47 research outputs found
Severe liver disease in early childhood due to fibrinogen storage diseases and de novo gamma375ARG-->TRP gene mutation
No full textWe report hypofibrinogenemia and massive hepatic storage of fibrinogen in a child with cryptogenic chronic liver disease. Fibrinogen gene analysis revealed a de novo Aguadilla (c.1201C>T; p.Arg375Trp) mutation. This mutation should be considered in childhood hypofibrinogenemia associated with chronic liver disease
The human olfactory system: stimulation and statistical data applying functional magnetic resonance imaging(fMRI) in healthy and congenitally anosmic subjects
No full textSurgical and Percutaneous Aortic Valve Replacement Have Different Impact on Diastolic Phase in Elderly Patients With Aortic Valve Stenosis
No full textNew basic insights on the potential of a chitosan-based medical device for improving functional recovery after radical prostatectomy
No full textFunctional Magnetic Resonance Imaging (fMRI) study and evaluation of effective connectivity model of the human olfactory system
No full textNew basic insights on the potential of a chitosan‐based medical device for improving functional recovery after radical prostatectomy
No full textObjectives: To evaluate: (i) the neuro-regenerative potential of chitosan membrane (CS-Me) on acutely axotomised autonomic neurones in vitro; (ii) to exclude the possibility that a pro-regenerative biomaterial could interfere with the proliferation activity of prostate cancer cell lines; (iii) to provide an in vivo proof of the biocompatibility and regeneration promoting effect of CS-Me in a standardised rat model of peripheral nerve injury and repair; (iv) finally, to evaluate the tissue reaction induced by the degrading material; as previous studies have shown promising effects of CS-Me for protection of the neurovascular bundles for potency recovery in patients that undergo nerve-sparing radical prostatectomy (RP). Materials and methods: Addressing aim (i), the neuro-regenerative potential, organotypic cultures derived from primary sympathetic ganglia were cultured on CS-Me over 3 days and neurite extension and axonal sprouting were evaluated. Addressing aim (ii), effects of CS on cancer cells, different human prostate cancer cell lines (PC3, DU-145, LN-Cap) were seeded on CS-coated plates or cultured in the presence of CS-Me dissolution products. Addressing aims (iii) and (iv), functional recovery of peripheral nerve fibres and tissue reaction with the biomaterial, CS-Me and CS nerve guides were used to repair a median nerve injury in the rat. Functional recovery was evaluated during the post-recovery time by the behavioural grasping test. Results: CS-Me significantly stimulated axon elongation from autonomic ganglia in comparison to control conditions in organotypic three-dimensional cultures. CS coating, as well as the dissolution products of CS-Me, led to a significantly lower proliferation rate of prostate cancer cell lines in vitro. Tissue reaction towards CS-Me and standard CS nerve guides was similar in the rat median nerve model, as was the outcome of nerve fibre regeneration and functional recovery. Conclusion: The results of this study provide the first experimental evidence in support of the clinical safety of CS-Me and of their postulated effectiveness for improving functional recovery after RP. The presented results are coherent in demonstrating that acutely axotomised autonomic neurones show increased neurite outgrowth on CS-Me substrate, whilst the same substrate reduces prostate cancer cell line proliferation in vitro. Furthermore, CS-Me do not demonstrate any disadvantage for peripheral nerve repair in a standard animal model
Functional Magnetic Resonance Imaging (fMRI) of the Human Olfactory System: Stimulation and Statistical Data Analysis in Normosmic and Congenital Anosmic Subjects
No full textCharacterization of cells from invaded lymph nodes in patients with solid tumors. Lymphokine requirement for tumor-specific lymphoproliferative response
No full textExtensive molecular, phenotypic and geographic characterization of natural populations of the wild relative Triticum urartu occurring throughout the Fertile Crescent
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