103,594 research outputs found
Rinaudo Jean, Coste Robert — Initiation aux sciences économiques
No full textT. G. Rinaudo Jean, Coste Robert — Initiation aux sciences économiques. In: Population, 24ᵉ année, n°3, 1969. pp. 583-584
GIS And Web-GIS, Commercial and Open Source Platforms: General Rules for Cultural Heritage Documentation
No full textSR-4000 and CamCube3.0 Time of Flight (ToF) Cameras: Tests and Comparison
No full textIn this paper experimental comparisons between two Time-of-Flight (ToF) cameras are reported in order to test their performance and to give some procedures for testing data delivered by this kind of technology. In particular, the SR-4000 camera by Mesa Imaging AG and the CamCube3.0 by PMD Technologies have been evaluated since they have good performances and are well known to researchers dealing with Time-of- Flight (ToF) cameras. After a brief overview of commercial ToF cameras available on the market and the main specifications of the tested devices, two topics are presented in this paper. First, the influence of camera warm-up on distance measurement is analyzed: a warm-up of 40 minutes is suggested to obtain the measurement stability, especially in the case of the CamCube3.0 camera, that exhibits distance measurement variations of several centimeters. Secondly, the variation of distance measurement precision variation over integration time is presented: distance measurement precisions of some millimeters are obtained in both cases. Finally, a comparison between the two cameras based on the experiments and some information about future work on evaluation of sunlight influence on distance measurements are reporte
Archaeological site monitoring: UAV photogrammetry can be an answer
No full textDuring archaeological excavations it is important to monitor the new excavated areas and findings day by day in order to be able to plan future excavation activities. At present, this daily activity is usually performed by using total stations, which survey the changes of the archaeological site: the surveyors are asked to produce day by day draft plans and sections which allow archaeologists to plan their future activities. The survey is realized during the excavations or just at the end of every working day and drawings have to be produced as soon as possible in order to allow the comprehension of the work done and to plan the activities for the following day. By using this technique, all the measurements, even those not necessary for the day after, have to be acquired in order to avoid a ‘loss of memory'. A possible alternative to this traditional approach is aerial photogrammetry, if the images can be acquired quickly and at a taken distance able to guarantee the necessary accuracy of a few centimeters. Today the use of UAVs (Unmanned Aerial Vehicles) can be considered a proven technology able to acquire images at distances ranging from 4 m up to 20 m: and therefore as a possible monitoring system to provide the necessary information to the archaeologists day by day. The control network, usually present at each archaeological site, can give the stable control points useful for orienting a photogrammetric block acquired by using an UAV equipped with a calibrated digital camera and a navigation control system able to drive the aircraft following a pre-planned flight scheme. Modern digital photogrammetric software can solve for the block orientation and generate a DSM automatically, allowing rapid orthophoto generation and the possibility of producing sections and plans. The present paper describes a low cost UAV system realized by the research group of the Politecnico di Torino and tested on a Roman villa archaeological site located in Aquileia (Italy), a well-known UNESCO WHL site. The results of automatic orientation and orthophoto production are described in terms of their accuracy and the completeness of information guaranteed for archaeological site excavation managemen
STEREOBIT/20 and RESEAU. A new photogrammetric analytical system for semimetric images: image acquisition and practical examples
No full textStructure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus
No full textIn group B Streptococcus (GBS), 3 structurally distinct types of pili have been discovered as potential virulence factors and vaccine candidates. The pilus-forming proteins are assembled into high-molecular-weight polymers via a transpeptidation mechanism mediated by specific class C sortases. Using a multidisciplinary approach including bioinformatics, structural and biochemical studies, and in vivo mutagenesis, we performed a broad characterization of GBS sortase C1 of pilus island 2a. The high-resolution X-ray structure of the enzyme revealed that the active site, into the β-barrel core of the enzyme, is made of the catalytic triad His157-Cys219-Arg228 and covered by a loop, known as the “lid.” We show that the catalytic triad and the predicted N- and C-terminal transmembrane regions are required for the enzyme activity. Interestingly, by in vivo complementation mutagenesis studies, we found that the deletion of the entire lid loop or mutations in specific lid key residues had no effect on catalytic activity of the enzyme. In addition, kinetic characterizations of recombinant enzymes indicate that the lid mutants can still recognize and cleave the substrate-mimicking peptide at least as well as the wild-type protein.—Cozzi, R., Malito, E., Nuccitelli, A., D’Onofrio, M., Martinelli, M., Ferlenghi, I., Grandi, G., Telford, J. L., Maione, D., Rinaudo, C. D. Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus
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