1,721,104 research outputs found

    Ancient varieties of legumes: studies on the pathogenesis related genes-nematode interactions

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    The legumes are important protein source for the human, animal and soil health. Unfortunately these crops (e.g., chickpea), present low productivity, because of several biotic and abiotic stresses typical of the semi-arid tropical regions where they are mainly cultivated. Among biotic stresses, plant-parasitic nematodes have been estimated to be responsible for a 14% loss in annual yield. Root-knot nematodes, included Meloidogyne spp., and cyst-forming nematode (Heterodera spp.) can attack the legumes plant roots, and their symptoms are a consistent limitation in crop production. New improved cultural management practices have been adopted, moreover studies on the systemic acquired resistance (SAR) in plants, have been routinely use for the defense status of plants. PR proteins are the downstream components of SAR, being induced in response to attack by pathogens. Host plants are able to coordinate the expression of specific PR genes in response to infection by nematode, at the molecular level. Interactions studies between legumes and phytoparasitic nematodes have been conducted in order to 1) find out marker genes to detect the response induced by the nematode stress, 2) identify and characterize the Pathogenesis Related genes in Apulian ancient varieties of legumes, 3)analyze the expression of PR genes by means of qRT-PCR, in response to nematode infections

    Use of pelleted Medicago sativa meal for the control of root-knot and cyst nematodes

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    The nematicidal potential of soil amendments with pelleted alfalfa meal was investigated on the root-knot nematode Meloidogyne incognita on tomato and on the cyst nematode Heterodera carotae on carrot in two field experiments in Southern Italy. Application of 20 and 40 t ha-1 alfalfa pellets was compared with 30 L ha-1 quillay formulation and 30 L ha-1 emulsifiable phenamiphos, whereas nontreated soil was used as a control. Alfalfa pellets strongly suppressed soil population density of M. incognita and H. carotae compared to nontreated soil, with no significant difference from phenamiphos. Nematode infestation was significantly lower at the highest pellet dosage in the trial M. incognita, whereas no difference between the suppressivity of the two amendment rates was found on H. carotae. Both dosages of alfalfa pellets remarkably increased tomato and carrot yield and improved carrot tap root quality compared to nontreated soil and quillay extract treatment, but significantly less than phenamiphos. Based on the results from these experiments, soil amendments with pelleted alfalfa meal seems to represent a further valuable tool for a sustainable control of phytonematodes, also due to technical feasibility and positive effects on soil fertility

    Nematicidal activity of essential oils from aromatic plants of Morocco

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    Abstract Essential oils (EOs) from Moroccan ecotypes of Artemisia herba-alba, Citrus sinensis, Rosmarinus officinalis and Thymus satureioides and their main components were comparatively evaluated for their in vitro activity against the phytonematode species Meloidogyne incognita, Pratylenchus vulnus and Xiphinema index. Suppressiveness of drench or fumigation soil treatment with the four EOs was also investigated against M. incognita on tomato in potting mixes. The in vitro nematicidal activity of the main constituents of the four EOs was also determined. More than 94 % mortality of M. incognita juveniles occurred after a 24-h exposure to a 15 lg ml-1 solution of A. herba-alba EO. A 100 % mortality of X. index females was observed at all exposure times and concentrations of all EOs except that from C. sinensis. Mortality of P. vulnus peaked at 75 %only after a 96-h permanence in the 15 lg ml-1 solution of R. officinalis EO. The in vitro activity of EOs’ main components was never as high as that of the whole EOs, suggesting a synergistic action of the EO multicomponent mixture. Soil treatments with the four EOs generally resulted in a significant reduction of root-knot nematode infestation on tomato,though in an aggregate comparison of data the A. herba-alba EO was more active than the other three EOs, and fumigation treatments were more effective than application in water solution. The high nematicidal activity of most of the tested EOs and their large availability make them a potential source of new nematicide formulations
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