5,011 research outputs found
Author Interview with Brian D. Anderson
No full textBrian D. Anderson was our feature artist of the week, October 19th - 23rd, 2020.https://jagworks.southalabama.edu/vid_presentations/1010/thumbnail.jp
Art Behind Gaming: Brian D. Anderson
No full textA discussion with author Brian D. Anderson about worldbuilding in fantasy. Part of the Art Behind Gaming Online Con.https://jagworks.southalabama.edu/vid_presentations/1046/thumbnail.jp
Limited proteolysis and site-directed mutagenesis reveal the origin of microheterogeneity in Rhodotorula gracilis D-amino acid oxidase
No full textWhen analysed by isoelectric focusing, D-amino acid oxidase from the yeast Rhodotorula gracilis normally consists of three molecular isoforms (pI 7.8, 7.4 and 7.2, respectively) all with the same N-terminal sequence. However, only a single band of pI 7.8 is detected with the recombinant wild-type protein expressed in E. coli. To determine whether the molecular basis of this heterogeneity is due to proteolysed forms of the protein, we treated R. gracilis D-amino acid oxidase with various proteases. Limited proteolysis by chymotrypsin and thermolysin produced truncated and nicked monomeric holoenzymes containing two polypeptides of ≃ 34 kDa (Met1-Leu312) and one of ≃ 5 kDa (Ala319-Arg364 with chymotrypsin or Ala319-Ala362 with thermolysin). On the other hand, treatment with endoproteinase Glu-C gave a dimeric holoenzyme lacking the C-terminal SKL tripeptide. This cleavage of Glu365-Ser366 peptide bond caused the disappearance of the three isoelectric bands and a single homogeneous band (pI 7.2) appeared. To study this protein form, we used site-directed mutagenesis to produce a mutant form of R. gracilis D-amino acid oxidase lacking the SKL C-terminal tripeptide (which is the targeting sequence PTS1 for peroxisomal proteins). As expected, the SKL-deleted mutant gave a single band (pI 7.2) in isoelectric focusing. The three-band pattern of native yeast enzyme was generated by in vitro experiments using an equimolar mixture of the wild-type (pI 7.8) and the SKL-deleted recombinant (pI 7.2) DAAOs. The microheterogeneity of yeast DAAO thus stems from the association of two polypeptide chains differing in the C-terminal tripeptide, giving three different holoenzyme dimers
Competition policy. by Brian Ellis
No full texttag=1 data=Competition policy. by Brian Ellis
tag=2 data=Ellis, Brian
tag=3 data=Australian Rationalist,
tag=5 data=46
tag=6 data=Autumn/Winter 1998
tag=7 data=51-56.
tag=8 data=ECONOMIC CONDITIONS
tag=9 data=COMPETITION%CORPORATISATION%NATIONAL COMPETITION POLICY%PRIVATE SECTOR PUBLIC SECTOR EFFECTIVENESS%SERVICE DELIVERY%SOCIAL POLICY%INNOVATION
tag=10 data=Examines the Government's National Competition Policy in relation to encouraging R&D, and the corporisation of public services and utilites. The author is Emeritus Professor of Philosophy at La Trobe UNiversity and Vice-President of the Rationalist Society of Australia. Article Taken from What's New.
tag=13 data=CABExamines the Government's National Competition Policy in relation to encouraging R&D, and the corporisation of public services and utilites. The author is Emeritus Professor of Philosophy at La Trobe UNiversity and Vice-President of the Rationalist Society of Australia. Article Taken from What's New
Ross, Brian D.: A Surgeon “Discovers” NMR - the Development of Clinical Neurospectroscopy: 1980-2010
No full textPhase-Function Normalization in the 3-D Discrete-Ordinates Solution of Radiative Transfer – PART I: Conservation of Scattered Energy and Asymmetry Factor
No full textThe conditions for which conversation of scattered energy and phase-function asymmetry factor after discrete-ordinates methods (DOM) directional discretization for 3-D radiative transfer in anisotropic scattering media breaks down are examined. Directional discretization in anisotropic scattering media is found to alter the scattering asymmetry factor—a second-type of ‘‘false scattering.’’ Phase-function normalization which conserves scattered energy alone cannot correct this problem, and conservation of the asymmetry factor is simultaneously required. A normalization technique developed by the authors, which was successfully tested in 2-D asymmetric cylindrical-coordinate radiative transfer analysis, is intensively examined and validated with benchmark problems in 3-D Cartesian coordinates. In Part I of this study, the degree of anisotropy for which normalization is necessary to conserve these inherent quantities is presented for various phase-function approximations and discrete quadrature sets.Peer reviewed
Phase-Function Normalization in the 3-D Discrete-Ordinates Solution of Radiative Transfer – PART II: Benchmark Comparisons
No full textRadiative transfer in a cubic enclosure, subject to varying conditions, is determined using the discrete-ordinates method (DOM) with the two normalization techniques introduced in Part I of this study. Their predictions are compared with Monte Carlo simulations. For all cases, false scattering due to directional discretization cannot be corrected when the old technique, which solely conserves scattered energy, is implemented; and thus, signifi- cant discrepancies exist when compared to Monte Carlo results. The new technique, which conserves both scattered energy and the asymmetry factor, is able to retain original scatter- ing properties after directional discretization, leading to improved accuracy when compared to Monte Carlo. In addition, a parametric study is presented to gauge the impact of asym- metry-factor conservation on media with various optical properties. Finally, the impact of normalization is investigated for both ultrafast radiative transfer and ballistic incidence with varying incident angle.Peer reviewed
Improved treatment of anisotropic scattering in radiation transfer analysis using the finite volume method
No full textDiscretization of the integral anisotropic-scattering term in the equation of radiative transfer will result in two kinds of numerical errors: alterations in scattered energy and asymmetry factor. Though quadrature flexibility with large angular directions and further solid-angle splitting in the finite volume method (FVM) allow for reduction/minimization of these errors, computational efficiency is adversely impacted. A phase-function normalization technique to get rid of these errors is simpler and is applied to the three-dimensional (3-D) FVM for the first time to improve anisotropic radiation transfer computation accuracy and efficiency. FVM results are compared to Monte Carlo and discrete-ordinates method predictions of radiative heat transfer in a cubic enclosure housing a highly anisotropic participating medium. It is found that the FVM results generated using the normalization technique conform accurately to the results of the other two methods with little impact on computational efficiency.Peer reviewed
Comparison of Quadrature Schemes in DOM for Anisotropic Scattering Radiative Transfer Analysis
No full textThe commonly implemented level-symmetric SN quadrature set for the discrete-ordinates method suffers from a limitation in discrete direction number to avoid physically unrealistic weighting factors. This limitation can have an adverse impact for determining radiative transfer, as directional discretization results in angular false scattering errors due to distortion of the scattering phase function in addition to the ray effect. To combat this limitation, several higher-order quadrature schemes with no directional limitation have been developed. Here, four higher-order quadrature sets (Legendre-equal weight, Legendre-Chebyshev, triangle tessellation, and spherical ring approximation) are implemented for determination of radiative transfer in a 3-D cubic enclosure containing participating media. Heat fluxes obtained at low direction number are compared to the SN quadrature and Monte Carlo predictions to gauge and compare quadrature accuracy. Investigation into the reduction/elimination of angular false scattering with increase in direction number, including heat flux accuracy with respect to Monte Carlo and computational efficiency, is presented. It is found that while the higher-order quadrature sets are able to effectively minimize angular false scattering, the number of directions required is extremely large, and thus it is more computationally efficient to implement proper phase-function normalization to obtain accurate results.Peer reviewed
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