1,099 research outputs found
slim shady is a novel allele of PHYTOCHROME B present in the T-DNA line SALK_015201
No full textAuxin is a hormone that is required for hypocotyl elongation during seedling development. In response to auxin, rapid changes in transcript and protein abundance occur in hypocotyls, and some auxin responsive gene expression is linked to hypocotyl growth. To functionally validate proteomic studies, a reverse genetics screen was performed on mutants in auxin-regulated proteins to identify novel regulators of plant growth. This uncovered a long hypocotyl mutant, which we called slim shady, in an annotated insertion line in IMMUNOREGULATORY RNA-BINDING PROTEIN (IRR). Overexpression of the IRR gene failed to rescue the slim shady phenotype and characterization of a second T-DNA allele of IRR found that it had a wild-type (WT) hypocotyl length. The slim shady mutant has an elevated expression of numerous genes associated with the brassinosteroid-auxin-phytochrome (BAP) regulatory module compared to WT, including transcription factors that regulate brassinosteroid, auxin, and phytochrome pathways. Additionally, slim shady seedlings fail to exhibit a strong transcriptional response to auxin. Using whole genome sequence data and genetic complementation analysis with SALK_015201C, we determined that a novel single nucleotide polymorphism in PHYTOCHROME B was responsible for the slim shady phenotype. This is predicted to induce a frameshift and premature stop codon at leucine 1125, within the histidine kinase-related domain of the carboxy terminus of PHYB, which is required for phytochrome signaling and function. Genetic complementation analyses with phyb-9 confirmed that slim shady is a mutant allele of PHYB. This study advances our understanding of the molecular mechanisms in seedling development, by furthering our understanding of how light signaling is linked to auxin-dependent cell elongation. Furthermore, this study highlights the importance of confirming the genetic identity of research material before attributing phenotypes to known mutations sourced from T-DNA stocks.This article is published as Dash, Linkan, Robert E. McEwan, Christian Montes, Ludvin Mejia, Justin W. Walley, Brian P. Dilkes, and Dior R. Kelley. "slim shady is a novel allele of PHYTOCHROME B present in the T‐DNA line SALK_015201." Plant Direct 5, no. 6 (2021): e00326. doi:10.1002/pld3.326.Iowa State University (ISU); National Science Foundation (NSF), Grant/Award Number: 1444503
Shady Oak Farm party attendees
No full textNote from James R. Record to Katrine Deakins with corrections to her previous list of Shady Oak Farm party Attendees.Mrs. Deakings: To correct your list
Letter re: Shady Oak Farm party
No full textLetter from Louis R. Sarazan, Controller at Schermerhorn Company, thanking Amon Carter for his visit to Shady Oak
Letter re: Shady Oak Farm party
No full textLetter from John R. Halsell Jr. of the Hartford Accident and Indemnity Company, thanking Amon Carter for the Shady Oak Party honoring American Airlines Officers and Directors
Mrs. Vance Abeldt, with R. A. Shady Lane
No full textMrs. Vance Abeldt, left, poses with a gun pointed at her boss, R. A. Shady Lane, a school tax assessor and collector, on Haskell\u27s Frontier Day, pretending to rob him. Fort Worth Star-Telegram.https://mavmatrix.uta.edu/specialcollections_startelegram1960s/5339/thumbnail.jp
Shady Oak Farm party seating instructions
No full textLetter from James R. Record to Katrine Deakins, secretary to Amon Carter, with seating instructions for a party at Shady Oak Farm honoring the Aviation Writers Association visit to the Consolidated aircraft plant
slim shady is a novel allele of PHYTOCHROME B present in the T‐DNA line SALK_015201
No full textAbstract Auxin is a hormone that is required for hypocotyl elongation during seedling development. In response to auxin, rapid changes in transcript and protein abundance occur in hypocotyls, and some auxin responsive gene expression is linked to hypocotyl growth. To functionally validate proteomic studies, a reverse genetics screen was performed on mutants in auxin‐regulated proteins to identify novel regulators of plant growth. This uncovered a long hypocotyl mutant, which we called slim shady, in an annotated insertion line in IMMUNOREGULATORY RNA‐BINDING PROTEIN (IRR). Overexpression of the IRR gene failed to rescue the slim shady phenotype and characterization of a second T‐DNA allele of IRR found that it had a wild‐type (WT) hypocotyl length. The slim shady mutant has an elevated expression of numerous genes associated with the brassinosteroid‐auxin‐phytochrome (BAP) regulatory module compared to WT, including transcription factors that regulate brassinosteroid, auxin, and phytochrome pathways. Additionally, slim shady seedlings fail to exhibit a strong transcriptional response to auxin. Using whole genome sequence data and genetic complementation analysis with SALK_015201C, we determined that a novel single nucleotide polymorphism in PHYTOCHROME B was responsible for the slim shady phenotype. This is predicted to induce a frameshift and premature stop codon at leucine 1125, within the histidine kinase‐related domain of the carboxy terminus of PHYB, which is required for phytochrome signaling and function. Genetic complementation analyses with phyb‐9 confirmed that slim shady is a mutant allele of PHYB. This study advances our understanding of the molecular mechanisms in seedling development, by furthering our understanding of how light signaling is linked to auxin‐dependent cell elongation. Furthermore, this study highlights the importance of confirming the genetic identity of research material before attributing phenotypes to known mutations sourced from T‐DNA stocks
Aviation writers at Shady Oak Farm
No full textPhoto of men at a dinner for visiting aviation writers at Shady Oak Farm. On reverse: "Cmdr. R. G. Mayer, standing [Harry] Woodhead, Amon Carter, [Bryant] Boatner, [I. M.] Laddon, [Melvin J.] Miller, seated Shady Oak Farm, June 7, 1947.
Aviation writers at Shady Oak Farm
No full textPhoto of Amon Carter with aviation writers at a dinner at Shady Oak Farm. On reverse: "On plane - Amon Carter and others in Aviation 20 years or longer Standing, l. to R. - [Russ] Brinkley, unidentified, unidentified, Davidoff (sic) [Alexis Dawydoff], [C. B.] Allen, unidentified, [Gerard B.]Dobben, unidentified, [Paul H.] Wilkinson, [Dick] Kirschbaum, unidentified, unidentified. Kneeling, l. to R. - [C. B.] Colby, [William S.] Friedman, [Ken] Fletcher, [Henry] Ward. Shady Oak Farm. June 7, 1947.
Shady Beach playground at Juanita, 1933
No full textWebster & Stevens acquired negatives of photographs taken by Charles R. Laidlaw, another of Seattle's commercial photographers. In this photo, taken by Laidlaw in 1933, children play on the swings, slide down the slide, and ride the rope merry-go-round at Juanita's Shady Beach.1 nitrate negative: b&w; 5 x 7 in
- …
