1,721,225 research outputs found
Un approccio integrato e relazionale al metabolismo urbano e alla sua spazialità
No full textSempre più frequentemente ci troviamo a confrontarci con l’introduzione e la diffusione di categorie interpretative innovative che semplicemente danno nome alla presenza di fenomeni complessi che attraversano la città e l’ambiente urbano e che l’urbanistica e la pianificazione, nella loro declinazione radicata nella cultura della modernità, non hanno saputo per tanto tempo affrontare. O, meglio, ormai da molto tempo se ne conosce l’esistenza (pensiamo, ad esempio, a tutta la questione ambientale), ma si è fatto sempre tendenzialmente ricorso ad approcci consolidati, tipici della pianificazione fisica tradizionale, che mal si adattano a questi problemi, in particolare ad esempio a quelli ambientali.
Molte sono le retoriche che si annidano nell’uso di categorie interpretative innovative e queste bisogna avere ben presenti se vogliamo che effettivamente siano incisive e ci parlino di una reale innovazione nel modo di pensare l’urbanistica. In particolare, bisogna rivolgere una specifica attenzione a quali siano i soggetti sociali protagonisti di questa innovazione e dei processi che vengono attivati. Il protagonismo sociale, che sta effettivamente realizzando oggi questi approcci nel concreto della città, costituisce il vero potenziale di cambiamento e innovazione.More and more frequently, we find ourselves dealing with the introduction and diffusion of innovative interpretative categories that simply give name to the presence of complex phenomena that cross the city and the urban environment and that planning has not been able to deal with for so long. Or, better, for a long time now we know its existence (let's think, for example, of the whole environmental question), but we have always tended to resort to consolidated approaches, typical of traditional physical planning, which are ill-suited to these problems, in particular for example environmental problems.
There are many rhetorics in the use of innovative interpretative categories and these must be well present if we want them to be effective and speak to us of a real innovation in the way of thinking about urban planning. In particular, specific attention must be given to which social subjects are the protagonists of this innovation and of the processes that are activated. The social protagonism, which is actually realizing these approaches today in the concrete of the city, constitutes the true potential for change and innovation
Probiotic properties of bifidobacteria
No full textBifidobacteria are major components of the indigenous bacterial population present in the human gut and are arguably most relevant to the health-promoting properties that have been attributed to elements of this microbiota. They exert a range of beneficial health effects, including the regulation of intestinal microbial homeostasis, the inhibition of pathogens and harmful bacteria that colonize and/or infect the gut mucosa, the modulation of local and systemic immune responses, the repression of procarcinogenic enzymatic activities within the microbiota, the production of vitamins, and the bioconversion of a number of dietary compounds into bioactive molecules. This chapter summarizes the reported health-promoting properties of members of the genus Bifidobacterium and discusses what research is still necessary for an in depth understanding of the probiotic function. In fact, although experimental evidence of the probiotic effectiveness of bifidobacteria has a long history, little information is available on the molecular mechanisms underlying the health-promoting claims, especially on such complex phenomena as anticarcinogenic and anti-inflammatory effects
Synthesis and antibacterial activity of new 3-diazoindole-2-carboxamides
No full textSome 3-diazoindole-2-carboxamides have been synthesized and their antimicrobial activity have been tested. Antimicrobial activity was practically lacking
An efficient transformation system for Bifidobacterium spp.
No full textThis study describes a broad host transformation protocol that enables the uptake of plasmid DNA into 10 different species of Bifidobacterium, some of which have never been transformed before. The vector pNC7 (4·9 kb) was used to optimize the electroporation protocol. Transformation efficiencies ranged from 3·6×10−1 to 1·2×105 transformations per μg DNA. The impact of growth medium composition and electric field strength on transformation efficiency were independently optimized. Electrocompetent cells were grown in Iwata medium broth enriched with ActilightRP 16%, harvested during the early exponential growth phase, and pulsed at 12·5 kV cm−1, 100 Ω and 25 μF
Improved cloning vectors for Bifidobacterium spp.
No full textThe recombinant plasmids pDLI41, pDGA7 and pDCO7 were constructed by cloning in pDG7, a vector based on Bifidobacterium longum replicon pMB1, the following heterologous genes: Pseudomonas fluorescens lipase, Bacillus licheniformis alpha-amylase and Streptomyces sp. cholesterol oxidase. The hybrid plasmids efficiently transformed Bifidobacterium belonging to five different species. A novel Escherichia coli-Bifidobacterium set of shuttle vectors based on the replicon pMB1 (pLF5, pCLJ15, pSPEC1) featuring chloramphenicol, erythromycin and spectinomycin resistance genetic determinants as selection marker for bifidobacteria, was developed. The plasmid pTRE3, a derivative of pLF5, was the smallest (2.8 kb) Bifidobacterium vector, possessed a convenient multicloning site and presented high structural and segregational stability
Some new 3-methoxy-5-methyl-1,4-substituted pyrazoles
No full textA series of 3-methoxypyrazole derivatives was synthesized and tested as antifungal agents. The substituents were chosen on the base of their lipophylicity and for their presence in well-known antifungal drugs. The compounds displayed no significant activity in vitro
Allylamine type xanthone antimycotics
No full textA number of xanthone derivatives bearing the basic chain of naftifine and butenafine antimycotics in 1, 2, 3, and 4 nuclear positions are described. The in vitro antifungal activity against representative strains of molds and yeasts is reported. Only butenafine xanthone analogues show significant activity against Cryptococcus neoformans, in particular the regioisomer 4d (1.5 micrograms/ml)
The effect of n-alkanes in the degradation of dibenzothiophene and of organic sulfur compounds in heavy oil by a Pseudomonas sp.
No full textThe microbial degradation of organic sulfur compounds was examined in aerobic conditions employing a pure culture of aPseudomonas sp., isolated from the soil. The effect ofn-alkanes on the degradation of dibenzothiophene (DBT) showed that the assimilation of the sulfur compound by the microorganism is favoured byn-dodecane. Moreover, the saturated fraction was seen to enhance the degradation of the sulfur compounds to be found in a deasphaltenated heavy oil
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