1,721,001 research outputs found
Characterization and expression of two members of the peach 1-aminocyclopropane-1-carboxylate oxidase gene family.
No full textAnalisi dei promotori dell' 1-amminociclopropano carbossilato ossidasi in Prunus persica L. Batsch.
No full textanalisi di promotori di geni identificati in Prunus persic
Utilizzo di un microarray (microPEACH1.0) per lo studio del trascrittoma durante la maturazione della pesca
No full textORGANIZATION AND STRUCTURE OF 1-AMINOCYCLOPROPANE 1 CARBOXYLATE OXIDASE GENE FAMILY FROM PEACH
No full textCharacterization and expression of two genes encoding ethylene receptors in peach fruit
No full textWe have isolated two peach (Prunus persica) genes, Pp-ETR1 and Pp-ERS1, homolog to the Arabidopsis ethylene receptor ETR1 and ERS1. Pp-ETR1 is identical, in terms of exons number and introns position, to At-ETR1 although the first and fifth intron are 5 and 20 times longer, respectively. In addition two putative polyadenilation sites, that may cause an incomplete splicing at the 3' terminus, are present into the fifth intron. Translation of such a truncated transcript would lead to a product missing a large portion of the receiver domain. The coding region of Pp-ERS1 is organized in five exons interrupted by four introns, but unlike Pp-ETR1 no marked differences in terms of intron length between At-ERS1 and Pp-ERS1 have been detected. Into the promoter region of Pp-ERS1 a motif of 28 nt, which shows high homology with binding ethylene-factors detected in genes up-regulated by ethylene, is present. The deduced protein of both genes contain a sensor domain and the Histidine kinase domain, in which residues, thought to be important for the normal function of ETR1 and ERS-type proteins as ethylene receptors are conserved. These results indicate that Pp-ETR1 and Pp-ERS1 could be functional ethylene receptors with the ability to bind ethylene. Expression analysis, carried out by quantitative RT-PCR, was performed during fruit ripening (cv Maria Marta). The level of Pp-ETR1 transcripts remained unchanged throughout ripening, whereas Pp-ERS1 mRNA increased in parallel with the ethylene climacteric
Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission
No full textTranscriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP
No full textA large-scale transcriptome analysis has been conducted using mPEACH1.0 microarray on nectarine (Prunus persica L. Batsch) fruit treated with 1-methylcyclopropene (1-MCP). 1-MCP maintained flesh firmness but did not block ethylene biosynthesis. Compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. The effect of 1-MCP was confirmed by a direct comparison of transcript profiles in treated and untreated fruit after 24 h of incubation with 106 targets differentially expressed. About 30% of these targets correspond to genes involved in primary metabolism and response processes related to ethylene, auxin, and other hormones. In treated fruit, altered transcript accumulation was detected for some genes with a role in ripening-related events such as softening, colour development, and sugar metabolism. A rapid decrease in flesh firmness and an increase in ethylene production were observed in treated fruit maintained for 48 h in air at 20 oC after the end of the incubation period. Microarray comparison of this sample with untreated fruit 24 h after harvest revealed that about 45% of the genes affected by 1-MCP at the end of the incubation period changed their expression during the following 48 h in air. Among these genes, an ethylene receptor (ETR2) and three ethylene responsive factors (ERF) were present, together with other transcription factors and ethylene-dependent genes involved in quality parameter changes
Suitable reference genes for accurate gene expression analysis in Papaver rhoeas under 2,4-D herbicide stress
No full textPeaches harvested at advanced ripening stage: postharvest for maintaining quality
No full textConsumers of peaches and nectarines often complain of the low eating quality of these fruits that, for commercial purposes, are harvested before their physiological maturation is completed on the tree. The higher quality attained by delaying harvest contradicts the possibility of prolonging shelf-life, particularly for those varieties characterised by a very rapid softening process. In previous works (Tonutti et al., 1998; Bonghi et al., 1999) we have shown that Ultra Low Oxygen (ULO) postharvest treatments (up to 48h) without refrigeration are effective in reducing the softening rate in Springcrest peaches. Considering the differences existing among peach varieties in terms of ripening physiology, the aim of the present work was to evaluate the effects of a short-term ULO treatment on white (WF) and yellow (YF) flesh peach varieties harvested at an advanced ripening stage
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