1,721,100 research outputs found
Assessment of peloids used for the Spa therapy.
No full textNella Medicina Termale i fanghi o peloidi
sono sicuramente tra i presidi terapeutici più
utilizzati. Gli effetti della loro applicazione
sono suddivisi in locali (nella sede d'applicazione)
e generali (a livello dell' intero organismo).
Gli effetti locali originano da stimoli indotti
dalla fangoterapia su singoli distretti
corporei, ma determinano una risposta di
tutto l'organismo ed il coinvolgimento di
numerosi organi, apparati e sistemi metabolici.
A loro volta le reazioni generali indotte
dalla fango terapia possono influire sulla
risposta locale. Vi è quindi un rapporto biunivoco
tra i due meccanismi ed una serie di
azioni e reazioni.
Un' altra possibile suddivisione è quella che
distingue gli effetti specifici e aspecifici della
fangoterapia. I primi sono legati per lo più al
particolare tipo di acqua minerale contenuta
nel fango (azione attribuita ai singoli mineralizzatori
ed ai gas disciolti nell' acqua) e alla
$
microflora che in esso si sviluppa durante
la maturazione. I secondi effetti, aspecifici,
sono da ricondursi sia all'azione biologica e
terapeutica del calore (termoterapia) che alle
altre proprietà fisiche proprie della metodica
e del mezzo impiegato. Questi effetti specifici
ed aspecifici possono a loro volta esplicarsi a
livello locale e generale. Il risultato terapeutico
complessivo della fangoterapia è comunque
influenzato in parte dalla reattività individuale
del paziente ed in parte dipende dal tipo di
fango e dalle modalità di applicazione.
Gli effetti biologici e terapeutici sono fondamentalmente
riconducibili a:
- azione antinfiammatoria;
azione analgesica;
- azione miorilassante;
- azione eutrofica;
- aumento delle resistenze verso le noxae
patogene esogene ed endogene;
- azione di stimolo in numerosi processi
metabolici
Troubleshooting and improving the mouse and rat isolated perfused liver preparation.
Full text linkIsolated Perfused Liver (IPL) model is widely performed in rats but mouse liver is used also, although a detailed description of this procedure is absent. A comparison of the different techniques used on rats and mice will be discussed in this article, associated with a detailed description of the surgical and technical aspects needed to obtain and maintain the integrity of the livers during the organ isolation and perfusion. The surgery procedures, the IPL set-up, and the evaluation of hepatic function and damage will be described in relation to both rats and mice. In particular, the heparin dosage and administration, the portal vein cannulation avoiding portal leakage, the use of supra hepatic caval vein output, and the insertion of a cannula for bile collection will be reported. For the settings, the perfusion circuit, the perfusion solution, the temperature and the flow rate will be described, with particular regard to the balance between perfusion pressure and oxygen delivery. The monitoring of liver integrity by measuring oxygen concentration and calculating oxygen delivery rate and oxygen uptake rate, and recommendations for the collection of perfusate and bile samples will be considered. Accurate pH measurement with normalization, and the perfusion portal pressure assay by a calibrated water manometer will be also reported. This work analyzes the parameters crucial to performing a correct IPL both in rat and mouse, comparing our experience with the equivalent practice from other laboratories. An updated example of IPL applications in liver toxicology and pharmacology, physiology and pathophysiology, and liver graft preservation will be briefly presented, underlining how this technique provides essential information allowing a more accurate planning of the in vivo studies
The biochemical dissection of the cytoskeleton reveals oxidative stress-induced alteration in intermediate filament-nuclear matrix scaffeld of BT-20 cells
No full textRole of matrix metalloproteinases in cholestasis and hepatic ischemia/reperfusion injury: A review
Full text linkMatrix metalloproteinases (MMPs) are a family of proteases using zinc-dependent catalysis to break down extracellular matrix (ECM) components, allowing cell movement and tissue reorganization. Like many other proteases, MMPs are produced as zymogens, an inactive form, which are activated after their release from cells. Hepatic ischemia/reperfusion (I/R) is associated with MMP activation and release, with profound effects on tissue integrity: their inappropriate, prolonged or excessive expression has harmful consequences for the liver. Kupffer cells and hepatic stellate cells can secrete MMPs though sinusoidal endothelial cells are a further source of MMPs. After liver transplantation, biliary complications are mainly attributable to cholangiocytes, which, compared with hepatocytes, are particularly susceptible to injury and ultimately a major cause of increased graft dysfunction and patient morbidity. This paper focuses on liver I/R injury and cholestasis and reviews factors and mechanisms involved in MMP activation together with synthetic compounds used in their regulation. In this respect, recent data have demonstrated that the role of MMPs during I/R may go beyond the mere destruction of the ECM and may be much more complex than previously thought. We thus discuss the role of MMPs as an important factor in cholestasis associated with I/R injury
Modulation of Neurogenic inflammation in osteoarthrosis patients undergoing a combined treatment of mud packs, thermal baths and acetaminophen: a preliminary study.
No full text- …
