4 research outputs found
Aislamiento, caracterización y actividad antifúngica de metabolitos secundarios a partir de Piper carpunya Ruiz & Pav
No full textEn el presente trabajo de investigación se determinó la actividad antifúngica del extracto MeOH y de la fracción 21-23 aislada mediante Cromatografía en Columna a partir del extracto DCM de la planta medicinal Piper carpunya Ruiz & Pav. (Guabiduca Dulce). Dicha fracción se la obtuvo al eluir la columna con Hex/AcOEt en la proporción 9:1 v/v. Se aisló también un ácido graso a partir de la fracción 62-65 eluída con Hex/AcOEt en la proporción 75:25 v/v.
Las pruebas antifúngicas realizadas al extracto total y a la fracción fueron determinadas contra dos cepas de hongos dermatofitos Trichophyton mentagrophytes (Tm) y Trichophyton rubrum (Tr) el extracto total mostró un CMI de 500 µg/mL frente a T. Mentagrophytes y T. rubrum y la fracción 21-23 mostró un CMI de 250 µg/mL frente a T. Mentagrophytes y 125 µg/mL frente a T. rubrum
Phytochemical Study of the Ecuadorian Species Lepechinia mutica (Benth.) Epling and High Antifungal Activity of Carnosol against Pyricularia oryzae
No full textThe plant Lepechinia mutica (Benth.) Epling (family Lamiaceae) is endemic to Ecuador. In the present study, we report some major non-volatile secondary metabolites from the leaves and the chemistry of the essential oil distilled from the flowers. The main identified compounds were carnosol, viridiflorol, ursolic acid, oleanolic acid, chrysothol, and 5-hydroxy-4′,7-dimethoxy flavone. Their structures were determined by X-ray diffraction and NMR and MS techniques. The essential oil showed a chemical composition similar to that distilled from the leaves, but with some qualitative and quantitative differences regarding several minor compounds. The main constituents (>4%) were: δ-3-carene (24.23%), eudesm-7(11)-en-4-ol (13.02%), thujopsan-2-α-ol (11.90%), β-pinene (7.96%), valerianol (5.19%), and co-eluting limonene and β-phellandrene (4.47%). The volatile fraction was also submitted to enantioselective analysis on a β-cyclodextrin column, obtaining the separation and identification of the enantiomers for α-thujene, β-pinene, sabinene, α-phellandrene, limonene and β-phellandrene. Furthermore, the anti-fungal activity of non-volatile secondary metabolites was tested in vitro, with carnosol resulting in being very active against the “blast disease” caused by the fungus Pyricularia oryzae
Aislamiento Y Caracterización De Metabolitos Secundarios Mayoritarios Del Fruto De Annona Montana
No full textIn the present investigation the isolation of six was realized metabolitos secondary, from the fruit Annona Montana. The isolated compounds presented Rf of: a) 0.1, b) 0.2, c) 0.4, d) 0.6, e) 0.7, f) 0.9; fusion point: a) 280-290 °C, b) 109-130 °C, c) 310-320 °C, d) 194-200 °C, e) 248-253 °C, f) 235-240 °C. Of three extracts obtained with the solvents: hexane, ethyl acetate and methanol, selected the most cytotoxic previous evaluation of his activity in lines of human cancer, information considered from the results obtained in the Center of Cellular and Molecular Biology in the area of toxicological Genetics of Universidad Técnica Particular de Loja. The isolation realized it from the methanolic extract of the fruit, in view of his polar characteristic and to his considerable cytotoxic percentage opposite to lines of human cancer, in contrast, the extract hexane and ethyl acetate, they presented minor level of citotóxicidad. The division effected by means of column chromatography (CC) with the help of chromatography in thin cap (CCF) for the check of praise compounds isolated for it ascending polaridad used organic solvents; and as developers Sulfate Cérico and ultraviolet light (lamp UV of 254 and 366 nm). The purification of the majority compounds was realized from 320 resultant fractions of the division in chromatography in column of which there were obtained six fractions joined according to related characteristics and Rf exposed in chromatography of thin cap (CCF). The obtained results allow to know and to characterize compounds that constitute the medicinal plants with important traditional application as source of natural raw material
Actividad antifúngica de metabolitos secundarios aislados a partir de la especie Piper asperiusculum, “matico-matapiojo”, al Sur del Ecuador.
No full textSe recolectó muestra vegetal en estado de floración de Piper asperiusculum. A partir de 500g de hojas y flores secas, se obtuvo tres extractos: en acetato de etilo (LCH1), metanol (LCH2) y metanol/agua 7:3 v/v (LCH3). Para observar la riqueza de los metabolitos secundarios se realizaron cromatografía de capa fina (TLC) en fase directa e inversa.
Mediante Cromatografía en Columna se fraccionó 1,2g de extracto en acetato de etilo desclorofilado (LCH1.1) y se recolectaron 367 fracciones. Las fracciones que cristalizaron y mostraron similitud entre ellas según el análisis TLC fueron agrupadas y posteriormente analizadas por CG-EM logrando identificar tres compuestos conocidos: β-sitosterol, Stigmasta-5,22-dien-3-ol y Campesterol en las fracciones 176-195 (LF7) y en las fracciones 206-226 (LF9) se encontró mayoritariamente el 6β-Hydroxyestr-4-eno-3,17-diona.
Finalmente se determinó la actividad antifúngica mediante el método de Microdilución en Caldo que mide la Concentración Mínima Inhibitoria contra las cepas de Trichophyton rubrum ATCC 28188 ® y Trichophyton mentagrophytes ATCC 28185 ® resultando moderada (250μg/mL) para LCH1.1 y nula para LCH1, el residuo con clorofilas del extracto en AcEOt (LCH1.2) y para las fracciones LF7 y LF9
