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Protective effect of paraoxonase activity in high-density lipoproteins against erythrocyte membranes peroxidation: a comparison between healthy subjects and type 1 diabetic patients
No full textJ Clin Endocrinol Metab. 2004 Jun;89(6):2957-62.
Protective effect of paraoxonase activity in high-density lipoproteins against erythrocyte membranes peroxidation: a comparison between healthy subjects and type 1 diabetic patients.
Ferretti G, Bacchetti T, Busni D, Rabini RA, Curatola G.
SourceInstituto di Biochimica, Università Politecnica delle Marche, I-60131 Ancona, Italy.
Abstract
High-density lipoproteins (HDL) plays a key role in the protection against oxidative damage of lipoprotein and biological membranes. The aim of the present study was to investigate the relationship between the antioxidant role of HDL and the HDL-paraoxonase (PON) activity in healthy subjects and in type 1 diabetic patients. Moreover, the ability of HDL of controls and diabetic patients to protect and/or repair biological membranes from oxidative damage was studied. HDL were isolated from 31 type 1 diabetic patients and 31 sex- and age-matched healthy subjects and immediately used to evaluate lipid hydroperoxides and HDL-PON activity. Erythrocyte membranes obtained from healthy subjects were oxidized with 2,2-azo-bis(2-aminidinopropane)dihydrochloride and then incubated in the presence of HDL isolated from healthy or type 1 diabetic subjects, with measurements of membrane lipid hydroperoxides before and after the incubation. HDL from type 1 diabetic patients showed higher levels of lipid hydroperoxides and a lower activity of HDL-PON than healthy subjects. Moreover, HDL of type 1 diabetic patients protected less efficiently erythrocyte membranes against oxidative damage compared with HDL from healthy subjects. A negative correlation was found between HDL-PON activity and the levels of hydroperoxides of HDL, confirming the relationship between PON and lipid peroxidation and suggesting that subjects with low PON activity are more exposed to oxidative damage than subjects with high PON activity. The ability of HDL to protect erythrocyte membranes was positively correlated with HDL-PON activity and negatively correlated with the levels of lipid hydroperoxides of HDL of healthy subjects. These results confirm a linkage between PON activity and lipid peroxidation of lipoproteins and suggest that the ability of HDL to protect erythrocyte membranes might be related to the PON activity. It might be hypothesized that the decrease of PON activity in diabetic patients and the lower HDL protective action against membrane peroxidation could contribute to acceleration of arteriosclerosis in type 1 diabetes mellitus.
PMID:15181084[PubMed - indexed for MEDLINE
Abnormal membrane fluidity and acetylcholinesterase activity in erythrocytes from insulin-dependent diabetic patients.
No full textModifications induced by diabetes on the physicochemical and functional properties of erythrocyte plasma membrane.
No full textFunctional Reach Test: Movement Strategies in Diabetic Subjects
Full text linkFunctional reach (FR) is a clinical measure, defined as the maximum distance one can reach, forward
beyond arm’s length, able to identify elderly subjects at risk of recurrent falls. Subjects, exhibiting the
same FR can perform the motor task in different ways: a kinematic analysis of the FR, task can help to
identify the motor strategy adopted. The FR test was applied to 17 diabetic non-neuropathic, (CTRL) and
37 neuropathic (DN) subjects. Motor strategies adopted were defined as: ‘‘hip’’ or ‘‘other’’ strategy; the
latter included: ‘‘mixed’’ and ‘‘trunk rotation’’ strategies. Principal Component Analysis and nonparametric
statistical tests were used to study the different execution modalities of the FR test. Results
show that, in CTRL, the most important parameters are those related to trunk flexion in the sagittal plane.
Instead, for DN, the main features are related not only to trunk flexion but also to trunk rotation in the
transverse plane. Percentages of subjects who used ‘‘hip’’ or ‘‘other’’ strategies are similar for CTRL and
DN subjects. However, within the ‘‘other’’ strategy group, the percentage of DN that used a ‘‘trunk
rotation’’ strategy was much higher than for CTRL. Results show that individuals, although exhibiting the
same reaching distance, adopt different movement strategies. Consequently it is important to evaluate
the kinematic behaviour and not only the clinical measure, because the evaluation of the motor strategy
might be useful in the early detection of subjects at risk of postural instabilit
Alteration in fatty acid composition and susceptibility to peroxidation of very low density lipoproteins during diabetes mellitus
No full text
Aumentata suscettibilità alla perossidazione delle VLDL e HDL di pazienti diabetici non insulino-dipendenti: una possibile correlazione con la composizione in acidi grassi
No full textENTROPIA APPROSSIMATA SU DATI POSTUROGRAFICI DI SOGGETTI DIABETICI CON NEUROPATIA PERIFERICA
No full textLow density lipoproteins from type 1 diabetic patients modify platelet functions: a possible role of lysophosphatidylcholine.
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