275 research outputs found
Domoic Acid
No full texteditors, R.H. Waring, G.B. Steventon, S.C. Mitchell.; Includes bibliographical references and index.; Chapter 4. written by R. Andrew R. Tasker - Domoic acid - UPEI professor, Dept. of Biomedical Sciences.Source type: Print(0
Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels
No full textMitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion
Seizures in 204 Comatose Children: Incidence and Outcome
No full textPurpose: Seizures are common in comatose children, but may be clinically subtle or only manifest on continuous electroencephalographic monitoring (cEEG); any association with outcome remains uncertain. Methods: cEEG (one to three channels) was performed for a median 42 h (range 2–630 h) in 204 unventilated and ventilated children aged 15 years (18 neonates, 61 infants) in coma with different aetiologies. Outcome at 1 month was independently determined and dichotomized for survivors into favourable (normal or moderate neurological handicap) and unfavourable (severe handicap or vegetative state). Results: Of the 204 patients, 110 had clinical seizures (CS) before cEEG commenced. During cEEG, 74 patients (36 %, 95 % confidence interval, 95 % CI, 32–41 %) had electroencephalographic seizures (ES), the majority without clinical accompaniment (non-convulsive seizures, NCS). CS occurred before NCS in 69 of the 204 patients; 5 ventilated with NCS had no CS observed. Death (93/204; 46 %) was independently predicted by admission Paediatric Index of Mortality (PIM; adjusted odds ratio, aOR, 1.027, 95 % CI 1.012–1.042; p 3 % fast, aOR 5.43, 95 % CI 1.90–15.6; excess slow with <3 % fast, aOR 8.71, 95 % CI 2.58–29.4; low amplitude, 10th centile <9 V, aOR 3.78, 95 % CI 1.23–11.7; and burst suppression, aOR 10.68, 95 % CI 2.31–49.4) compared with normal cEEG, as well as absence of CS at any time (aOR 2.38, 95 % CI 1.18–4.81). Unfavourable outcome (29/111 survivors; 26 %) was independently predicted by the presence of ES (aOR 15.4, 95 % CI 4.7–49.7) and PIM (aOR 1.036, 95 % CI 1.013–1.059). Conclusion: Seizures are common in comatose children, and are associated with an unfavourable outcome in survivors. cEEG allows the detection of subtle CS and NCS and is a prognostic tool.Version of Recor
Cyclopentyl-adenosine decreases caspase-3 activity and LDH release following simulated ischemia in cerebellar granule neurons
No full textActivation of adenosine A1 receptors in vivo or in vitro, prior to simulated ischemic insults have been demonstrated to be neuroprotective. Preconditioning with A1 receptor agonists, both in vivo and in vitro, has been shown to induce neuroprotective effects against necrosis even when administered 24–72 hours before ischemic insult. This thesis examined the neuroprotective effects of the selective A1 receptor agonist, N6-cyclopentyl-adenosine (CPA), against both apoptotic and necrotic cell death following simulated ischemia in cultured rat cerebellar granule neurons. Using antibodies directed towards neuronal nuclear protein and glial fibrillary acid protein, the purity of cerebellar granule cell cultures was found to be 98.6 ± 0.4%. This indicates that the results from these experiments were likely mediated by effects of the drugs and simulated ischemia on neurons and not astrocytes. To determine if the neuroprotection induced by CPA treatment was mediated by activation of A1 receptors, a concentration response curve for DPCPX was generated in the presence and absence of 1μM CPA. (Abstract shortened by UMI.).Source: Masters Abstracts International, Volume: 42-02, page: 0538.Advisers: Marva I. Sweeney-Nixon; Andrew Tasker
A congratulatory ode to Admiral Keppell. [electronic resource] : By the author of the Ode to the warlike genius of Great Britain.
No full textThe author of the Ode to the warlike genius of Great Britain = William Tasker.Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
From bedside to bench: The needs of clinically-relevant animal models of disease development
No full textOde to the warlike genius of Great Britain. By the Rev. W. Tasker, A.B. Dedicated to the Right Honourable Lord Amherst [electronic resource].
No full textThe titlepage is a cancel.Price from imprint: price One Shilling.Electronic reproduction.English Short Title Catalog,Reproduction of original from Huntington Library
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