1,721,082 research outputs found
The Use of PowerPlex® Y23 System with the ABI PRISM® 310 Genetic Analyzer
No full textOver the last fifteen years, multiplex PCR typing of Y-chromosomal short tandem repeat (STR) loci has emerged as a powerful tool for forensic casework analysis, especially in sexual assault cases, where evidence typically consists of mixtures containing large amounts of female DNA in the presence of minor amounts of male DNA. However, since Y-STR loci are from nonrecombining portion of the Y chromosome, individuals from the same lineage typically share the same profile. Therefore, Y-STR profiles do not carry the same discrimination power as autosomal profiles. The availability of new multiplex PCR assays that allow simultaneous typing of conventional and additional Y-STRs improves the power of discrimination of paternal lineages and is therefore highly welcomed by the forensic community. Recently Promega launched the PowerPlex® Y23 System, which includes the Y-STR loci found in the AmpFlSTR® Yfiler® PCR Amplification Kit from Life Technologies plus six new loci that display high genetic diversity in human populations. Two of the loci, DYS570 and DYS576, are rapidly mutating loci (1) .
The PowerPlex® Y23 System Technical Manual TMD035 describes instrument setup and sample preparation of PCR products using the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Here, we report the successful analysis of amplicons obtained with PowerPlex® Y23 on the ABI PRISM® 310 Genetic Analyzer. We performed sensitivity and mixture studies using control DNA samples and compared typing results in a limited set of casework samples previously typed with the Yfiler® kit
Applicazione di test immunocromatografici per l’identificazione della naura di tracce biologiche
No full textDevelopment of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample
No full textTwo multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101–DXS7424 and DXS6789–DXS6801–DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man
Subtyping of Y-chromosomal haplogroup E-M78 (E1b1b1a) by SNP assay and its forensic application.
No full textThe continual discovery of new single-nucleotide polymorphisms (SNPs) has led to an increased resolution of the Y chromosome phylogeny. Some of these Y-SNPs have shown to be restricted to small geographical regions and therefore may prove useful in the forensic field as tools for the prediction of population of origin of unknown casework samples. Here, we describe a system for the molecular dissection of haplogroup E-M78 (E1b1b1a), consisting of multiplex polymerase chain reaction and minisequencing of M78 and nine population-informative Y-SNPs (M148, M224, V12, V13, V19, V22, V27, V32, V65) in a single reaction. Sensitivity and admixture studies demonstrated that the SNP protocol allows robust genotyping from as little as 50 pg of male DNA, even in the presence of 500-fold amounts of female DNA. In order to evaluate the suitability of E1b1b1a, subhaplogrouping for population-of-origin prediction, the distribution of E-M78 and its derived variants was determined in an Italian population sample (n = 326)
Applicazione di test immunocromatografici per l'identificazione della natura di tracce biologiche
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