1,720,999 research outputs found
3P284 Single Particle Analysis Based on Zemike Phase Contrast TEM(Bioimaging,Poster Presentations)
3P025 Single Particle Analysis of Image Data Acquired by Zernike Phase Contrast Transmission Electron Microscope
2P540 Single Particle Analysis of Image Data Acquired by Zernike Phase Contrast Transmission Electron Microscope(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Nagayama K. Transmission electron microscopy with Zernike phase plate. Ultramicroscopy. 2001; 88:243–52. [PubMed: 11545320
Abstract The possibility of implementing a Zernike phase plate in a transmission electron microscope is investigated both theoretically and experimentally. The phase-retarding plate in the form of thin film with a hole in the center is positioned in the back-focal plane of the objective lens. The experiments show that the phase plate functions as predicted, producing a cosine-type phase contrast transfer function. Images of negatively stained horse spleen ferritin were highly improved in the contrast and the image-modulation, compared to those acquired without the phase plate. Charging and related difficulties were encountered during the phase plate experiments. In order to make the technique user-friendly a number of improvements have to be made, and are discussed in terms of the current level of technology and instrumentation.
Complex Observation in Electron Microscopy. II. Direct Visualization of Phases and Amplitudes of Exit Wave Functions
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