1,721,051 research outputs found
Simultaneous double labelling of routinely processed paraffin tissue sections using combined immunoperoxidase, immunofluorescence, and digital image editing
No full textAn innovative image editing system based on a sequential immunoperoxidase-immunofluorescence technique on routine histological sections is described. With this technique it is possible to identify different antigens in different cells, as well as co-localised antigens in the same cell. The method uses digital image editing to mix two independently captured images into one merged image. The technique was performed with indirect immunoperoxidase, followed by sequential indirect immunofluorescence, digital image acquisition and image editing. Multiple staining examples using anti-cytokeratin, anti-vimentin and anti-calbindin antibodies on canine skin and cerebellum, and feline pleural mesothelioma sections were performed in order to investigate the capabilities of the proposed technique. Our data demonstrated that this method can be easily used to assess multiple protein staining studies with minimum laboratory equipment, and that it allows a better structural visualisation of the tissue morphology compared to double immunofluorescence. Moreover, in contrast to double-immunoperoxidase, with this method it is possible to easily co-localise two different antigens in the same cell compartment. (C) 2009 Elsevier Ltd. All rights reserved
Aberrant sub-cellular immuno-localisation of NOTCH-1 activated intracellular domain (NICD) in feline mammary tumours
Full text linkNOTCH-1 is a trans-membrane receptor protein. Ligand proteins expressed on the surface of neighbouring cells bind to the NOTCH-1 extracellular domain by juxtacrine signaling and release the NOTCH-intracellular-domain (NICD) to alter gene expression. Forty feline mammary lesions (34 malignant, 6 benign/hyperplastic) were submitted for immunohistochemical (IHC) analysis of NICD using an anti-feline NICD monoclonal antibody. Association between NICD expression in the carcinomas and both morphologic parameters and overall survival (OS) were investigated. NICD nuclear expression was observed in benign lesions (100%) while cytoplasmic localisation was evident in carcinomas (0% nuclear positive; 87.5% cytoplasmic positive; 12.5% negative). Cytoplasmic NICD localisation was statistically associated with carcinomas while nuclear staining was associated with hyperplasia. No significant correlation between positive or negative IHC NICD expression and OS or morphological parameters investigated was detected. NOTCH-1 activation, immunohistochemically identified by the NICD active form, seems to play a role in feline mammary carcinoma biology since the majority of tumours express this protein. Nuclear localisation is consistent with the traditional NICD metabolic intra-nuclear pathway while cytoplasmic accumulation suggests an aberrant NOTCH-1 signaling typical of carcinomatous transformation
Immunolocalizzazione differenziale del dominio intracellulare di NOTCH-1 nei tumori mammari del gatto
No full textCanine invasive lobular carcinoma of the mammary gland: morphological and immunohistochemical characterization of three cases
No full textDifferential sub-cellular immuno-localization of NOTCH-1 activated intracellular domain (NICD) in feline mammary tumours
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Correlation between Tumour Associated Macrophage (TAM) Infiltration and Mitotic Activity in Canine Soft Tissue Sarcomas†
Full text linkTumour-associated macrophages (TAMs) are an important part of the tumour microen-vironment but knowledge of their distribution in canine soft tissue sarcomas (STSs) is limited to absent. We analysed 38 STSs retrieved from the veterinary pathology archive; oral and visceral STSs, synovial cell sarcoma, tumours of histiocytic origin, haemangiosarcoma, carcinosarcomas, and undifferentiated tumours were excluded. Iba-1 positive, non-neoplastic tumour infiltrating cells (morphologically indicative of macrophages) were classified as TAMs and were counted in 10 consecutive tumours areas, where no necrosis or other inflammatory cells could be identified. Associations between numbers of TAMs and mitoses, differentiation, and necrosis scores or grade were investigated. TAMs were evident in all STSs and ranged between 6% to 62% of the cells in the microscopic field. The number of TAMs positively correlated with the STSs’ histologic grade. When the components of the grade were analysed separately, TAMs were statistically correlated with mitoses, but not with differentiation or necrosis score. The present findings suggest that TAMs are present in higher numbers when STS proliferation is the predominant feature that drives tumour grade. The abundant presence of TAMs in high-grade STSs may also increase the likelihood of a pathologist misdiagnosing STS for histiocytic sarcoma
Studio morfologico ed immunoistochimico di un carcinoma mammario in una tigre siberiana (Panthera tigris altaica)
No full textExpression of leptin and leptin receptor (OB-R) in normal, hyperplastic, and neoplastic canine mammary tissues
No full textMammary carcinoma in a tiger (Panthera tigris): morphological and immunohistochemical study
No full textThe histologic and immunohistochemical features of a case of mammary gland carcinoma are described in a 14-yr-old female tiger (Panthero tigris). Immunoreactivity to estrogen receptor (ER), progesterone receptor (PR), tumoral protein 53 (p53), vascular endothelial growth factor (VEGF), human epidermal growth factor receptor 2 (HER-2), and cyclooxigenase-2 (COX-2) was investigated. Neoplastic cells were negative for ER, PR, and p53 but showed positivity for VEGF, HER-2, and COX-2, both in the primary and the metastatic lesions. Histopathologic findings and immunohistochemistry results suggested that the malignant behavior of the reported case could be comparable with some aggressive cat mammary carcinomas
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