarabine
No full textThe transcription factor HIF-1α is overexpressed in chronic lymphocytic leukaemia (CLL), where it promotes leukaemia progression by favouring the interaction of leukaemic cells with protective tissue microenvironments. Here, we tested the hypothesis that a pharmacological compound previously shown to inhibit HIF-1α may act as a chemosensitizer by interrupting protective microenvironmental interactions and exposing CLL cells to fludarabine-induced cytotoxicity. We found that the camptothecin-11 analogue EZN-2208 sensitizes CLL cells to fludarabine-induced apoptosis in cytoprotective in vitro cultures; in vivo EZN-2208 improves fludarabine responses, especially in early phases of leukaemia expansion, and exerts significant anti-leukaemia activity, thus suggesting that this or similar compounds may be considered as effective CLL therapeutic approaches
Bacterioplankton diversity and distribution in relation to phytoplankton community structure in the Ross Sea surface waters
No full text© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Cordone, A., D’Errico, G., Magliulo, M., Bolinesi, F., Selci, M., Basili, M., de Marco, R., Saggiomo, M., Rivaro, P., Giovannelli, D., & Mangoni, O. Bacterioplankton diversity and distribution in relation to phytoplankton community structure in the Ross Sea surface waters. Frontiers in Microbiology, 13, (2022): 722900, https://doi.org/10.3389/fmicb.2022.722900.Primary productivity in the Ross Sea region is characterized by intense phytoplankton blooms whose temporal and spatial distribution are driven by changes in environmental conditions as well as interactions with the bacterioplankton community. However, the number of studies reporting the simultaneous diversity of the phytoplankton and bacterioplankton in Antarctic waters are limited. Here, we report data on the bacterial diversity in relation to phytoplankton community structure in the surface waters of the Ross Sea during the Austral summer 2017. Our results show partially overlapping bacterioplankton communities between the stations located in the Terra Nova Bay (TNB) coastal waters and the Ross Sea Open Waters (RSOWs), with a dominance of members belonging to the bacterial phyla Bacteroidetes and Proteobacteria. In the TNB coastal area, microbial communities were characterized by a higher abundance of sequences related to heterotrophic bacterial genera such as Polaribacter spp., together with higher phytoplankton biomass and higher relative abundance of diatoms. On the contrary, the phytoplankton biomass in the RSOW were lower, with relatively higher contribution of haptophytes and a higher abundance of sequences related to oligotrophic and mixothrophic bacterial groups like the Oligotrophic Marine Gammaproteobacteria (OMG) group and SAR11. We show that the rate of diversity change between the two locations is influenced by both abiotic (salinity and the nitrogen to phosphorus ratio) and biotic (phytoplankton community structure) factors. Our data provide new insight into the coexistence of the bacterioplankton and phytoplankton in Antarctic waters, suggesting that specific rather than random interaction contribute to the organic matter cycling in the Southern Ocean.Samples were collected in the framework of Plankton biodiversity and functioning of the Ross Sea ecosystems in a changing Southern Ocean [P-ROSE – (PNRA16_00239)], and CDW Effects on glacial mElting and on Bulk of Fe in the Western Ross sea [CELEBeR – (PNRA16_00207)] projects – Italian National Antarctic Program – funded by the Ministry of Education, University and Research (MIUR), awarded to OM and PR, respectively. MM was supported by an Earth-Life Science Institute (Tokyo, Japan) visiting fellowship. This work was partially supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 948972) to DG
