2,102 research outputs found
Stanescu v. State of Connecticut, et.al.
No full text"June 24, 1996."; Discusses Stanescu v. State of Connecticut, et.al (U.S.D.C., April 17, 1996, Civil No. 3:94cv01781).; Harvested from the web on 2/28/1
Antibody elution method for multiple immunohistochemistry on primary antibodies raised in the same species and of the same subtype
No full textAbstract: Search: >> Advanced Search Institution: Universiteit Antwerpen Bibliotheek | Sign In via User Name/Password Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact Originally published as JHC exPRESS on February 16, 2009. doi:10.1369/jhc.2009.953240 This Article Full Text Full Text (PDF) Supplemental Data for this article All Versions of this Article: jhc.2009.953240v1 57/6/567 most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Pirici, D. Articles by Stanescu, R. PubMed PubMed Citation Articles by Pirici, D. Articles by Stanescu, R. Social Bookmarking What's this? Journal of Histochemistry and Cytochemistry Volume 57 (6): 567-575, 2009 Copyright \ua9The Histochemical Society, Inc. Antibody Elution Method for Multiple Immunohistochemistry on Primary Antibodies Raised in the Same Species and of the Same Subtype Daniel Pirici, Laurentiu Mogoanta, Samir Kumar-Singh, Ionica Pirici, Claudiu Margaritescu, Cristina Simionescu and Radu Stanescu Department of Histology (DP,LM) and Department of Pathology (CM,CS), University of Medicine and Pharmacy Craiova, Craiova, Romania; Neurodegenerative Brain Diseases Group, Vlaams Instituut voor Biotechnologie, Department of Molecular Genetics, Laboratory of Neurogenetics, Institute Born Bunge, University of Antwerp, Antwerp, Belgium (DP,SK-S); and Emergency County Hospital 1, Craiova, Romania (IP,RS) Correspondence to: Daniel Pirici, MD, Department of Histology, University of Medicine and Pharmacy Craiova, Petru Rares Street 2, 200349 CraiovaDolj, Romania. E-mail: [email protected] Double or multiple antigen labeling in IHC classically relies on the existence of primary antibodies raised in different species or of different IgG isotypes to ensure the specific labeling with the secondary detection systems. However, suitable pairs of primary antibodies are not always available or the best choice (e.g., as diagnostic tools). During the last few years, several methods have been proposed to overcome this, but none of them offers the flexibility needed for reliable double or multiple enzymatic or fluorescent IHC. We present here a procedure that elutes the antibodies after a first round of immunolabeling, which, in combination with precipitation-based detection systems, allows multiple IHC rounds even for primary antibodies raised in the same species and IgG isotype. Compared with other proposed methods, this procedure ensures a reliable enzymatic or fluorescent staining without cross-reactivity and without loss of tissue antigenicity, thus offering a flexible tool for colocalization studies and pathological diagnosis. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials
Structural and electronic properties of thin Ni layers on Cu(1 1 1) as investigated by ARPES, STM and GIXD
No full textThe growth and the crystalline and electronic structure of Ni deposited on single crystalline Cu(1 1 1) were studied by scanning tunnelling microscopy (STM), grazing incidence X-ray diffraction (GIXD) and angle-resolved photoemission spectroscopy (ARPES). In the early stages of growth monoatomic-high flat Ni islands, partially covered by Cu migrating from the surface, are observed. Starting from a pseudomorphic epitaxial relationship the in-plane lattice parameter progressively relaxes with increasing coverage. For a 20 monolayer (ML) thick deposit the in-plane lattice parameter is still found halfway between the bulk Ni and Cu lattice parameters. ARPES data also rule out the layer-by-layer growth and provide the values of the Ni exchange splitting
Paragus romanicus Stanescu 1992
No full text<i>Paragus romanicus</i> Stănescu, 1992 <p> <i>P. romanicus</i>, dont les larves sont aphidiphages, est une espèce associée aux milieux ouverts (prairies non améliorées) xériques. On la retrouve également dans des clairières et lisières de formations boisées telles que les forêts thermophiles de chênes et de hêtres (Speight et al. 2015). L’habitat des adultes et des larves est encore mal connu. L’aire de distribution de cette espèces est très large puisqu’on la retrouve du centre de la France aux Alpes et vers le sud jusqu’en Roumanie, en Turquie et jusqu’en Méditerranée. Sa répartition suisse connue s’étend du plateau aux zones non alpines ainsi qu’au jura (Speight et al. 2015). Ce taxon est considéré par Speight et al. (2015) comme menacé et en déclin au niveau helvétique.</p> <p> Le fait que plusieurs individus de <i>P. romanicus</i> provenaient de pièges à émergence atteste que ce taxon peut effectuer son développement larvaire dans les SPB de type prairies extensives. Cette espèce, dont les larves sont aphidiphage (Speight et al. 2015), peut donc très probablement jouer un rôle fonctionnel pour les cultures adjacentes aux SPB. Elle s’ajoute à d’autres espèces du même type répertoriées dans le canton [<i>Episyrphus balteatus</i> (De Geer, 1776), <i>Paragus haemorrhous</i> Meigen, 1822, <i>Sphaerophoria scripta</i> (L., 1758)]. Ceci confirme que les habitats situés en périphérie des cultures (vignobles et grandes cultures) sont susceptibles d’augmenter la colonisation des milieux cultivés par des auxiliaires utiles à la lutte de certains ravageurs comme le soulignent Nicholls et al. (2001). L’importance fonctionnelle de cette espèce dépend en grande partie de la gestion des SPB (principalement de la fauche), étant donné qu’elle effectue une grande partie de son développement dans les SPB et que son stade larvaire est lié aux plantes herbacées présentes sur ces prairies.</p> <p> <i>P. romanicus</i> peut facilement être confondu avec <i>P. bicolor</i> (Fabricius, 1794). Les femelles de ces deux espèces sont pratiquement indiscernables. En revanche, les mâles se différencient sans ambiguïté à partir des critères présents sur les genitalia (Fig. 1). Chez <i>P. romanicus</i>, un processus de l’aedeage en forme de pointe est visible sur l’hypopygium observé en vue latérale. Cette pointe est totalement absente chez <i>P. bicolor</i>.</p>Published as part of <i>Bessat, Marie, Speight, Martin C. D. & Castella, Emmanuel, 2018, Addition de trois nouvelles espèces à la liste des Syrphidae (Diptera) du canton de Genève, pp. 73-78 in Entomo Helvetica 11</i> on page 76, DOI: 10.5169/seals-986025, <a href="http://zenodo.org/record/8034460">http://zenodo.org/record/8034460</a>
Análisis del indice de competitividad de la industria T&T de Costa Rica, Puerto Rico y Singapur para el período 2007-2019
No full textActualmente, el sector turístico se ha convertido en el centro de la
actividad económica de países y regiones, en el marco de la globalización. Este
se encuentra en permanente transformación, en función de las expectativas
cambiantes de los viajeros y turistas. En este sentido, se realiza el análisis el índice
de competitividad turística de los países Costa Rica, Singapur y Puerto Rico. Las
economías de estos países se distinguen por ser exportadores y por tener una
actividad turística significativa. Para el análisis se utilizan los resultados de los
WTTR del 2007 al 2019, aunque la metodología de cálculo ha cambiado en el
tiempo, Singapur se ha distinguido por estar en los más altos posicionamiento a
nivel global. Por otro lado, Costra Rica se distingue por encontrarse en los primeros
puestos de la región americana. Como resultado se despliegan los factores que han
favorecido la competitividad de estas naciones.Vásquez Stanescu, Carmen LuisaBolaño Truyol, Jorge Daniel-will be generated-orcid-0000-0002-2934-2843-60
Laboratory-scale photomineralisation of n-alkanes in aqueous solution, by photocatalytic membranes immobilising titanium dioxide
No full textKinetics of photocatalytic oxidation of methane, ethane, n-heptane, n-decane, and n-dodecane, to yield intermediates, and photomineralisation of intermediates, to yield carbon dioxide and water, was
studied in aqueous solution, by a laboratory-scale photoreactor and photocatalytic membranes immobilizing 30±3 wt.% of TiO2, in the presence of stoichiometric hydrogen peroxide as oxygen donor. The whole volume
of irradiated solution was 4.000±0.005 L, the ratio between this volume and the geometrical apparent
surface of the irradiated side of the photocatalytic membrane was 3.8±0.1 cm, and the absorbed power
0.30W/cm (cylindrical geometry). A kinetic model was used, by which mineralisation of substrate to CO2 was supposed to occur, by kinetic constants k1, through one single intermediate, mediating the behaviour
of all the numerous real intermediates formed in the path from the substrate to CO2 (kinetic constants of
formation of the latter being k2). A competitive Langmuirian adsorption of both substrate and “intermediate”
was also supposed to be operative, as expressed by apparent adsorption constants k1 and k2, possessing a, partly at least, kinetic significance. By Langmuir-Hinshelwood treatment of initial rate data, starting values
of the k and K couples were obtained, from which, by a set of differential equations, the final optimised
parameters, k1 and k1, k2 and K2, were calculated, able fit the whole photomineralisation curve, and not only
its initial segment, as the Langmuirian parameters do. The parameters of present work are critically compared
with those obtained in two preceding set of studies relative to n-alkanoic acids and to n-alkanols. They are
interpreted on the basis of a closer behaviour of hydrocarbons to alkanols, from the photocatalytic point
of view, than to carboxylic acids are. Discussion of limiting effective quantum yields, and their comparison
with maximum, theoretical values, are also carried out
Laboratory-scale photomineralization of n-alkanes in gaseous phase by photocatalytic membranes immobilizing titanium dioxide
No full textKinetics of photocatalytic oxidation of methane, ethane, and n-heptane, to yield intermediates, and photomineralization of intermediates, to yield carbon dioxide and water, was studied in the gaseous phase, at 308 +/- 2 K, by a laboratory-scale photoreactor and photocatalytic membranes immobilizing 30 +/- 3wt.% of TiO2, in the presence of aerosolized stoichiometric hydrogen peroxide as oxygen donor, and at a relative humidity close to 100%. The whole volume of irradiated solution was 4.000 +/- 0.005 L, the ratio between this volume and the geometrical apparent surface of the irradiated side of the photocatalytic membrane was 3.8 +/- 0.1 cm, and the absorbed power was 0.30 W/cm ( cylindrical geometry). The pinetic parameters of the present work substantially coincide with those of the same molecules previously studied in aqueous solution, within the limits of experimental uncertainty. Photocatalytic processes thus appear to be controlled by interface phenomena, which are ruled kinetically, and apparently also thermodynamically, by concentration gradients, independently on diffusion and other processes in the aqueous or gaseous bulk, if turbulence in these phases is adequately assured
Solar energy driven photocatalytic membrane modules for water reuse in agricultural and food industries. Pre-industrial experience using s-triazines as model molecules
No full textA membrane module, utilizing photocatalytic membranes, has been employed in a pilot plant,
in conditions of solar irradiation, to investigate photomineralisation of atrazine, propazine, terbutylazine,
symazine, prometryn, and ametryn, as model molecules of s-triazine herbicides, at a standard concentration
(1.0 ppm) simulating those of contaminated aquifers, by using ozone as oxygen supplier. Photocatalytic
composite membranes immobilised 30±3 wt.% of TiO2 and 6 wt.% of a synergic mixture of tri-(t-butyl)- and tri-(i-propyl)vanadate(V). Photomineralisation was followed by analysis of substrate disappearance, as such, and by total organic carbon (TOC) analysis. A four parameters kinetic model was employed, as set up in previous studies of this series, to interpret the whole photomineralisation curve. Quantum yields,
as indicative of catalytic and photocatalytic mechanisms, were evaluated satisfactorily: they are discussed,
and compared with those of previous studies on the same substrates, carried out in the same module, but
in conditions of practically monochromatic irradiation (254 nm) within the range of optical absorption of
semiconductor. Finally, in order to compare effectiveness of composite photocatalytic membranes, described
above, prepared by photografting, either in the presence or in the absence of an added photopromoter, as
well as that of metallic membranes, onto which the semiconductor without any photopromoter was present
as a 3–4 μm thick surface layer, directly produced on the nanotechnologically treated surface, with those of
other commercial materials, parallel experiments were carried out, by using commercial sheets in which the
semiconductor was immobilised, by a method based substantially on glueing by colloidal silica. All of these
comparison experiments were carried out at a laboratory scale, by using, in these experiments, dioxygen of
air, or ozone as oxygen donors
Older males whistle better: age and body size are encoded in the mating calls of a nest-building amphibian (Anura: Leptodactylidae)
No full textCourtship acoustic displays in anuran amphibians are energetically costly and risky, but have a major role in mating success since they encode relevant information regarding the caller’s identity and status. Age and size are essential traits shaping fitness, reproductive success and life-history strategies, and thus are expected to also have a role in courtship displays. We tested this assumption in a species of nest-building frogs, Leptodactylus bufonius, in northern Argentina. We conducted the first detailed quantitative description of the males’ mating calls and assessed the effects of biological traits (i.e., body size parameters and individual age) and local climate (i.e., air temperature and humidity) on the main acoustic features of these calls (i.e., call duration, inter-call duration, dominant frequency, and dominant frequency modulation). The calls were short (mean ± SE, 0.163 ± 0.004 s), whistle-like, single notes with harmonic structure. The dominant frequency (1381.7 ± 16.2 Hz) decreased with arm length (χ2 = 5.244, df = 1, p = 0.022) and had an upward modulation (456.4 ± 11.0 Hz) which increased with age (χ2 = 4.7012, df = 1, p = 0.030). Call duration and dominant frequency were the most static parameters at intra-individual level, indicating their role in individual recognition. Temperature and humidity shaped the temporal acoustic parameters, and the dominant frequency. Our findings suggest that the acoustic features of the mating calls in amphibians could promote female mate choice in relation to both size and age and open up new questions for future research: are females more attracted to older males, and what are the specific costs and benefits? We suggest that mating calls may direct female preferences toward males of certain size and age classes, ultimately shaping the life-history strategies in a given population. Finally, we found discrepancies in the mating calls of L. bufonius recorded from Corrientes and those previously described from other populations, which suggests that multiple species may have been recorded under the same name.Fil: Stanescu, F.. Ovidius University; RumaniaFil: Marquez, R.. Consejo Superior de Investigaciones Científicas. Museo Nacional de Ciencias Naturales; EspañaFil: Cogalniceanu, D.. Ovidius University; RumaniaFil: Marangoni, Federico. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin
Improving machine dynamics via geometry optimization
No full textThe central thesis of this paper is that the dynamic performance of machinery can be improved dramatically in certain cases through a systematic and meticulous evolutionary algorithm search through the space of all structural geometries permitted by manufacturing, cost and functional constraints. This is a cheap and elegant approach in scenarios where employing active control elements is impractical for reasons of cost and complexity. From an optimization perspective the challenge lies in the efficient, yet thorough global exploration of the multi-dimensional and multi-modal design spaces often yielded by such problems. Morevoer, the designs are often defined by a mixture of continuous and discrete variables - a task that evolutionary algorithms appear to be ideally suited for. In this article we discuss the specific case of the optimization of crop spraying machinery for improved uniformity of spray deposition, subject to structural weight and manufacturing constraints. Using a mixed variable evolutionary algorithm allowed us to optimize both shape and topology. Through this process we have managed to reduce the maximum roll angle of the sprayer by an order of magnitude , whilst allowing only relatively inexpensive changes to the baseline design. Further (though less dramatic) improvements were shown to be possible when we relaxed the cost constraint. We applied the same approach to the inverse problem of reducing the mass while maintaining an acceptable roll angle - a 2% improvement proved possible in this cas
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