175 research outputs found
Contribución al estudio de la parametritis : Tesis inaugural para optar al grado de doctor en medicina
No full textFil: Sundblad, Manuel. Universidad de Buenos Aires. Facultad de Medicina. Buenos Aires, Argentina.A la cabeza de portada: Universidad Nacional de la Capital. Facultad de Ciencias Médicas. - Incluye nómina de Catedráticos y Asignaturas. Tesis con dedicatoria
Mineralogy and geochemistry of indium-bearing polymetallic veins in the Sarvlaxviken area, Lovisa, Finland
No full textAbstract not availableMira Valkama, Krister Sundblad, Rune Nygård, Nigel Coo
Indium mineralisation in A-type granites in southeastern Finland: Insights into mineralogy and partitioning between coexisting minerals
No full textSeveral promising exploration targets in the western parts of the Wiborg batholith, southeastern Finland, have been studied with respect to Zn, In, Ag, As, Sn and Cu. Ores occur both as massive In-bearing magnetite-sphalerite (Getmossmalmen), as greisen-style veins (Jungfrubergen) and as Cu-dominant polymetallic quartz veins (Korsvik-1 and -2, Sarvlaxviken area). The Cu-dominant (chalcopyrite-bornite), Zn-poor quartz veins are characterised by high In/Zn ratios (>3000); roquesite (CuInS2) being a major indium-carrier, alongside sphalerite, chalcopyrite and arsenopyrite. In contrast, sphalerite is the dominant In-carrier in the greisen veins and massive ores characterised by lower In/Zn ratios (2 join beyond a couple of wt.% In (and Cu) in sphalerite. The co-existing roquesite is close to an ideal composition and contains μm-scale exsolutions of sphalerite, indicating negligible solid solution at the CuInS2 end of the join. The new data are compared with the published data on the mineralogical distribution of the indium in sulphide ores. © 2011 Elsevier B.V.N.J. Cook, K. Sundblad, M. Valkama, R. Nygård, C.L. Ciobanu, and L. Danyushevskyhttp://www.elsevier.com/wps/find/journaldescription.cws_home/503324/description#descriptio
Supporting information data files and R-script
No full textThis data package contains data files and R-script for:
Temperature moderates eDNA-biomass relationships in northern pike
*Ogonowski1, M., Karlsson1, E., Vasemägi1,2, A., Sundin1, J., Bohman1, P. , Sundblad1, G.
1Department of Aquatic Resources, Institute of Freshwater Research, Swedish University of Agricultural Sciences, Stångholmsvägen 2, SE-17893, Drottningholm, Sweden
2Chair of Aquaculture, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 46, 51006, Tartu, Estonia
Corresponding author: [email protected] </p
Lead isotopic systematics of massive sulphide deposits in the Urals: applications for geodynamic setting and metal sources
No full textLead isotopic compositions of 61 samples (55 galena, one cerussite [PbCO3] and five whole ore samples) from 16 Volcanic Hosted Massive Sulphide (VHMS) deposits in the Urals Orogeny show an isotopic range between 17.437 and 18.111 for 206Pb/204Pb; 15.484 and 15.630 for 207Pb/204Pb and 37.201 and 38.027 for 208Pb/204Pb. Lead isotopic data from VHMS deposits display a systematic increase in ratios across the Urals paleo-island arc zone, with the fore-arc having the least radiogenic lead compositions and the back-arc having the most radiogenic lead. The back arc lead model ages according to Stacey-Kramers model are close to the biostratigraphic ages of the ore-hosting volcano-sedimentary rocks (ca. 400 Ma). In contrast, less radiogenic lead from the fore-arc gives Neoproterozoic (~ 700 Ma) to Cambrian (480 Ma) lead model ages with low two-stage model ? values of 8.8 (parameter ? = 238U/204Pb reflects the averaged U/Pb ratio in the lead source), progressively increasing stratigraphically upwards to 9.4 in the cross-section of the ore-hosting Baymak-Buribai Formation. The range of age-corrected uranogenic lead isotopic ratios of the volcanic and sedimentary host rocks is also quite large: 206Pb/204Pb = 17.25-17.96; 207Pb/204Pb = 15.48-15.56, and generally matches the ores, with the exception of felsic volcanics and plagiogranite from the Karamalytash Formation being less radiogenic compare to the basaltic part of the cross-section, which would potentially imply a different source for the generation of felsic volcanics. This may be represented by older Neoproterozoic oceanic crust, as indicated by multiple Neoproterozoic ages of mafic-ultramafic massifs across the Urals. The relics of these massifs have been attributed by some workers to belong to the earlier Neoproterozoic stage of pre-Uralian ocean development. Alternative sources of lead may be Archean continental crust fragments/sediments sourced from the adjacent East-European continent, or Proterozoic sediments accumulated near the adjacent continent and presently outcropping near the western edge of Urals (Bashkirian anticlinorium). The contribution of Archean rocks/sediments to the Urals volcanic rock formation is estimated to be less than 0.1% based on Pb-Nd mixing models.The most radiogenic lead found in VHMS deposits and volcanics in the Main Uralian Fault suture zone, rifted-arc and back-arc settings, show similar isotopic compositions to those of the local Ordovician MORBs, derived from highly depleted mantle metasomatized during dehydrational partial melting of subducted slab and oceanic sediments. The metasomatism is expressed as high ? 207Pb/204Pb values relative to the average for depleted mantle in the Northern hemisphere, and occurred during the subduction of oceanic crust and sediments under the depleted mantle wedge. A seemingly much younger episode of lead deposition with Permian lead model ages (ca. 260–280 Ma) was recorded in the hanging wall of two massive sulphide deposits
Inmunological and molecular aspects of premature ovarian failure
No full textLa falla ovárica prematura (FOP), caracterizada por amenorrea hipergonadotrófica antes de los 40 años, afecta al 1% de las mujeres en edad fértil. Por su parte, el síndrome de ovario resistente (SOR) es propuesto como una forma folicular de FOP, caracterizado por la presencia de numerosos folículos primordiales en los ovarios. La FOP puede ser de origen autoinmune, iatrogénico, infeccioso, genético/cromosómico o metabólico, entre otros. Cuando no es posible identificar una causa para su desarrollo, la FOP es clasificada como idiopática. Nuestro estudio consistió en abordar el análisis de posibles causas inmunológicas y moleculares de la FOP. En trabajos previos detectamos mediante Western-blot la presencia de anticuerpos dirigidos hacia un antígeno ovárico de ~50 kDa en el 19,1% de un total de 110 pacientes FOP. En esta parte del estudio, trabajamos en la purificación e identificación de esta proteína. Mediante espectrometría de masa identificamos a la enolasa-α como el antígeno específico hacia el cual estarían dirigidos estos anticuerpos anti-ovario en pacientes FOP. Estos resultados fueron confirmados mediante Western-blots en los que se utilizó a la proteína recombinante como antígeno. Concluímos que la determinación de anticuerpos anti-enolasa-α podría significar un aporte importante para el diagnóstico de FOP autoinmune, y su utilización como marcador de autoinmunidad ovárica permitiría brindar a las pacientes un tratamiento acorde a la naturaleza autoinmune del síndrome. Asimismo, realizamos un estudio retrospectivo sobre el análisis de 247 pacientes FOP que habían sido derivadas a nuestro laboratorio para la determinación de la presencia de inmunoglobulinas dirigidas hacia el R-FSH (Ig-R-FSH). Encontramos que sólo las 23 mujeres que habían sido previamente diagnosticadas como SOR presentaban estos anticuerpos. Además, confirmamos que la actividad inhibitoria de las Ig-R-FSH puede ser clasificada en dos grupos: uno con efecto inhibitorio “irreversible”, cuya Ki aparente es 2,94 x 1012 M-1, mil veces mayor que la constante de afinidad de la FSH por su receptor, y el otro con un mecanismo inhibitorio “reversible”, con una Ki aparente similar a la constante de afinidad de la FSH por su receptor. Concluímos que la determinación de estos anticuerpos sería un instrumento importante para el diagnóstico del SOR, permitiendo realizar el diagnóstico principalmente sobre la base de estudios serológicos. Con respecto a las posibles causas genéticas de la FOP, el gen del R-FSH ha sido uno de los genes candidatos más estudiados. Hasta el presente se han descripto 9 mutaciones inactivantes en este gen. En nuestro laboratorio investigamos la presencia de mutaciones en el gen del R-FSH en 20 pacientes FOP, 5 de las cuales habían sido diagnosticadas como SOR, y 44 mujeres controles. Estudiamos la secuencia codificante completa del gen del R-FSH mediante PCR-SSCP, digestión con enzimas de restricción y/o secuenciación directa. No detectamos mutaciones en los exones 1 al 10 en ninguno de los individuos analizados. Además, observamos que los polimorfismos A919G y A2039G del exón 10 se presentan en dos posibles combinaciones, avalando la existencia dos isoformas del R-FSH: Ala307-Ser680 y Thr307-Asn680. Nuestros resultados sugieren que la variante alélica 919G-2039G (OR G vs A= 1,20; IC 95%= 0.53-2.71) o el genotipo homocigota (OR GG vs AG = 1,00; IC 95%= 0.22-4.46; OR GG vs AA = 1,40; IC 95%= 0.25-7.87) no estarían asociados al riesgo de desarrollo de FOP. Además, en dos individuos controles detectamos la presencia de la sustitución C1022T, en heterocigosis. En conclusión, sugerimos que las mutaciones en el gen del R-FSH son poco frecuentes en las pacientes FOP de Argentina. La presencia de una isoforma particular del R-FSH no estaría asociada a la enfermedad. Por su parte, el gen de la inhibina α (INHα) ha sido propuesto como otro gen candidato para el desarrollo de FOP, debido al rol de las inhibinas en la regulación de la FSH. En dos trabajos previos se sugiere que el polimorfismo C129T y la sustitución G769A estarían involucrados en la etiología de la FOP. En este trabajo analizamos 52 pacientes FOP; 14 presentaban FOP asociada a enfermedad/es autoinmune/s (FOP-EA) y las 38 restantes fueron consideradas idiopáticas (FOP-I). Además, estudiamos 136 mujeres controles, separadas en dos grupos: a) menores de 40 años (C40). No encontramos diferencias significativas en el riesgo de desarrollo de FOP para el alelo T del polimorfismo C129T, cuando comparamos FOP-I con C40 (OR T vs C; I-POF vs C40 = 1.07; IC 95% = 0.53-2.16). Obtuvimos resultados similares al comparar los genotipos (OR CC vs (CT+TT) ; I-POF vs C40 = 1.05; IC 95% = 0.43-2.56). La posible implicancia del polimorfismo C129T en los niveles serológicos de inhibinas fue analizada en un grupo de 42 mujeres controles. No hallamos diferencias significativas (p>0,05) entre los grupos CC y CT+TT al comparar los valores de los péptidos de inhibina de la fase folicular media (Pro-αC e Inhibina B), o los valores de la fase lútea media (Pro-αC e Inhibina A). Con respecto a la mutación G769A, hallamos 8/135 controles y 1/52 pacientes FOP (una FOP-EA) heterocigotas para dicha sustitución. La presencia de la variante G769A en un número relevante de individuos controles se describe en este trabajo por primera vez. Nuestros resultados indicarían que las variantes C129T y G769A no estarían asociadas al desarrollo de FOP. En conclusión: i) la proteína enolasa-α sería el el antígeno específico de ~50 kDa hacia el cual estarían dirigidos los anticuerpos anti-ovario en pacientes FOP; ii) las Ig-R-FSH se encontrarían presentes exclusivamente en las pacientes con diagnóstico de SOR; iii) el gen del R-FSH y las variantes C129T y G769A del gen INHα no estarían involucrados en la etiopatología de la FOP en pacientes argentinas.Premature ovarian failure (POF) is characterized by amenorrhea before the age of 40. POF is usually classified as “follicular” or “afollicular” form. Resistant ovary syndrome (SOR) is proposed as a follicular form of POF, with ovaries in which numerous primordial follicles are present. POF involves almost 1% of the western female population. It is a very heterogeneous syndrome, with a multicausal pathogenesis, and any of the following: chromosomal, enzymatic, iatrogenic, autoimmune or infectious aberration, may be the cause of the disease. In our laboratory, we focused our POF research on some immunological and genetic etiological factors. In previous studies, by Western-blot using ovarian cytosolic fraction antigens, we found that 19.1% of 110 POF patients showed specific reactivity with a ~ 50 kDa antigen. In the present work, we focused on the purification and identification of this protein. NanoLC-ESI-MS/MS identificated α-enolase as the specific antigen to which anti-ovarian antibodies are directed in these POF patients. These results were confirmed by Western-blot using recombinant human α-enolase. In conclusion, detection of anti- α-enolase antibodies by Western-blot could be a suitable marker for diagnosis of autoimmune POF. Its implementation as marker of ovarian autoimmunity may allow treatment of patients according to the immunological nature of their pathology. We also evaluated the presence of circulating immunoglobulins that inhibit FSH binding to its receptor (Ig-FSHR) by FSH-binding inhibition assay, in 247 patients with POF and 60 normally menstruating women (controls). We found that only those 23 patients that had been previously diagnosed as ROS presented Ig-FSHR. The remaining 224 POF patients and 60 control subjects proved negative. In addittion, our results clearly demonstrated that immunoglobulin fractions can be classified into two groups: one, with an “irreversible” nature of inhibitory effect, which Ki was 2.94 x 1012 M-1, a thousand times higher than the affinity constant for FSH-receptor (FSHR) binding interaction, and the other group with a “reversible” mechanism of inhibition, with an apparent Ki similar to the affinity constant for FSHR binding interaction. We concluded that determination of Ig-FSHR could be instrumental in diagnosing ROS, mainly upon the basis of serological findings. Regarding genetic factors, the FSHR gene has long be considered a strong candidate gene for POF. Nine mutations have been described in this gene to date. We investigated the presence of mutations and/or polymorphisms in FSHR gene, in 20 patients with POF, 5 of which were diagnosed as ROS, and 44 controls. The complete coding sequence was analyzed by PCR followed by SSCP, direct sequencing or restriction enzyme analysis. No mutations in FSHR gene were identified in the subjects studied. Polymorphisms A919G and A2039G of exon 10, cosegregated in all the homozygous individuals, indicating that FSHR presents two isoforms: Ala307-Ser680 and Thr307-Asn680. OR results suggested that the 919G-2039G allelic variant (OR G vs A= 1,20; IC 95%= 0.53-2.71) or the homozygous genotype (OR GG vs AG = 1,00; IC 95%= 0.22- 4.46; OR GG vs AA = 1,40; IC 95%= 0.25-7.87) is not associated to disease risk. In addition, a heterozygous substitution C1022T (Val341Ala) was found in two control subjects. We suggest that mutations in FSHR gene are rare in women with POF in Argentine. Presence of a particular FSHR isoform does not appear to be associated with this disease. On the other hand, inhibin-α gene is also proposed as a candidate gene for POF, due to its role in negative feedback control of hypophyseal FSH. In two previous studies, polymorphism C129T and substitution G769A of this gene were suggested to be involved in the etiology of POF. We studied 52 POF patients; 14 presented POF in association with autoimmune/s disease/s (AAD-POF), and the remaining 38 wereconsidered idiopathic (I-POF). As controls, 136 normal women were also studied, divided into two groups: a) controls below the age of 40 years (C40). We found no significant differences in risk of POF development for the T allele of polymorphism C129T when we compared I-POF either with C40 (OR T vs C; I-POF vs C40 = 1.07; IC 95% = 0.53-2.16). Similar results were found when genotypes were compared (OR CC vs (CT+TT); I-POF vs C40 = 1.05; IC 95% = 0.43-2.56). The implication of this polymorphism in serum inhibin levels was analyzed in 42 control women, and found no significant differences (p>0.05) between CC and CT+TT groups either when we compared mid-follicular phase inhibin peptide values (Pro-αC and Inhibin B) or when we compared mid-luteal phase values (Pro-αC and Inhibin A). Heterozygocity for the substitution G769A was found in 8/135 controls and only in 1/52 POF women (an AAD-POF). Presence of this substitution in a relevant number of control subjects is herein described for the first time. Our results might indicate that C129T and G769A variants in INH-α gene may not be associated to POF disease. In conclusion: i) α-enolase would be the specific antigen of ~50 kDa to which anti-ovarian antibodies are directed in POF patients; ii) Ig-FSHR would be present only in those patients diagnosed as ROS; c) neither FSHR gene nor C129T and G769A variants in INHα gene would be involved in the etiology of POF.Fil: Sundblad, Victoria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Credibility, community college, and the closet: how students perceive a gay music instructor
No full textIt has been shown that credibility beliefs impact classroom relationships such that
students are more likely to rate traditionally marginalized faculty members as less credible than others and are less likely to interact with faculty they find less credible. The purpose of this mixed-methods study, undergirded by Critical Realism (CR), was to examine students’ credibility beliefs about and perceived learning from a male community college music instructor whose sexual orientation was expressed differently in two quasi-experimental conditions. It is an extension of the work of Russ, Simonds, and Hunt (2002) and others (Boren & McPherson, 2018; De Souza & Olson, 2018).
The participants for this study were students enrolled in eight class sections of Music Appreciation at a large Mid-Atlantic community college. The same male guest lecturer expressed either a homosexual or heterosexual identity by mentioning his husband or wife by name during each otherwise identical lecture. Participants were then asked to complete McCroskey and Teven’s (1999) Source Credibility Measure to evaluate the lecturer on the credibility domains of competence, character, and caring.
Data showed that participants as a collective did not provide significantly different ratings on any dimension of credibility nor for perceptions of learning for the gay or straight instructor; however, additional analysis revealed deeper complexity with regard to participant beliefs. Specifically, younger participants provided higher ratings in the straight instructor condition and African American participants provided lower ratings in the gay instructor condition.
Open-ended prompts and interview data largely supported the statistical findings; however, they also revealed the presence of some discomfort with gay instructors and an eagerness to support a marginalized instructor. Quantitized open-ended and interview response data also showed that participants in the straight lecturer condition may have attended more to competence while participants in the gay lecturer condition may have attended more to positive character and caring traits. Implications of these results are discussed as they pertain to student course evaluations and teaching demonstrations for gay instructors
Idun (Årg. 7, N:r 18)
No full textChristina Larsson. Johannes Sundblad 137
Till min hustru. R. 139
Den moderna kvinnodräkten. Ett hinder för självständighet. (forts. och slut) Maria Bolin 139
Ett ord i vårstöksdagarna. Skånska i Småland 140
Flyktighet. Inga Schenfelt 140
För patience-amatörer. Tante Mathilda 141
Till den svenska näktergalens minne. Olivia Frigelius 141
Ur notisboken. 142
Teater och musik. 142
Kärleksbacillen. (forts.) Jonas Dugge 143
För de fattiga små barnen. Red. af Idun 144
Tidsfördrif. 14
Slowing of carotid-cardiac baroreflex with standing and with isometric and dynamic muscle activity
No full text We hypothesized that the carotid-cardiac baroreflex becomes slowed in conditions with increased sympathetic activity. Changes in heart rate (HR) and blood pressure in response to 10-s trains of 50-mmHg pulses of neck suction (NS) were studied in six male subjects during supine rest, upright rest, isometric arm exercise at 30% of maximum voluntary contraction, and dynamic leg exercise at 100 W in the sitting position. Estimated mean carotid distending pressure increased by approximately 20 mmHg with 50-mmHg, QRS-triggered, pulsatile NS. Repeated NS sequences were performed in each condition. The amplitude of the bradycardic response was highly variable among the subjects and did not differ significantly between conditions, mean values ranging from 0.3 to 0.6 beats.min-1.mmHg-1. In supine rest, the full bradycardic response appeared within < 1 s, i.e., during or immediately after the R-R interval of the first NS pulse. In the other conditions it took significantly longer, 2-3 s or three to seven R-R intervals, for the full HR responses to develop. Our results support the notion that the carotid-cardiac baroreflex in humans becomes slowed under conditions of concurrent sympathetic stimulation. </jats:p
Galectin-1: A Jack-of-all-trades in the resolution of acute and chronic inflammation
No full textRegulatory signals provide negative input to immunologicalnetworks promoting resolution of acute andchronic inflammation. Galectin-1 (Gal-1), a member of afamily of evolutionarily conserved glycan-binding proteins,displays broad anti-inflammatory and proresolvingactivities by targeting multiple immune cell types. Withinthe innate immune compartment, Gal-1 acts as a resolutionassociatedmolecular pattern by counteracting the synthesisof proinflammatory cytokines, inhibiting neutrophiltrafficking, targeting eosinophil migration and survival,and suppressing mast cell degranulation. Likewise, thislectin controls T cell and B cell compartments by modulatingreceptor clustering and signaling, thus serving asa negative-regulatory checkpoint that reprograms cellularactivation, differentiation, and survival. In this review,we discuss the central role of Gal-1 in regulatoryprograms operating during acute inflammation, autoimmunediseases, allergic inflammation, pregnancy, cancer,and infection. Therapeutic strategies aimed at targetingGal-1?glycan interactions will contribute to overcomecancer immunosuppression and reinforce antimicrobialimmunity, whereas stimulation of Gal-1?driven immunoregulatorycircuits will help to mitigate exuberant inflammation.Fil: Sundblad, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Morosi, Luciano Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentin
- …
